LOCUS       sequence-38445-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Weinberg DE, Nakanishi K, Patel DJ, Bartel DP
  TITLE     The inside-out mechanism of dicers from budding yeasts.
  JOURNAL   Cell. 2011 Jul 22;146(2):262-76.
  PUBMED    21784247
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-07-22"; volume: "146(2)"; pages: "262-76"
COMMENT     Sequence Label: pRSF-KpDcr1deltaNdeltaC
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             105..398
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 aataattttg tttaacttta ataaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccacatggc aagcatgtcg gactcagaag
      121 tcaatcaaga agctaagcca gaggtcaagc cagaagtcaa gcctgagact cacatcaatt
      181 taaaggtgtc cgatggatct tcagagatct tcttcaagat caaaaagacc actcctttaa
      241 gaaggctgat ggaagcgttc gctaaaagac agggtaagga aatggactcc ttaagattct
      301 tgtacgacgg tattagaatc caagctgatc agacccctga agatttggac atggaggata
      361 acgatattat tgaggctcac agagaacaga ttggtggatc caaatatcct gtggtttatg
      421 atgaaaattt ggaaaattta gcatttatgc ataaaagttt tccaaatatg aatgcacatc
      481 taaatgatgc tcaaaagact caattatcta atgaaaggtt agaatttttg ggtgatagtt
      541 ggctaggtgc tttagtttca tatattgtat acaccagatt tccttctgca aatgaaggta
      601 tgctttctca aatgaaagaa tcaatagtaa ataataataa tctatttgat tggagtacaa
      661 aattaaactt caccaagaga ttacaaggta atatcgctac acctactaga gtggtcaaag
      721 ataaaatgtc taaacgttat gctgattgtg ttgaagccta tattggtgca ttggttattg
      781 atagatttgg cactgagtt
//
