LOCUS       sequence-38438-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Guizetti J, Schermelleh L, Mantler J, Maar S, Poser I, Leonhardt H, 
            Muller-Reichert T, Gerlich DW
  TITLE     Cortical constriction during abscission involves helices of 
            ESCRT-III-dependent filaments.
  JOURNAL   Science. 2011 Mar 25;331(6024):1616-20. Epub 2011 Feb 10.
  PUBMED    21310966
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-03-25"; volume: "331(6024)"; pages: "1616-20. Epub 2011 
            Feb 10"
COMMENT     Sequence Label: CHMP6_GFP
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    30..54
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(658..682)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(742..793)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(771..791)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tctcgccttg ctacatggtg gcgaccggta ccactttgta caagaaagtt gggtacgaag
       61 ctgccaccag ctccgcctgc ctgggcctgg ccttgacagg gacgttttct gggatcttct
      121 caggaagggg ctcggaggga acctctggca gctctatttg ttcctgagtg attgcgctca
      181 gctcctccag gatggcgtct tcatcctcct gagtgaagct tcctgccagg agctcgtcta
      241 tttgccgctg gtactccacg gcctcctgcg tctcgtccag gatcctctcc acctcttcaa
      301 tggacatcac ctggtgcatc ttgttcagac actcatttcc aaactgcagc ccctccatca
      361 ctttcatttc gatctgggtg aactcaatac tctgaaccat ggcctccagg ctgctgatct
      421 ggttctccgt cctgtccagg agctgctcct ggtatcgctt cttcttgagc agcagcttgg
      481 cccgttcctt cctgccgtcc cgcagcagct gccgggccag ggcgcgctcg cgctccagct
      541 gctgggcgat cctcttctgg tactgcctca gcttgtcccg ctgctgcttc agttgcagga
      601 tggccttgtc ctgctccgtg acgcggctct gcttcttgcg gccgaacagg ttacccatgc
      661 caactttttt gtacaaactt gtaagcttga gctcgagatc tgagtccggt agcgctagcg
      721 gatctgacgg ttcactaaac cagctctgct tatatagacc tcccaccgta cacgcctacc
      781 gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgantttg
      841 gtgccaaaac aaactcccat tgacgtcaat ggggtggaga ctnggaaatc cccgtgagtc
      901 aancngctat ccacgcccat
//
