LOCUS       sequence-384450-       18965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rosa26 targeting vector, expresses dCas9-VPR and mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18965)
  AUTHORS   Freed-Pastor WA, Lambert LJ, Ely ZA, Pattada NB, Bhutkar A, Eng G, 
            Mercer KL, Garcia AP, Lin L, Rideout WM 3rd, Hwang WL, Schenkel JM, 
            Jaeger AM, Bronson RT, Westcott PMK, Hether TD, Divakar P, Reeves 
            JW, Deshpande V, Delorey T, Phillips D, Yilmaz OH, Regev A, Jacks T
  TITLE     The CD155/TIGIT axis promotes and maintains immune evasion in 
            neoantigen-expressing pancreatic cancer.
  JOURNAL   Cancer Cell. 2021 Oct 11;39(10):1342-1360.e14. doi: 
            10.1016/j.ccell.2021.07.007. Epub 2021 Aug 5.
  PUBMED    34358448
REFERENCE   2  (bases 1 to 18965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2021.07.007"; journalName: "Cancer Cell"; date: 
            "2021-10-11- 11"; volume: "39"; issue: "10"; pages: "1342-1360.e14"
COMMENT     Sequence Label: R26-dCas9-VPR-mNG
FEATURES             Location/Qualifiers
     source          1..18965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..1092
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    join(1107..1172,1173..1210)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     enhancer        1224..1603
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1883..2899
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2823..2845
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2907..2926
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2954..2987
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3154..3288
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3408..3542
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3662..3796
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    3825..3858
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3944..3953
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3950..3976,3977..3979,3980..6463,6464..6469,6470..6535,
                     6536..6538,6539..8053)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8066..8086
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    8123..8147
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8177..8326
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             8351..8371
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8801..9157
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             9176..9745
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             9755..9811
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9812..10522
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      10067..10076
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    10553..11141
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10606..10626)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11024..11035)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11144..11160)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11162..11179)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11168..11375
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    11408..15748
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(15833..15856)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(16107..16127)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(16107..16125)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(16146..16162)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16146..16162)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(16159..16181)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    16170..16186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(16194..16200,16201..16218,16219..16224))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16239..16260
