LOCUS       sequence-384391-        9213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Kemmler CL, Moran HR, Murray BF, Scoresby A, Klem JR, Eckert RL, 
            Lepovsky E, Bertho S, Nieuwenhuize S, Burger S, D'Agati G, Betz C, 
            Puller AC, Felker A, Ditrychova K, Botschi S, Affolter M, Rohner N, 
            Lovely CB, Kwan KM, Burger A, Mosimann C
  TITLE     Next-generation plasmids for transgenesis in zebrafish and beyond.
  JOURNAL   Development. 2023 Mar 28:dev.201531. doi: 10.1242/dev.201531.
  PUBMED    36975217
REFERENCE   2  (bases 1 to 9213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2023 Mar 28:dev.201531. doi: 10.1242/dev.201531."
COMMENT     Sequence Label: pCK005 ubi:mCerulean_ubb-polyA
FEATURES             Location/Qualifiers
     source          1..9213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    37..57
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3592..3616
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3639..3648
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3645..3647,3648..3650,3651..4163)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(3690..3711)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3951..3970)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4298..4319
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4381..4405)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     4413..4429
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5016..5032)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5017..5033)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(5050..5070)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(5078..5094)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5110..5244
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5134..5153)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5188..5207
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(5683..5870,5871..5882)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5916..5932)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5969..5989)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5969..5987)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6008..6024)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6021..6043)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6032..6048
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6056..6062,6063..6080,6081..6086))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6101..6122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6239..6256)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6410..6998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6490..6509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7169..7960,7961..8029))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7792..7811
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8030..8134)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8297..8318)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(8667..8678,8679..8841)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttggct
       61 ccgaattcgc ccttaaaact cgagaccagc aaagttctag aatttgtcga aacatttatg
      121 ttatatattt cctgaaaaaa attctgagta agttcttaag tgttattgcc agcaacataa
      181 acaacagacg gcaaaatgaa taaatgataa caaagcagta ggcttaaata aacctaattt
      241 ttataggctg ttctctacaa ccctcaaaca gtgattagtt ttgtacttat aaacttgccc
      301 tttcattcat atttcaagaa aattggttca gaagatctgg atattctagc agttgttcaa
      361 gctcatggag ggatcagtga cctgattcca caatgactag gcctaatcca gaaattagat
      421 gactgtcaac ataaaaaggc acagcactca ctagctgccc tatatatttt attatatttt
      481 acatatatta ttttatttat ttagctctga gtgctgtact ttctggttaa agaaaactgc
      541 ttacaacagc taacctgtac tacctcaggc tcagggaatt tggaacaggt ttgtctggtt
      601 tgtttcttta accatgcatg cttgttttca actatggcaa cacagtcaca tgggacatta
      661 cagaaatgat ttgtcgatga catgcgactt ttctttaata aagcgcaaag atcccaaaaa
      721 gcaaactttt aacaaaaatc atataattat attttcaatc cagctttgta gcaactttgt
      781 gctgctgttc actcagcaac agatagtcag tataaggtca gtgtgtctca aagcagtgcc
      841 atctgtttca cacattgcgt tctatatata agtgtgctgg ttgacacgac actgtataag
      901 gcctaggcta aaacacaaac aatgtagaat gacactgtgt tttttttgta aacaaatgtt
      961 gtttttggtt aaacatcttt gtgaaaacat cctcctgtca tgtatttgct atattcaaat
     1021 gttaaacccg tgcagaatag aacatataca aaaaaaaaca acacaacaca tttttaaaca
     1081 ttattaaata tcaagtattg ctggcagttc tgtttctgtt ttacagtacc ctttgccaca
     1141 gttctccgct tttcctggtc cagattccac aagtctgatt caccaatagc aaagcgaata
     1201 aacaaccaaa gcagccaatc actgcttgta gactgtcctg cgagaccggc ccattccagc
