LOCUS       sequence-38439-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Guizetti J, Schermelleh L, Mantler J, Maar S, Poser I, Leonhardt H, 
            Muller-Reichert T, Gerlich DW
  TITLE     Cortical constriction during abscission involves helices of 
            ESCRT-III-dependent filaments.
  JOURNAL   Science. 2011 Mar 25;331(6024):1616-20. Epub 2011 Feb 10.
  PUBMED    21310966
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-03-25"; volume: "331(6024)"; pages: "1616-20. Epub 2011 
            Feb 10"
COMMENT     Sequence Label: CHMP2A_GFP_N_term
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    32..56
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(723..747)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(807..858)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(836..856)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tctcgccctt gctaccatgg tggcgaccgg taccactttg tacaagaaag ttgggcagtc
       61 cctccgcagg ttcttaagcc gttcctccag gtctgcatca gcatcagcta gggctgaggc
      121 tgcggcctct gcttttttcc caccagcagc cacactaagc gagcccccag ttgaggggag
      181 gttcgacagc tcatctgtta ggctaagtcc cagctcatcc agaacctggg acaccacagc
      241 atcactctcc tcttcatctt cctcatcacc catggcatca tcaatggcat cattcatcat
      301 ctcctccttc atatccatga tctctgcctg ccgctcaaac tccatcatga tcttctggat
      361 ctggggcaac ttcagctgtc tgttcatggt gcccatggcc ttggtgacac ccttcatggc
      421 ttgtgccatc gagttgttgg acttgagtgt ctggatcttg agggacacag cctggatgtt
      481 ggcccgcatc aatacaaact tgcgcacata gcgccgggtg cgcaccaagt cttttgccat
      541 gatgcgaaca gcatccatct ggccttgctt ggccatcttc ttaatgtctg caatgatttt
      601 cttctcctgg gtctctagtt tctgtcgctc gcggtccagc tcccgcatgg cacggttcag
      661 ggccctctgg ttctgccgca gtagctcctc tggcgtcttc cggcgcccga acaataggtc
      721 catgccaact tttttgtaca aacttgtaag cttgagctcg agatctgagt ccggtagcgc
      781 tagcggatct gacggttcac taaaccagct ctgcttatat agacctccca ccgtacacgc
      841 ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
//