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(16377..16394)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(16548..17136)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16628..16647)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(17307..18098,18099..18167))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17930..17949
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(18168..18272)
                     /gene="bla"
                     /label=AmpR promoter
     gap             18298..18310
                     /estimated_length=13
     rep_origin      complement(18338..18766)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(18448..18469)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     18660..18679
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     18893..18915
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     18907..18924
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     18908..18924
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     18934..18953
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        18934..18952
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 caccgcggca ggccctccga gcgtggtgga gccgttctgt gagacagccg ggtacgagtc
       61 gtgacgctgg aaggggcaag cgggtggtgg gcaggaatgc ggtccgccct gcagcaaccg
      121 gagggggagg gagaagggag cggaaaagtc tccaccggac gcggccatgg ctcggggggg
      181 ggggggcagc ggaggagcgc ttccggccga cgtctcgtcg ctgattggct tcttttcctc
      241 ccgccgtgtg tgaaaacaca aatggcgtgt tttggttggc gtaaggcgcc tgtcagttaa
      301 cggcagccgg agtgcgcagc cgccggcagc ctcgctctgc ccactgggtg gggcgggagg
      361 taggtggggt gaggcgagct ggacgtgcgg gcgcggtcgg cctctggcgg ggcgggggrg
      421 gggrgggrgg gtcagcgaaa gtagctcgcg cgcgagcggc cgcccaccct ccccttcctc
      481 tgggggagtc gttttacccg ccgccggccg ggcctcgtcg tctgattggc tctcggggcc
      541 cagaaaactg gcccttgcca ttggctcgtg ttcgtgcaag ttgagtccat ccgccggcca
      601 gcgggggcgg cgaggaggcg ctcccaggtt ccggccctcc cctcggcccc gcgccgcaga
      661 gtctggccgc gcgcccctgc gcaacgtggc aggaagcgcg cgctgggggc ggggacgggc
      721 agtagggctg agcggctgcg gggcgggtgc aagcacgttt ccgacttgag ttgcctcaag
      781 aggggcgtgc tgagccagac ctccatcgcg cactccgggg agtggaggga aggagcgagg
      841 gctcagttgg gctgttttgg aggcaggaag cacttgctct cccaaagtcg ctctgagttg
      901 ttatcagtaa gggagctgca gtggagtagg cggggagaag gccgcaccct tctccggagg
      961 ggggagggga gtgttgcaat agctcactgg gagttctctg ctgcctcctg gcttctgagg
     1021 acagtactgg gcctgggaga atcccttgcc ccctcttccc tcacgagtga tctgcaactc
     1081 cagtctttct agtttaaacg catctgtagg gcgcagtagt ccagggtttc cttgatgatg
     1141 tcatacttat cctgtccctt ttttttccac agctcgcggt tgaggacaaa ctcttcgcgg
     1201 tctttccagt ggttaattaa gtcgacattg attattgact agttattaat agtaatcaat
     1261 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     1321 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     1381 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     1441 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     1501 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     1561 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac
     1621 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat
     1681 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggsgsg cgcsrggsgg
     1741 ggsggggsgg ggsgrggggs ggggsggggs grggsggrgr ggkgcggcgg cagccaatca
     1801 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
     1861 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc
     1921 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
     1981 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt
     2041 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg
     2101 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc
     2161 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt
     2221 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa
     2281 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt
     2341 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
     2401 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
     2461 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg grgggctcgg gggaggggcg
