LOCUS       sequence-384377-        6019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  image COR1C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Striepen JF, Voeltz GK
  TITLE     Coronin 1C restricts endosomal branched actin to organize ER contact
            and endosome fission.
  JOURNAL   J Cell Biol. 2022 Aug 1;221(8). pii: 213342. doi: 
            10.1083/jcb.202110089. Epub 2022 Jul 8.
  PUBMED    35802042
REFERENCE   2  (bases 1 to 6019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2022-08-1"; volume: "221(8). pii: 213342. doi"; 
            pages: " 10.1083/jcb.202110089. Epub 2022 Jul 8"
COMMENT     Sequence Label: SiRES COR1C ACT-DCC-GFP
FEATURES             Location/Qualifiers
     source          1..6019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1966..2685
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     polyA_signal    2807..2928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2844..2863)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2898..2917
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2935..3390)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3072..3093)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3284..3303
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3417..3521
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3523..3880
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3523..3714
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3546..3566)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3731..3866
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3915..4709
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3915..4709
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3969..3988)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4579..4598
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4897..4916)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4941..4988
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5317..5905
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5806..5825
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagat gaggcgagtg gtacgacaga gtaaattccg ccacgtgttt
      661 gggcaagcgg tgaagaatga ccagtgctat gatgacatcg acgtttctcg tgtgacctgg
      721 gatagttcct tttgtgctgt caatcccaga tttgttgcca taatcataga ggcaagtggg
      781 ggaggagcgt tccttgtcct ccctctgcat aaaaccgggc gcatcgacaa atcttaccct
      841 acagtatgtg gccacacagg accagtgctg gacatagact ggtgcccaca taacgatcag
      901 gtcattgcca gcggttcaga ggactgcacg gtcatggtat ggcagatccc agaaaatgga
      961 ctcacccttt ccctgactga acctgtggtg attttggaag gccactcaaa gagagtcggc
     1021 atcgtggctt ggcatccaac ggcccgcaat gtgcttctta gtgcaggctg tgataatgcc
     1081 attatcatct ggaatgtggg aacaggggaa gcccttataa acttggacga tatgcattca
     1141 gacatgattt acaatgtgag ctggaaccgg aatggcagtc tgatctgcac agcttccaaa
     1201 gacaagaaag tgagagtcat tgatcccagg aaacaagaga ttgttgctga gaaggagaaa
     1261 gcacatgaag gagcaagacc catgagagcc atcttcctgg ccgatggcaa tgtcttcacc
     1321 actgggttca gccgcatgag cgagcggcag ctggctctct ggaatccgaa aaatatgcag
     1381 gaaccaattg ctcttcatga gatggacact agcaatgggg tgttgctgcc tttctatgac
     1441 cctgacacca gcatcattta cttatgtgga aagggtgaca gcagtattcg ctattttgag
     1501 atcacggatg aatccccgta cgtccactac ctcaacacat tcagcagcaa ggagcctcag
     1561 agagggatgg gttacatgcc caagagggga cttgatgtta acaaatgtga gattgccaga
     1621 ttcttcaaac ttcatgagag aaagtgtgaa cctattatta tgactgttcc caggaagtct
     1681 gaccttttcc aagatgacct gtatcctgac acagcggggc cagaggccgc gctggaggca
     1741 gaagagtggt tcgaaggcaa gaatgcagac ccaatcctca tctccttgaa gcacgggtac
     1801 attccaggca aaaatagaga cctgaaagtc gtcaagaaga acattctgga tagcaagccc
     1861 actgcaaacg aggagtgcga cctgatcagc atccccgagg aaaccacaga cacggccagt
     1921 gtgcaaaatg aagccaagtt ggatgagatt cgggatccac cggtcatggt gagcaagggc
     1981 gccgagctgt tcaccggcat cgtgcccatc ctgatcgagc tgaatggcga tgtgaatggc
     2041 cacaagttca gcgtgagcgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     2101 aagttcatct gcaccaccgg caagctgcct gtgccctggc ccaccctggt gaccaccctg
     2161 agctacggcg tgcagtgctt ctcacgctac cccgatcaca tgaagcagca cgacttcttc
     2221 aagagcgcca tgcctgaggg ctacatccag gagcgcacca tcttcttcga ggatgacggc
     2281 aactacaagt cgcgcgccga ggtgaagttc gagggcgata ccctggtgaa tcgcatcgag
     2341 ctgaccggca ccgatttcaa ggaggatggc aacatcctgg gcaataagat ggagtacaac
     2401 tacaacgccc acaatgtgta catcatgacc gacaaggcca agaatggcat caaggtgaac
     2461 ttcaagatcc gccacaacat cgaggatggc agcgtgcagc tggccgacca ctaccagcag
     2521 aataccccca tcggcgatgg ccctgtgctg ctgcccgata accactacct gtccacccag
     2581 agcgccctgt ccaaggaccc caacgagaag cgcgatcaca tgatctactt cggcttcgtg
     2641 accgccgccg ccatcaccca cggcatggat gagctgtaca agtgagcggc cgcgactcta
     2701 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     2761 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     2821 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     2881 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg
     2941 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     3001 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     3061 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     3121 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     3181 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     3241 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     3301 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     3361 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     3421 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3481 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca
     3541 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     3601 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     3661 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     3721 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     3781 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     3841 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
     3901 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     3961 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4021 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4081 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4141 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4201 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4261 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4321 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4381 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     4441 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     4501 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     4561 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     4621 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     4681 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     4741 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     4801 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     4861 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
     4921 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
     4981 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     5041 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     5101 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
     5161 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5221 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5281 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5341 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5401 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5461 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5521 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5581 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5641 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5701 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5761 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5821 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5881 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5941 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6001 ccgtattacc gccatgcat
//
