LOCUS       sequence-384404-        7765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Dilworth FJ, Seaver KJ, Fishburn AL, Htet SL, Tapscott SJ
  TITLE     In vitro transcription system delineates the distinct roles of the 
            coactivators pCAF and p300 during MyoD/E47-dependent 
            transactivation.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Aug 10. 101(32):11593-8.
  PUBMED    15289617
REFERENCE   2  (bases 1 to 7765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Aug 10. 101(32):11593-8."
COMMENT     Sequence Label: pFB-Flag-MyoD-E12
FEATURES             Location/Qualifiers
     source          1..7765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     regulatory      141..150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3371..3505
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3408..3427)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3462..3481
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3534..3699
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3883..4337
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3970..3989)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4179..4200
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4363..4467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4468..4536,4537..5328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4686..4705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5499..6087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5988..6007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6241..6258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  6392..6616
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(6683..7216)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7386..7391,7392..7408,7409..7414))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctgggccc gccaccatgg actacaaaga cgatgacgat ggatccatgg
      181 agcttctatc gccgccactc cgggacatag acttgacagg ccccgacggc tctctctgct
      241 cctttgagac agcagacgac ttctatgatg atccgtgttt cgactcacca gacctgcgct
      301 tttttgagga cctggacccg cgcctggtgc acgtgggagc cctcctgaaa ccggaggagc
      361 acgcacactt ctctactgcg gtgcacccag gcccaggcgc tcgtgaggat gagcatgtgc
      421 gcgcgcccag cgggcaccac caggcgggtc gctgcttgct gtgggcctgc aaggcgtgca
      481 agcgcaagac caccaacgct gatcgccgca aggccgccac catgcgcgag cgccgccgcc
      541 tgagcaaagt gaatgaggcc ttcgagacgc tcaagcgctg cacgtccagc aacccgaacc
      601 agcggctacc caaggtggag atcctgcgca acgccatccg ctacatcgaa ggtctgcagg
      661 ctctgctgcg cgaccaggac gccgcgcccc ctggcgccgc tgccttctac gcacctggac
      721 cgctgccccc aggccgtggc agcgagcact acagtggcga ctcagacgcg tccagcccgc
      781 gctccaactg ctctgatggc atgatggatt acagcggccc cccaagcggc ccccggcggc
      841 agaatggcta cgacaccgcc tactacagtg aggcggtgcg cgagtccagg ccagggaaga
      901 gtgcggctgt gtcgagcctc gactgcctgt ccagcatagt ggagcgcatc tccacagaca
      961 gccccgctgc gcctgcgctg cttttggcag atgcaccacc agagtcgcct ccgggtccgc
     1021 cagagggggc atccctaagc gacacagaac agggaaccca gaccccgtct cccgacgccg
     1081 cccctcagtg tcctgcaggc tcaaacccca atgcgattta tcaggtgctt ggtacggggg
     1141 gtggatccgg cgggggttct ggaggtggga gcggcggagg gtccggcgga ggttacccaa
     1201 tgatgaacca gtctcagaga atggcacccg tgggctctga caaggaactg agtgacctac
     1261 tggacttcag catgatgttc ccgctacctg tggccaatgg gaagagccgg cccgcctccc
     1321 tcgggggaac ccagtttgca ggctcaggac tggaggaccg acccagctca ggctcctggg
     1381 gcagcagtga ccagaacagt tcttcctttg accctagccg gacatacagc gaaggtgccc
     1441 acttcagtga ctcccacagc agcctgccgc cttccacgtt cctaggagct gggcttggag
     1501 gcaagggcag tgagcggaat gcctatgcca cctttgggag agacaccagt gttggcacct
     1561 tgagtcaggc tggcttcctg ccaggtgagc tgagcctcag cagtcccggg ccactgtccc
     1621 catcgggcat caagagcagc tcccagtatt acccctcatt ccccagcaac cctcgtcgga
     1681 gagctgcaga tggtggcctg gatactcagc cgaagaaggt ccggaaggtt ccgcctggtc
     1741 tcccttcctc ggtgtatccg cccagctcag gtgacagcta cagcagggat gctgcagcct
     1801 acccctccgc caagaccccc agcagcgctt acccctcccc cttctacgtg gcagatggca
     1861 gcctgcaccc atcagctgag ctctggagta cgcctagcca ggtgggcttt gggcccatgc
     1921 taggtgacgg ctcttcccct ctgccccttg caccgggcag cagctccgtg ggcagtggta
     1981 cctttggggg cctccagcag caggatcgca tgggctacca gctgcatgga tctgaggtta
     2041 atggctcgct cccagctgta tccagctttt cggctgcccc tggcacttac agtgggactt
     2101 ccggccacac gccccctgtg agtggggccg cagctgaaag cctcctaggc acccgaggga
     2161 ctacagccag cagctcaggg gatgcccttg ggaaggcact ggcctcgatc tactccccgg
     2221 atcactccag caataatttc tcacctagcc cctcaacgcc tgtgggttca ccccagggcc
     2281 tgccagggac atcacagtgg ccccgggcag gagcgcccag tgccttatcc cccaactacg
     2341 atgcaggtct ccatggcctg cagagcaaga tggaggaccg cttggacgag gccatccatg
     2401 tcctgcgaag ccacgctgtt ggcaccgcta gcgatctcca tgggcttttg cctggccatg
     2461 gcgcactgac cacgagcttc accggcccca tgtcactggg cgggcggcat gccggcctgg
     2521 tcgggggaag ccatcctgag gagggcctca caagtggggc cagtcttttg cataaccatg
     2581 ccagcctccc cagccagccc agttccctcc ctgacctctc acagagacct cccgactcct
     2641 atagtggact cgggagggca ggcacaacag cgggtgccag cgagatcaag cgggaggaga
     2701 aagaggatga ggaaatcgca tcagtagccg acgccgaaga ggacaagaag gacctgaagg
     2761 tcccacgcac gcgcaccagc ccagacgagg acgaggacga ccttctcccc ccagagcaga
     2821 aggcggagcg ggagaaggag cgccgggtgg ccaataatgc ccgagagcgc ctgcgggtcc
     2881 gcgacatcaa tgaggccttt aaggagctcg gccgcatgtg ccagctgcac ctcagcagcg
     2941 agaagccgca gaccaaactg ctcatcctgc accaggccgt ggccgtcatc ctcagcctgg