     2521 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt
     2581 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa
     2641 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg
     2701 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
     2761 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc
     2821 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
     2881 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
     2941 tggcaaagaa aatataactt cgtataatgt atgctatacg aagttattcg cgatgaataa
     3001 atgaaagctt gcagatctgc gactctagag gatctgcgac tctagaggat cataatcagc
     3061 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     3121 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     3181 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3241 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg cgactctaga
     3301 ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     3361 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     3421 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3481 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     3541 tctgcgactc tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt
     3601 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     3661 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3721 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3781 ttatcatgtc tggatcccca tcaagctgat ccggaaccct taatataact tcgtataatg
     3841 tatgctatac gaagttatta ggtccctcga cctgcagccc aagctagatc gaattcggcc
     3901 ggccttcgaa cacgatcagt gtgagggagt gtaaagctgg tttgccacca tggacaagaa
     3961 gtactccatt gggctcgcta tcggcacaaa cagcgtcggc tgggccgtca ttacggacga
     4021 gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc acagcataaa
     4081 gaagaacctc attggcgccc tcctgttcga ctccggggag acggccgaag ccacgcggct
     4141 caaaagaaca gcacggcgca gatatacccg cagaaagaat cggatctgct acctgcagga
     4201 gatctttagt aatgagatgg ctaaggtgga tgactctttc ttccataggc tggaggagtc
     4261 ctttttggtg gaggaggata aaaagcacga gcgccaccca atctttggca atatcgtgga
     4321 cgaggtggcg taccatgaaa agtacccaac catatatcat ctgaggaaga agcttgtaga
     4381 cagtactgat aaggctgact tgcggttgat ctatctcgcg ctggcgcata tgatcaaatt
     4441 tcggggacac ttcctcatcg agggggacct gaacccagac aacagcgatg tcgacaaact
     4501 ctttatccaa ctggttcaga cttacaatca gcttttcgaa gagaacccga tcaacgcatc
     4561 cggagttgac gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc ggctcgaaaa
     4621 cctcatcgca cagctccctg gggagaagaa gaacggcctg tttggtaatc ttatcgccct
     4681 gtcactcggg ctgaccccca actttaaatc taacttcgac ctggccgaag atgccaagct
     4741 tcaactgagc aaagacacct acgatgatga tctcgacaat ctgctggccc agatcggcga
     4801 ccagtacgca gacctttttt tggcggcaaa gaacctgtca gacgccattc tgctgagtga
     4861 tattctgcga gtgaacacgg agatcaccaa agctccgctg agcgctagta tgatcaagcg
     4921 ctatgatgag caccaccaag acttgacttt gctgaaggcc cttgtcagac agcaactgcc
     4981 tgagaagtac aaggaaattt tcttcgatca gtctaaaaat ggctacgccg gatacattga
     5041 cggcggagca agccaggagg aattttacaa atttattaag cccatcttgg aaaaaatgga
     5101 cggcaccgag gagctgctgg taaagcttaa cagagaagat ctgttgcgca aacagcgcac
     5161 tttcgacaat ggaagcatcc cccaccagat tcacctgggc gaactgcacg ctatcctcag
     5221 gcggcaagag gatttctacc cctttttgaa agataacagg gaaaagattg agaaaatcct
     5281 cacatttcgg ataccctact atgtaggccc cctcgcccgg ggaaattcca gattcgcgtg
     5341 gatgactcgc aaatcagaag agaccatcac tccctggaac ttcgaggaag tcgtggataa
     5401 gggggcctct gcccagtcct tcatcgaaag gatgactaac tttgataaaa atctgcctaa
     5461 cgaaaaggtg cttcctaaac actctctgct gtacgagtac ttcacagttt ataacgagct
     5521 caccaaggtc aaatacgtca cagaagggat gagaaagcca gcattcctgt ctggagagca
     5581 gaagaaagct atcgtggacc tcctcttcaa gacgaaccgg aaagttaccg tgaaacagct
     5641 caaagaagac tatttcaaaa agattgaatg tttcgactct gttgaaatca gcggagtgga
     5701 ggatcgcttc aacgcatccc tgggaacgta tcacgatctc ctgaaaatca ttaaagacaa
     5761 ggacttcctg gacaatgagg agaacgagga cattcttgag gacattgtcc tcacccttac
