LOCUS       sequence-384401-       11634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus, expresses Cre recombinase, mouse PVR (CD155), and 
            mScarlet-SIINFEKL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11634)
  AUTHORS   Freed-Pastor WA, Lambert LJ, Ely ZA, Pattada NB, Bhutkar A, Eng G, 
            Mercer KL, Garcia AP, Lin L, Rideout WM 3rd, Hwang WL, Schenkel JM, 
            Jaeger AM, Bronson RT, Westcott PMK, Hether TD, Divakar P, Reeves 
            JW, Deshpande V, Delorey T, Phillips D, Yilmaz OH, Regev A, Jacks T
  TITLE     The CD155/TIGIT axis promotes and maintains immune evasion in 
            neoantigen-expressing pancreatic cancer.
  JOURNAL   Cancer Cell. 2021 Oct 11;39(10):1342-1360.e14. doi: 
            10.1016/j.ccell.2021.07.007. Epub 2021 Aug 5.
  PUBMED    34358448
REFERENCE   2  (bases 1 to 11634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2021.07.007"; journalName: "Cancer Cell"; date: 
            "2021-10-11- 11"; volume: "39"; issue: "10"; pages: "1342-1360.e14"
COMMENT     Sequence Label: LV-PGK-PVR-P2A-Cre-EFS-mScarletSIIN
FEATURES             Location/Qualifiers
     source          1..11634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2638..3137
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2673..2696)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3070..3089
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      3168..3177
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4407..4463
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4464..4484
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4485..5513
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     promoter        5569..5780
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      5825..5834
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5831..6526
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(5964..5982)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6232..6251
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6614..6637
                     /codon_start=1
                     /product="antigenic CD8+ T cell epitope from chicken 
                     ovalbumin"
                     /label=OVA peptide
                     /translation="SIINFEKL"
     misc_feature    6726..7314
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6779..6799)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7197..7208)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7386..7619
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7645..7662)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7651..7875
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7921..8349
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8008..8027)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8218..8239
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8358..8378)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8363..8692
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8543..8678
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8605..8624
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8740..8787
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8806..9180
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9310..9431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9347..9366)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9401..9420
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9480..9496)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9480..9496)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9493..9515)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9504..9520
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9528..9534,9535..9552,9553..9558))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9573..9594
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9711..9728)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9882..10470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9962..9981)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10641..11432,11433..11501))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11264..11283
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11502..11606)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgat cagtgtgagg gagtgtaaag ctggtttggg
     2641 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     2701 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     2761 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     2821 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtg
     2881 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     2941 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     3001 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     3061 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     3121 catctccggg cctttcgcta actcgaacgc tagctgtgcg atcgtttgcc accatggctc
     3181 aactcgcccg agccacccgc tccccgctgt catggctgct gctgctgttc tgctatgcac
     3241 tccggaaagc gggtggggat atacgtgtgc tggtgcccta caattcgaca ggcgtcttgg
     3301 gagggtcgac caccttgcac tgtagtctga cttctaatga gaatgtgact atcactcaaa
     3361 taacctggat gaagaaggat tcaggtggat cccacgctct tgtggctgtc ttccacccca
     3421 agaaggggcc caacatcaaa gagccagaga gggtgaaatt cttggctgcc caacaggatc
     3481 tgaggaacgc atctctggcc atctcgaact taagtgtaga agacgaaggc atctatgaat
     3541 gtcagattgc cacattcccc agaggcagta gaagcaccaa tgcctggctg aaggtgcaag
     3601 cccgacctaa gaacactgca gaggccctgg agccctctcc caccttgata ctgcaggatg
     3661 tggctaaatg catctctgcc aatggtcacc ctcctggacg aatctcttgg ccctcgaatg
     3721 tgaatggaag tcaccgtgaa atgaaggaac cagggtccca gccgggcacc accacagtta
     3781 ccagctacct ctccatggta ccttctcgcc aggcagacgg caagaacatc acctgcacgg
     3841 tggagcatga aagcttacag gagctggacc agctgctggt gaccctttcc caaccctatc
     3901 cacctgaaaa cgtgtccatc tctggctatg acggcaactg gtatgttggc ctcactaact
     3961 tgaccctgac ctgtgaagct cacagcaaac cagcgcctga catggctgga tataactgga
     4021 gcacgaacac gggtgacttt cccaactctg ttaagcgcca gggcaatatg cttctaatct
     4081 ccaccgtaga ggatggtctc aataacacgg tcattgtgtg cgaagtcacc aatgccctag
     4141 ggtctgggca gggccaagtg cacatcattg ttaaagagaa acctgagaat atgcagcaaa
     4201 atacaagatt acacctaggc tacatctttc ttatcgtctt tgtcctcgct gtagtcatca
     4261 tcatcgcagc actatacact atacgaagat gcaggcatgg tcgtgctctg cagtccaatc
     4321 cctcagagag ggagaacgtc cagtattcat ctgtgaacgg cgactgtaga ctgaacatgg
     4381 agccaaacag cacaaggggc agcggcgcca ccaacttcag cctgctgaag caggccggcg
     4441 acgtggagga gaaccccggc ccccccaaga agaagaggaa ggtgtccaat ttactgaccg
     4501 tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc
     4561 tgatggacat gttcagggat cgccaggcgt tttctgagca tacctggaaa atgcttctgt
     4621 ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg tttcccgcag
     4681 aacctgaaga tgttcgcgat tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa
     4741 ctatccagca acatttgggc cagctaaaca tgcttcatcg tcggtccggg ctgccacgac
     4801 caagtgacag caatgctgtt tcactggtta tgcggcggat ccgaaaagaa aacgttgatg
     4861 ccggtgaacg tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac caggttcgtt
     4921 cactcatgga aaatagcgat cgctgccagg atatacgtaa tctggcattt ctggggattg
     4981 cttataacac cctgttacgt atagccgaaa ttgccaggat cagggttaaa gatatctcac