     1261 acattctgga aacttccttt atatgataat tataaataca tttaaattat tgatacaaaa
     1321 catgtaattc ctagaacata accatagcaa tcattagttt tcagggtaat tatgtatttt
     1381 taggatttga ctgcggaaag atctggtcat gtgacgtctc atgaacgtca cggccctggg
     1441 tttctataaa tacagtagga ctctcgacca tcggcagatt tttcgaagaa gaagatcagt
     1501 ttcaggagcc gtactgttcc gtttcaacgc aaatattaac ggtaagagcg aatttcctag
     1561 tttgttttca tgccattctt taaaaccata gcgtattact ttaattatag taaactttcg
     1621 ctttctttat tacaagagac gttttgtgtt gattctccgc ggacattttc ggtcagacaa
     1681 tcagaaaatg accgcggagg accagtaact tgcattacac gtaagttaaa tcttcgtgta
     1741 ttaaaatggt taggttgtta acgtcaaata ggttaccgtg tttgcgtgtg atcaggttgg
     1801 ttttgttaga tttttgtcag tatttttaat ttatttgttt tagtttattt attttttttg
     1861 ctgaatcata gtttgtgaac aaagaacccg gatgttacat acagtacagc cgccatgtta
     1921 cagagagtta taacttaatc attttaaaaa taattttgcc ttacttttag tttgtcatgt
     1981 tgagaaatga ggaaatgtta aaatgaggaa atatccaatt aatttaatat atcaaaataa
     2041 tccatgatta caatgcactg aactggagaa aattaagatg ttttctagtg tcatgaaaca
     2101 aatgtaagag atgtacattg tagatgtttt atgtcaagaa ttggctagtt gatgcagcat
     2161 actggcgata ctcagttgta ataacagtaa cgttacatgt taatagacta ctgagtatgc
     2221 tgttctgtct atgtatgctc tgtaagctac gagaaggact tttttaaaca gtaaagggtg
     2281 caatattttt acaaattgaa ttaaataaag gctgtctatt aagtaatatg cttgatattt
     2341 ttcttacttg atcggaaata agaaaaaata taaacgttgt tgctctaaaa atcctagttc
     2401 agtttagcca accacaaata cctttttgtt cctccaacag tttttttttc ttctctataa
     2461 tatttggcag tctatagtac tccaaatgtt tccccacagt ctaactaatt ggtacagcca
     2521 aaatcatgac acttattgca ataataattt tggttcattg gcattgttga tagcctgtgc
     2581 cactaatatg gtcgattgat catgcttcag gaagaaaact atattgtttg atgtaagatt
     2641 attaaatctt cacctgcttc cattacaaac tattcccatc ttattgaatt ctggtatgtc
     2701 ttaaaggatt agttcacttc ccaaatcaaa atttacttag ccttttttca tccatgatcc
     2761 ctttttttca tcattaatga agaaattgtt tttgaaaaag tttcaagatt tttttctcta
     2821 tattgtggag cttgttagtt taaaattcca aaatgcaata tgtggcttca aatggttcta
     2881 aatgatccca gtcaaggaat aacagtctta tctaatgaaa ccattagacc tttaaaaata
     2941 aaaaaaataa aagtatttat ttttaaatga ctgagtgatt aagttgaatt tcagcgtttc
     3001 cttactgtgt agaagtcctt ccttactggc cccacccttt ggttctctgc caatctgcta
     3061 cctaatgtaa tgttgtggaa cattattatt ctttatttct taatttttta ttttttattt
     3121 taaaacaatg taaactgcac agatgtgcag tttgtttaaa atggccaatg ctttggaaat
     3181 gcatgacata attagatttc atgatgcaca aagccaaatc tcagagcttg tgcaaaatga
     3241 gctatcattt cactaggtaa gaccctaaat tttcatatag gatcatttgg acaattttgc
     3301 tgcaggtaaa atgcattcta tagtccactg tcagccattg ttttggatag tatttatttt
     3361 tctctacaag tatagtcaat agttttctat tattttaaag gtttgtaaca tttaagggtg
     3421 accaaatgca aagtaaaatt tcattttcgg gtgaactatc tcgtttaaca tgggagaagt
     3481 gcaaaacata cattattggc tagaacattg tagtattttt taaatggaaa tgtgtgattg
     3541 ctaatcttac tttgaatttg tttacaggga tcctttaagg gcgaattcga cccaagtttg
     3601 tacaaaaaag caggctccgc ggccgccccc ttagatccgc caccatggtg agcaagggcg
     3661 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     3721 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     3781 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     3841 cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
     3901 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     3961 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     4021 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacg
     4081 ccatcagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact
     4141 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     4201 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     4261 ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     4321 ccgccgccgg gatcactctc ggcatggacg agctggccct cgagaagggt gggcgcgccg
     4381 acccagcttt cttgtacaaa gtgggcggcc gctctagaac tagtggatct tctagaaaga
     4441 ttgagtaagg tagaaccgac agtcttaggg atggacatta aattctcagt atcccctgct
     4501 tatgcactca ccactttttc ccattatttt attcatattg tgttgctaaa tccaactaca
     4561 ttcgtattac catgttcttt tattagtttt tttttatctc agctgtcaaa ataaaagcat
     4621 tgaaatacaa cattgttcta tttttcattg tggttttgca tgcccggtaa ataaaaaaac
     4681 aaggtaatga aaataagatg cgaaatgaga agtatctcat tatgctgtaa gaagcataat
     4741 tagaatatgc aagtagcttt ccaaatttta ggtgtcttgt ctttctgggc ttttaaagtg
     4801 tttattcatg tatatttata gatgggaatt tttctgctta ctgctttaaa atgttatagc
     4861 agctgctgaa ctttggtggt tctctttatt gtgttgtatg aacacagaaa atggctaaaa
     4921 atcgctaaca cttgatggca atgaattcat cttgctgaaa aactcgagcc atccggaaga
     4981 tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct cgaggggggg