     5821 gttgtttgaa gatagggaga tgattgaaga acgcttgaaa acttacgctc atctcttcga
     5881 cgacaaagtc atgaaacagc tcaagaggcg ccgatataca ggatgggggc ggctgtcaag
     5941 aaaactgatc aatgggatcc gagacaagca gagtggaaag acaatcctgg attttcttaa
     6001 gtccgatgga tttgccaacc ggaacttcat gcagttgatc catgatgact ctctcacctt
     6061 taaggaggac atccagaaag cacaagtttc tggccagggg gacagtcttc acgagcacat
     6121 cgctaatctt gcaggtagcc cagctatcaa aaagggaata ctgcagaccg ttaaggtcgt
     6181 ggatgaactc gtcaaagtaa tgggaaggca taagcccgag aatatcgtta tcgagatggc
     6241 ccgagagaac caaactaccc agaagggaca gaagaacagt agggaaagga tgaagaggat
     6301 tgaagagggt ataaaagaac tggggtccca aatccttaag gaacacccag ttgaaaacac
     6361 ccagcttcag aatgagaagc tctacctgta ctacctgcag aacggcaggg acatgtacgt
     6421 ggatcaggaa ctggacatca atcggctctc cgactacgac gtggctgcta tcgtgcccca
     6481 gtcttttctc aaagatgatt ctattgataa taaagtgttg acaagatccg ataaagctag
     6541 agggaagagt gataacgtcc cctcagaaga agttgtcaag aaaatgaaaa attattggcg
     6601 gcagctgctg aacgccaaac tgatcacaca acggaagttc gataatctga ctaaggctga
     6661 acgaggtggc ctgtctgagt tggataaagc cggcttcatc aaaaggcagc ttgttgagac
     6721 acgccagatc accaagcacg tggcccaaat tctcgattca cgcatgaaca ccaagtacga
     6781 tgaaaatgac aaactgattc gagaggtgaa agttattact ctgaagtcta agctggtctc
     6841 agatttcaga aaggactttc agttttataa ggtgagagag atcaacaatt accaccatgc
     6901 gcatgatgcc tacctgaatg cagtggtagg cactgcactt atcaaaaaat atcccaagct
     6961 tgaatctgaa tttgtttacg gagactataa agtgtacgat gttaggaaaa tgatcgcaaa
     7021 gtctgagcag gaaataggca aggccaccgc taagtacttc ttttacagca atattatgaa
     7081 ttttttcaag accgagatta cactggccaa tggagagatt cggaagcgac cacttatcga
     7141 aacaaacgga gaaacaggag aaatcgtgtg ggacaagggt agggatttcg cgacagtccg
     7201 gaaggtcctg tccatgccgc aggtgaacat cgttaaaaag accgaagtac agaccggagg
     7261 cttctccaag gaaagtatcc tcccgaaaag gaacagcgac aagctgatcg cacgcaaaaa
     7321 agattgggac cccaagaaat acggcggatt cgattctcct acagtcgctt acagtgtact
     7381 ggttgtggcc aaagtggaga aagggaagtc taaaaaactc aaaagcgtca aggaactgct
     7441 gggcatcaca atcatggagc gatcaagctt cgaaaaaaac cccatcgact ttctcgaggc
     7501 gaaaggatat aaagaggtca aaaaagacct catcattaag cttcccaagt actctctctt
     7561 tgagcttgaa aacggccgga aacgaatgct cgctagtgcg ggcgagctgc agaaaggtaa
     7621 cgagctggca ctgccctcta aatacgttaa tttcttgtat ctggccagcc actatgaaaa
     7681 gctcaaaggg tctcccgaag ataatgagca gaagcagctg ttcgtggaac aacacaaaca
     7741 ctaccttgat gagatcatcg agcaaataag cgaattctcc aaaagagtga tcctcgccga
     7801 cgctaacctc gataaggtgc tttctgctta caataagcac agggataagc ccatcaggga
     7861 gcaggcagaa aacattatcc acttgtttac tctgaccaac ttgggcgcgc ctgcagcctt
     7921 caagtacttc gacaccacca tagacagaaa gcggtacacc tctacaaagg aggtcctgga
     7981 cgccacactg attcatcagt caattacggg gctctatgaa acaagaatcg acctctctca
     8041 gctcggtgga gacagcaggg ctgaccccaa gaagaagagg aaggtgtcgc cagggatccg
     8101 tcgacttgac gcgttgatat caacaagttt gtacaaaaaa gcaggctaca aagaggccag
     8161 cggttccgga cgggctgacg cattggacga ttttgatctg gatatgctgg gaagtgacgc
     8221 cctcgatgat tttgaccttg acatgcttgg ttcggatgcc cttgatgact ttgacctcga
     8281 catgctcggc agtgacgccc ttgatgattt cgacctggac atgctgatta actctagaag
     8341 ttccggatct ccgaaaaaga aacgcaaagt tggtagccag tacctgcccg acaccgacga
     8401 ccggcaccgg atcgaggaaa agcggaagcg gacctacgag acattcaaga gcatcatgaa
     8461 gaagtccccc ttcagcggcc ccaccgaccc tagacctcca cctagaagaa tcgccgtgcc
     8521 cagcagatcc agcgccagcg tgccaaaacc tgccccccag ccttacccct tcaccagcag
     8581 cctgagcacc atcaactacg acgagttccc taccatggtg ttccccagcg gccagatctc
     8641 tcaggcctct gctctggctc cagcccctcc tcaggtgctg cctcaggctc ctgctcctgc
     8701 accagctcca gccatggtgt ctgcactggc tcaggcacca gcacccgtgc ctgtgctggc
     8761 tcctggacct ccacaggctg tggctccacc agcccctaaa cctacacagg ccggcgaggg
     8821 cacactgtct gaagctctgc tgcagctgca gttcgacgac gaggatctgg gagccctgct
     8881 gggaaacagc accgatcctg ccgtgttcac cgacctggcc agcgtggaca acagcgagtt
     8941 ccagcagctg ctgaaccagg gcatccctgt ggcccctcac accaccgagc ccatgctgat