     3001 agcagcaggt gcgagaacgc aacctgaacc ccaaagcagc ctgcttgaag cggagggagg
     3061 aggagaaggt gtctggcgtg gtcggggacc cacagctggc cctgtcagcc gcccacccgg
     3121 gcctgggtga ggcccacaac ccagccgggc acctgtgaga attcaaaggc ctacgtcgac
     3181 gagctcacta gtcgcggccg ctttcgaatc tagagcctgc agtctcgagg catgcggtac
     3241 caagcttgtc gagaagtact agaggatcat aatcagccat accacatttg tagaggtttt
     3301 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     3361 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     3421 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     3481 caatgtatct tatcatgtct ggatctgatc actgcttgag cctaggagat ccgaaccaga
     3541 taagtgaaat ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg
     3601 ctacacccag ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt
     3661 tccacccctc ccagttccca actattttgt ccgcccacag cggggcattt ttcttcctgt
     3721 tatgttttta atcaaacatc ctgccaactc catgtgacaa accgtcatct tcggctactt
     3781 tttctctgtc acagaatgaa aatttttctg tcatctcttc gttattaatg tttgtaattg
     3841 actgaatatc aacgcttatt tgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     3901 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3961 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4021 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4081 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4141 tttttcgccc tttgacgttg gagtccacgt tcttaatagt ggactcttgt tccaaactgg
     4201 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4261 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     4321 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4381 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4441 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4501 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4561 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4621 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4681 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     4741 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4801 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     4861 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4921 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4981 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5041 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5101 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5161 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5221 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5281 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5341 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5401 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5461 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5521 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5581 caagagctac caactctttt tccgaaggta actggctgca gcagagcgca gataccaaat
     5641 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5701 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5761 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5821 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5881 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5941 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6001 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6061 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6121 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6181 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     6241 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     6301 ctgtgcggta tttcacaccg catagaccag ccgcgtaacc tggcaaaatc ggttacggtt
     6361 gagtaataaa tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga
     6421 acaaaataga tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg
     6481 aaatcagtcc agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac
     6541 tcttcatttt ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ccaagggcat
     6601 ggtaaagact atattcgcgg cgttgtgaca atttaccgaa caactccgcg gccgggaagc
     6661 cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca aagtgcatca
     6721 cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca ccgtaatctg
     6781 cttgcacgta gatcacataa gcaccaagcg cgttggcctc atgcttgagg agattgatga
     6841 gcgcggtggc aatgccctgc ctccggtgct cgccggagac tgcgagatca tagatataga
     6901 tctcactacg cggctgctca aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg
     6961 cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag ttcccgaggt
     7021 aatcggagtc cggctgatgt tgggagtagg tggctacgtc tccgaactca cgaccgaaaa
     7081 gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat gtgcgaatga
     7141 tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc gtaacatcgt
     7201 tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc
     7261 gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt
     7321 taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc
     7381 attacagttt acgaaccgaa caggcttatg tcaactgggt tcgtgccttc atccgtttcc
     7441 acggtgtgcg tcacccggca accttgggca gcagcgaagt cgaggcattt ctgtcctggc
     7501 tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca ggcattggcg gccttgctgt
     7561 tcttctacgg caaggtgctg tgcacggatc tgccctggct tcaggagatc ggaagacctc
     7621 ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga agtggttcgc atcctcggtt
     7681 ttctggaagg cgagcatcgt ttgttcgccc aggactctag ctatagttct agtggttggc
     7741 tacgtatact ccggaatatt aatag
//