     5041 cccggtaccc aactttatta tacatagttg ataattcact ggccgtcgtt ttacggtacc
     5101 atcgatgatg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     5161 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     5221 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     5281 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     5341 gattatgatc ctctagatca gatctgcgaa gatacggcca cgggtgctct tgatcctgtg
     5401 gctgattttg gactgtgctg ctcgcagctg ctgatgaatc acatacttcc tccattttct
     5461 tccactgatt gactgttata atttccctaa tttccaggtc aaggtgctgt gcattgtggt
     5521 aatagatgtg acatgacgtc acttccaaag gaccaatgaa catgtctgac caatttcata
     5581 taatgtgaaa acgattttca taggcagaat aaataacatt taaattaaac tgggcatcag
     5641 cgcaattcaa ttggtttggt aatagcaagg gaaaatagaa tgaagtgatc tccaaaaaat
     5701 aagtactttt tgactgtaaa taaaattgta aggagtaaaa agtacttttt tttctaaaaa
     5761 aatgtaatta agtaaaagta aaagtattga tttttaattg tactcaagta aagtaaaaat
     5821 ccccaaaaat aatacttaag tacagtaatc aagtaaaatt actcaagtac tttacacctc
     5881 tggttcttga ccccctacct tcagcaagcc cagcagatcc actagttcta gagcggccgc
     5941 caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
     6001 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6061 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6121 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6181 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6241 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6301 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6361 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6421 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6481 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6541 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6601 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6661 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6721 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6781 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6841 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6901 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6961 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7021 cggggtctga cgctcagtgg aacgaaaact cacgttaagg cattttggtc atgagattat
     7081 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7141 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7201 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7261 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7321 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7381 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7441 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7501 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7561 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7621 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7681 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7741 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7801 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7861 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7921 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7981 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttctttt
     8041 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8101 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta
     8161 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     8221 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     8281 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     8341 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca cgctggctaa gaactcatca
     8401 gcctccccgg tccatctacc cacgtaccaa tgcaccaatt ggccacaatg acggctacta
     8461 catggtgcca ttccttcctc tttataggaa tggagactac ctcctgtcca acaatgctct
     8521 tggatacgag tacgcctacc tgttggaccc aggtcattgc acaacaccag aaatgccctc
     8581 tgatctgcaa aagacgtgaa tatctgttca gacacccata tccactctgt tccacacagg
     8641 tcagaggttt gtccaggagt tcttgacaga ggtgtaaaaa gtactcaaaa attttactca
     8701 agtgaaagta caagtactta gggaaaattt tactcaatta aaagtaaaag tatctggcta
     8761 gaatcttact tgagtaaaag taaaaaagta ctccattaaa attgtacttg agtattaagg
     8821 aagtaaaagt aaaagcaaga aagaaaacta gagattcttg tttaagcttt taatctcaaa
     8881 aaacattaaa tgaaatgcat acaaggtttt atcctgcttt agaactgttt gtatttaatt
     8941 atcaaactat aagacagaca atctaatgcc agtacacgct actcaaagtt gtaaaacctc
     9001 agatttaact tcagtagaag ctgattctca aaattgttag tgtcaagcct agctcttttg
     9061 gggctgaaaa gcaatcctgc agtgctgaaa agcctctcac aggcagccga tgcgggaaga
     9121 ggtgtattag tcttgataga gaggctgcaa atagcaggaa acgtgagcag agactccctg
     9181 gtgtctgaaa cacaggccag atgggccctc gag
//