     9001 ggaatacccc gaggccatca cccggctcgt gacaggcgct cagaggcctc ctgatccagc
     9061 tcctgcccct ctgggagcac caggcctgcc taatggactg ctgtctggcg acgaggactt
     9121 cagctctatc gccgatatgg atttctcagc cttgctgggc tctggcagcg gcagccggga
     9181 ttccagggaa gggatgtttt tgccgaagcc tgaggccggc tccgctatta gtgacgtgtt
     9241 tgagggccgc gaggtgtgcc agccaaaacg aatccggcca tttcatcctc caggaagtcc
     9301 atgggccaac cgcccactcc ccgccagcct cgcaccaaca ccaaccggtc cagtacatga
     9361 gccagtcggg tcactgaccc cggcaccagt ccctcagcca ctggatccag cgcccgcagt
     9421 gactcccgag gccagtcacc tgttggagga tcccgatgaa gagacgagcc aggctgtcaa
     9481 agcccttcgg gagatggccg atactgtgat tccccagaag gaagaggctg caatctgtgg
     9541 ccaaatggac ctttcccatc cgcccccaag gggccatctg gatgagctga caaccacact
     9601 tgagtccatg accgaggatc tgaacctgga ctcacccctg accccggaat tgaacgagat
     9661 tctggatacc ttcctgaacg acgagtgcct cttgcatgcc atgcatatca gcacaggact
     9721 gtccatcttc gacacatctc tgtttggatc cggcgcaaca aacttctctc tgctgaaaca
     9781 agccggagat gtcgaagaga atcctggacc gatggtgagc aagggcgagg aggataacat
     9841 ggcctctctc ccagcgacac atgagttaca catctttggc tccatcaacg gtgtggactt
     9901 tgacatggtg ggtcagggca ccggcaatcc aaatgatggt tatgaggagt taaacctgaa
     9961 gtccaccaag ggtgacctcc agttctcccc ctggattctg gtccctcata tcgggtatgg
    10021 cttccatcag tacctgccct accctgacgg gatgtcgcct ttccaggccg ccatggtaga
    10081 tggctccgga taccaagtcc atcgcacaat gcagtttgaa gatggtgcct cccttactgt
    10141 taactaccgc tacacctacg agggaagcca catcaaagga gaggcccagg tgaaggggac
    10201 tggtttccct gctgacggtc ctgtgatgac caactcgctg accgctgcgg actggtgcag
    10261 gtcgaagaag acttacccca acgacaaaac catcatcagt acctttaagt ggagttacac
    10321 cactggaaat ggcaagcgct accggagcac tgcgcggacc acctacacct ttgccaagcc
    10381 aatggcggct aactatctga agaaccagcc gatgtacgtg ttccgtaaga cggagctcaa
    10441 gcactccaag accgagctca acttcaagga gtggcaaaag gcctttaccg atgtgatggg
    10501 catggacgag ctgtacaagt aaaaacgttg ttgtttgggg ttgaattact ctaatcaacc
    10561 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
    10621 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
    10681 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
    10741 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
    10801 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
    10861 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
    10921 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
    10981 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
    11041 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
    11101 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
    11161 acctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
    11221 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
    11281 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
    11341 gaggattggg aagacaatgg caggcatgct ggggaggcta gagcggccgc cgcggatatc
    11401 gaattcgcta gaagatgggc gggagtcttc tgggcaggct taaaggctaa cctggtgtgt
    11461 gggcgttgtc ctgcagggga attgaacagg tgtaaaattg gagggacaag acttcccaca
    11521 gattttcggt tttgtcggga agttttttaa taggggcaaa taaggaaaat gggaggatag
    11581 gtagtcatct ggggttttat gcagcaaaac tacaggttat tattgcttgt gatccgcctc
    11641 ggagtatttt ccatcgaggt agattaaaga catgctcacc cgagttttat actctcctgc
    11701 ttgagatcct tactacagta tgaaattaca gtgtcgcgag ttagactatg taagcagaat
    11761 tttaatcatt tttaaagagc ccagtacttc atatccattt ctcccgctcc ttctgcagcc
    11821 ttatcaaaag gtattttaga acactcattt tagccccatt ttcatttatt atactggctt
    11881 atccaacccc tagacagagc attggcattt tccctttcct gatcttagaa gtctgatgac
    11941 tcatgaaacc agacagatta gttacataca ccacaaatcg aggctgtagc tggggcctca
    12001 acactgcagt tcttttataa ctccttagta cactttttgt tgatcctttg ccttgatcct
    12061 taattttcag tgtctatcac ctctcccgtc aggtggtgtt ccacatttgg gcctattctc
    12121 agtccaggga gttttacaac aatagatgta ttgagaatcc aacctaaagc ttaactttcc
    12181 actcccatga atgcctctct cctttttctc catttataaa ctgagctatt aaccattaat