     5041 gtactgacgg tgggagaatg ttaatccata ttggcagaac gaaaacgctg gttagcaccg
     5101 caggtgtaga gaaggcactt agcctggggg taactaaact ggtcgagcga tggatttccg
     5161 tatctggtgt agctgatgat ccgaataact acctgttttg ccgggtcaga aaaaatggtg
     5221 ttgccgcgcc atctgccacc agccagctat caactcgcgc cctggaaggg atttttgaag
     5281 caactcatcg attgatttac ggcgctaagg atgactctgg tcagagatac ctggcctggt
     5341 ctggacacag tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga gtttcaatac
     5401 cggagatcat gcaagctggt ggctggacca atgtaaatat tgtcatgaac tatatccgta
     5461 acctggatag tgaaacaggg gcaatggtgc gcctgctgga agatggcgat tagaaaccgc
     5521 tgttcctagg aatcccgagg ccttcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     5581 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     5641 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     5701 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     5761 ggtttgccgc cagaacacag gtgtcgtgac gcgggacccg acattagcgc tacagcttaa
     5821 gcgggccacc atggtgagca agggcgaggc agtgatcaag gagttcatgc ggttcaaggt
     5881 gcacatggag ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg
     5941 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     6001 ctcctgggac atcctgtccc ctcagttcat gtacggctcc agggccttca ccaagcaccc
     6061 cgccgacatc cccgactact ataagcagtc cttccccgag ggcttcaagt gggagcgcgt
     6121 gatgaacttc gaggacggcg gcgccgtgac cgtgacccag gacacctccc tggaggacgg
     6181 caccctgatc tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat
     6241 gcagaagaag acaatgggct gggaagcgtc caccgagcgg ttgtaccccg aggacggcgt
     6301 gctgaagggc gacattaaga tggccctgcg cctgaaggac ggcggccgct acctggcgga
     6361 cttcaagacc acctacaagg ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga
     6421 ccgcaagttg gacatcacct cccacaacga ggactacacc gtggtggaac agtacgaacg
     6481 ctccgagggc cgccactcca ccggcggcat ggacgagctg tacaaggaga gcctgaagat
     6541 atctcaagct gtccatgcag cacatgcaga aatcaatgaa gcaggcagag aggtggtagg
     6601 ggagcagctc gagagtataa tcaactttga aaaactgtaa aaacgttgtt gtttggggtt
     6661 gaattactct acatgtttaa gggttccggt tccactaggt acaattcgat atcaagctta
     6721 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6781 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6841 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6901 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6961 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     7021 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7081 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     7141 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7201 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7261 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac
     7321 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     7381 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     7441 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7501 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7561 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7621 ggcccggcga aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     7681 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     7741 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     7801 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     7861 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     7921 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7981 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     8041 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     8101 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     8161 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     8221 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     8281 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     8341 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     8401 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     8461 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     8521 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8581 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     8641 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     8701 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     8761 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     8821 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     8881 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     8941 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     9001 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     9061 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     9121 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     9181 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     9241 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     9301 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9361 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9421 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     9481 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9541 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9601 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9661 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9721 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9781 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9841 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     9901 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9961 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    10021 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    10081 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    10141 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    10201 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    10261 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    10321 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10381 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    10441 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10501 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10561 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10621 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10681 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10741 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    10801 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    10861 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    10921 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    10981 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    11041 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    11101 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    11161 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    11221 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    11281 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    11341 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    11401 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    11461 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    11521 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    11581 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
