LOCUS       sequence-38443-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Weinberg DE, Nakanishi K, Patel DJ, Bartel DP
  TITLE     The inside-out mechanism of dicers from budding yeasts.
  JOURNAL   Cell. 2011 Jul 22;146(2):262-76.
  PUBMED    21784247
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-07-22"; volume: "146(2)"; pages: "262-76"
COMMENT     Sequence Label: pRSF-KpDcr1delta2d(E224Q)
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             105..398
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             517..528
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 aataattttg tttaacttta ataaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccacatggc aagcatgtcg gactcagaag
      121 tcaatcaaga agctaagcca gaggtcaagc cagaagtcaa gcctgagact cacatcaatt
      181 taaaggtgtc cgatggatct tcagagatct tcttcaagat caaaaagacc actcctttaa
      241 gaaggctgat ggaagcgttc gctaaaagac agggtaagga aatggactcc ttaagattct
      301 tgtacgacgg tattagaatc caagctgatc agacccctga agatttggac atggaggata
      361 acgatattat tgaggctcac agagaacaga ttggtggatc caacgagtta aaagttagag
      421 aattttatag actccacaat gcttgtgtga aattgaaaga gtctattaaa ttgatttatg
      481 aaaacccatt ggtaactgac caaaatgttt tgaatttggg aactgcagaa aatactattg
      541 attatacaat tttaaacaca cctacactaa atgttgctaa aactttactg ggcaatagat
      601 attcattaga tctaatagat ctttttcaat ctcatgattt caaagattca aacactgatg
      661 tggatatgtt tattaaatat cctgtggttt atgatgaaaa tttggaaaat ttagcattta
      721 tgcataaaag ttttccaaat atgaatgcac atctaaatga tgctcaaaag actcaattat
      781 ctaatgaaag gttagaattt t
//