    12241 ggtttccagg tggatgtctc ctcccccaat attacctgat gtatcttaca tattgccagg
    12301 ctgatatttt aagacattaa aaggtatatt tcattattga gccacatggt attgattact
    12361 gcttactaaa attttgtcat tgtacacatc tgtaaaaggt ggttcctttt ggaatgcaaa
    12421 gttcaggtgt ttgttgtctt tcctgaccta aggtcttgtg agcttgtatt ttttctattt
    12481 aagcagtgct ttctcttgga ctggcttgac tcatggcatt ctacacgtta ttgctggtct
    12541 aaatgtgatt ttgccaagct tcttcaggac ctataatttt gcttgacttg tagccaaaca
    12601 caagtaaaat gattaagcaa caaatgtatt tgtgaagctt ggtttttagg ttgttgtgtt
    12661 gtgtgtgctt gtgctctata ataatactat ccaggggctg gagaggtggc tcggagttca
    12721 agagcacaga ctgctcttcc agaagtcctg agttcaattc ccagcaacca catggtggct
    12781 cacaaccatc tgtaatggga tctgatgccc tcttctggtg tgtctgaaga ccacaagtgt
    12841 attcacatta aataaataaa tcctccttct tcttcttttt ttttttttta aagagaatac
    12901 tgtctccagt agaatttact gaagtaatga aatactttgt gtttgttcca atatggtagc
    12961 caataatcaa attactcttt aagcactgga aatgttacca aggaactaat ttttatttga
    13021 agtgtaactg tggacagagg agccataact gcagacttgt gggatacaga agaccaatgc
    13081 agactttaat gtcttttctc ttacactaag caataaagaa ataaaaattg aacttctagt
    13141 atcctatttg tttaaactgc tagctttact taacttttgt gcttcatcta tacaaagctg
    13201 aaagctaagt ctgcagccat tactaaacat gaaagcaagt aatgataatt ttggatttca
    13261 aaaatgtagg gccagagttt agccagccag tggtggtgct tgcctttatg cctttaatcc
    13321 cagcactctg gaggcagaga caggcagatc tctgagtttg agcccagcct ggtctacaca
    13381 tcaagttcta tctaggatag ccaggaatac acacagaaac cctgttgggg aggggggctc
    13441 tgagatttca taaaattata attgaagcat tccctaatga gccactatgg atgtggctaa
    13501 atccgtctac ctttctgatg agatttgggt attatttttt ctgtctctgc tgttggttgg
    13561 gtcttttgac actgtgggct ttctttaaag cctccttcct gccatgtggt ctcttgtttg
    13621 ctactaactt cccatggctt aaatggcatg gctttttgcc ttctaagggc agctgctgag
    13681 atttgcagcc tgatttccag ggtggggttg ggaaatcttt caaacactaa aattgtcctt
    13741 taattttttt tttaaaaaat gggttatata ataaacctca taaaatagtt atgaggagtg
    13801 aggtggacta atattaaatg agtccctccc ctataaaaga gctattaagg ctttttgtct
    13861 tatacttaac ttttttttta aatgtggtat ctttagaacc aagggtctta gagttttagt
    13921 atacagaaac tgttgcatcg cttaatcaga ttttctagtt tcaaatccag agaatccaaa
    13981 ttcttcacag ccaaagtcaa attaagaatt tctgactttt aatgttaatt tgcttactgt
    14041 gaatataaaa atgatagctt ttcctgaggc agggtctcac tatgtatctc tgcctgatct
    14101 gcaacaagat atgtagacta aagttctgcc tgcttttgtc tcctgaatac taaggttaaa
    14161 atgtagtaat acttttggaa cttgcaggtc agattctttt ataggggaca cactaaggga
    14221 gcttgggtga tagttggtaa aatgtgtttc aagtgatgaa aacttgaatt attatcaccg
    14281 caacctactt tttaaaaaaa aaagccaggc ctgttagagc atgcttaagg gatccctagg
    14341 acttgctgag cacacaagag tagttacttg gcaggctcct ggtgagagca tatttcaaaa
    14401 aacaaggcag acaaccaaga aactacagtt aaggttacct gtctttaaac catctgcata
    14461 tacacaggga tattaaaata ttccaaataa tatttcattc aagttttccc ccatcaaatt
    14521 gggacatgga tttctccggt gaataggcag agttggaaac taaacaaatg ttggttttgt
    14581 gatttgtgaa attgttttca agtgatagtt aaagcccatg agatacagaa caaagctgct
    14641 atttcgaggt ctcttggttt atactcagaa gcacttcttt gggtttccct gcactatcct
    14701 gatcatgtgc taggcctacc ttaggctgat tgttgttcaa ataaacttaa gtttcctgtc
    14761 aggtgatgtc atatgatttc atatatcaag gcaaaacatg ttatatatgt taaacatttg
    14821 tacttaatgt gaaagttagg tctttgtggg tttgattttt aattttcaaa acctgagcta
    14881 aataagtcat ttttacatgt cttacatttg gtggaattgt ataattgtgg tttgcaggca
    14941 agactctctg acctagtaac cctacctata gagcactttg ctgggtcaca agtctaggag
    15001 tcaagcattt caccttgaag ttgagacgtt ttgttagtgt atactagttt atatgttgga
    15061 ggacatgttt atccagaaga tattcaggac tatttttgac tgggctaagg aattgattct
    15121 gattagcact gttagtgagc attgagtggc ctttaggctt gaattggagt cacttgtata
    15181 tctcaaataa tgctggcctt ttttaaaaag cccttgttct ttatcaccct gttttctaca
    15241 taatttttgt tcaaagaaat acttgtttgg atctcctttt gacaacaata gcatgttttc
    15301 aagccatatt ttttttcctt tttttttttt tttttggttt ttcgagacag ggtttctctg
    15361 tatagccctg gctgtcctgg aactcacttt gtagaccagg ctggcctcga actcagaaat
    15421 ccgcctgcct ctgcctcctg agtgccggga ttaaaggcgt gcaccaccac gcctggctaa
    15481 gttggatatt ttgttatata actataacca atactaactc cactgggtgg atttttaatt
    15541 cagtcagtag tcttaagtgg tctttattgg cccttcatta aaatctactg ttcactctaa
    15601 cagaggctgt tggtactagt ggcacttaag caacttccta cggatatact agcagattaa
    15661 gggtcaggga tagaaactag tctagcgttt tgtataccta ccagctttat actaccttgt
    15721 tctgatagaa atatttcagg acatctagct tatcgatacc gtcgacggta tcgataagct
    15781 tgatatcgaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
    15841 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
    15901 cacatccacc ggatgcattc cactgacaga agacactgcc cctgaataca agcaggtttc
    15961 tgatgggtgg agaggcaaag gcagtgtcgt caaggctcta gggaaggagc aggtcagtct
    16021 attcagtaca aacaagtttt catgcgcctt acaaaaacca tcctaacaca gacaccagtc
    16081 ccaccatcgg tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
    16141 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    16201 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    16261 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    16321 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    16381 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    16441 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    16501 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    16561 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    16621 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    16681 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    16741 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    16801 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    16861 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    16921 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    16981 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    17041 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    17101 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    17161 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    17221 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    17281 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    17341 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    17401 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    17461 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    17521 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    17581 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    17641 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    17701 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    17761 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    17821 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    17881 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    17941 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    18001 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    18061 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    18121 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    18181 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    18241 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaamagt gcmacctnnn
    18301 nnnnnnnnnn raattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
    18361 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
    18421 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
    18481 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
    18541 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
    18601 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
    18661 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
    18721 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcrmktycca ttcgccattc
    18781 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    18841 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    18901 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
    18961 gagct
//
