LOCUS       sequence-384452-       10143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 S_Q52R in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 S_Q52R
FEATURES             Location/Qualifiers
     source          1..10143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(319..1110,1111..1179))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     942..961
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1180..1284)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1359..1378)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1550..1929
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1930..2133
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2084..2104
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2135..2153
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2156..2174
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2184..2208
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2351..2375
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6195..6219)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6266..6289
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6326..6349
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6380..6406
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             6380..6406
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(6407..6754,6755..6757,6758..7366)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(7410..7427)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7416..7640
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7773..7792)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7924..7971
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7979..9001
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7979..9001
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9131..9252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9168..9187)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9222..9241
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9301..9317)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9301..9317)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9314..9336)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9325..9341
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9349..9355,9356..9373,9374..9379))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9394..9415
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9532..9549)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9703..10143,1..148))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9783..9802)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
       61 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
      121 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
      181 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
      241 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
      301 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
      361 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
      421 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
      481 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
      541 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
      601 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
      661 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
      721 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
      781 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
      841 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
      901 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
      961 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     1021 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     1081 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     1141 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     1201 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     1261 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     1321 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     1381 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     1441 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     1501 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
     1561 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     1621 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     1681 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     1741 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     1801 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     1861 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     1921 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     1981 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     2041 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     2101 cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag agatctccct
     2161 atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     2221 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta
     2281 gcgtttaaac ttaagcttgg taccgagctc ggatccacta gtccagtgtg gtggaattct
     2341 gcagatatca acaagtttgt acaaaaaagt tggcatgttc gtgttcctgg tgctgctgcc
     2401 actggtgagc tcccagtgcg tgaacctgac caccagaacc cagctgccac ctgcctacac
     2461 caactccttc accaggggcg tgtactaccc agacaaggtg ttcagatcca gcgtgctgca
     2521 ctctaccagg gacctgttcc tgccattctt ctctaacgtg acctggttcc acgccatcca
     2581 cgtgtctgga accaacggca ccaagagatt cgacaaccca gtgctgccat tcaacgacgg
     2641 cgtgtacttc gcttctaccg agaagtctaa catcatcagg ggttggatct tcggaaccac
     2701 cctggactct aagacccagt ctctgctgat cgtgaacaac gctaccaacg tggtgatcaa
     2761 ggtgtgcgag ttccagttct gcaacgaccc attcctggga gtgtactacc acaagaacaa
     2821 caagtcttgg atggaaagcg agttcagggt gtacagctct gctaacaact gcaccttcga
     2881 gtacgtgtct cagcctttcc tgatggacct ggaaggaaag cagggaaact tcaagaacct
     2941 gagagagttc gtgttcaaga acatcgacgg ttacttcaag atatacagca agcacacccc
     3001 aatcaacctg gtgagggacc tgccacaggg tttctctgct ctggagcccc tggtggacct
     3061 gccaatcggc atcaacatca ccaggttcca gaccctgctg gctctgcaca ggtcttacct
     3121 gaccccaggc gacagcagct ctggatggac cgccggcgcc gctgcttact acgtgggata
     3181 cctgcagcca aggaccttcc tgctgaagta caacgaaaac ggcaccatca ccgacgccgt
     3241 ggactgcgct ctggacccac tgtctgagac caagtgcacc ctgaagtctt tcaccgtgga
     3301 aaagggaatc taccagacca gcaacttccg cgtgcagccc accgagtcta tcgtgagatt
     3361 cccaaacatc accaacctgt gcccattcgg agaagtgttc aacgctacca ggttcgccag
     3421 cgtgtacgct tggaacagga agagaatcag caactgcgtg gccgactact ctgtgctgta
     3481 caactctgcc tccttctcca ccttcaagtg ctacggcgtg tccccaacca agctgaacga
     3541 cctgtgcttc accaacgtgt acgctgactc cttcgtgatc agaggagacg aagtgagaca
     3601 gatcgctccc ggtcagaccg gaaagatcgc cgactacaac tacaagctgc cagacgactt
     3661 caccggttgc gtgatcgctt ggaactctaa caacctggac tctaaggtgg gaggcaacta
     3721 caactacctg tacaggctgt tccgcaagag caacctgaag cctttcgaaa gggacatcag
     3781 caccgaaatc taccaggctg gatctacccc atgcaacgga gtggaaggat tcaactgcta
     3841 cttccctctg cagtcttacg gattccagcc aaccaacgga gtgggatacc agccatacag
     3901 agtggtggtg ctgtctttcg agctgctgca cgctcctgct accgtgtgcg gaccaaagaa
     3961 gtctaccaac ctggtgaaga acaagtgcgt gaacttcaac ttcaacggac tgaccggaac
     4021 cggagtgctg accgaatcca acaagaagtt cctgcctttc cagcagttcg gtagagacat
     4081 cgccgacacc accgacgctg tgagagaccc acagaccctg gaaatcctgg acatcacccc
     4141 ctgctctttc ggaggagtga gcgtgatcac cccaggaacc aacacctcca accaggtggc
     4201 cgtgctgtac caggacgtga actgcaccga agtgccagtg gccatccacg ctgaccagct
     4261 gaccccaacc tggagagtgt acagcaccgg atctaacgtg ttccagaccc gtgccggctg
     4321 cctgatcgga gccgagcacg tgaacaactc ctacgaatgc gacatcccaa tcggagctgg
     4381 catctgcgct tcttaccaga cccagaccaa cagcccaaga agggccagat ccgtggcctc
     4441 tcagagcatc atcgcctaca ccatgagcct gggcgctgaa aacagcgtgg cttacagcaa
     4501 caactctatc gccatcccaa ccaacttcac catctctgtg accaccgaaa tcctgccagt
     4561 gagcatgacc aagaccagcg tggactgcac catgtacatc tgcggagact ctaccgaatg
     4621 ctctaacctg ctgctgcagt acggcagctt ctgcacccag ctgaaccgcg ctctgaccgg
     4681 aatcgctgtg gagcaggaca agaacaccca ggaagtgttc gctcaggtga agcagatata
     4741 caagacccca cctatcaagg acttcggagg attcaacttc tctcagatcc tgcccgaccc
     4801 atctaagccc tctaagaggt ctttcatcga agacctgctg ttcaacaagg tgaccctggc
     4861 cgacgccggt ttcatcaagc agtacggtga ctgcctggga gacatcgccg ctagagacct
     4921 gatctgcgcc cagaagttca acggtctgac cgtgctgcca ccactgctga ccgacgagat
     4981 gatcgcccag tacacctctg ccctgctggc tggaaccatc acctctggtt ggaccttcgg
     5041 agctggcgcc gccctgcaga tcccattcgc tatgcagatg gcctaccgtt tcaacggtat
     5101 cggagtgacc cagaacgtgc tgtacgaaaa ccagaagctg atcgctaacc agttcaactc
     5161 tgctatcgga aagatccagg actccctgtc ctctaccgct tctgccctgg gaaagctgca
     5221 ggacgtggtg aaccagaacg cccaggccct gaacaccctg gtgaagcagc tgtcttctaa
     5281 cttcggagcc atctcctctg tgctgaacga catcctgtct aggctggaca aggtggaagc
     5341 cgaggtgcag atcgacagac tgatcaccgg aagactgcag tctctgcaga cctacgtgac
     5401 ccagcagctg atcagggccg ctgaaatcag agcttctgcc aacctggctg ccaccaagat
     5461 gtccgaatgc gtgctgggcc agtctaagag ggtggacttc tgcggaaagg gataccacct
     5521 gatgtccttc ccacagtctg ctccacacgg agtggtgttc ctgcacgtga cctacgtgcc
     5581 cgcccaggaa aagaacttca ccaccgcccc agctatctgc cacgacggaa aggctcactt
     5641 ccccagggag ggagtgttcg tgtctaacgg aacccactgg ttcgtgaccc agaggaactt
     5701 ctacgagcca cagatcatca ccaccgacaa caccttcgtg tctggaaact gcgacgtggt
     5761 gatcggaatc gtgaacaaca ccgtgtacga cccactgcag ccagaactgg actctttcaa
     5821 ggaggaactg gacaagtact tcaagaacca cacctctcca gacgtggacc tgggagacat
     5881 ctccggaatc aacgcctctg tggtgaacat ccagaaggaa atcgacagac tgaacgaggt
     5941 ggccaagaac ctgaacgaat ctctgatcga cctgcaggaa ctgggaaagt acgagcagta
     6001 catcaagtgg ccctggtaca tctggctggg tttcatcgct ggtctgatcg ctatcgtgat
     6061 ggtgaccatc atgctgtgct gcatgacctc ttgctgctct tgcctgaagg gatgctgctc
     6121 ttgcggatct tgctgcaagt tcgacgagga cgactccgag cctgtgctga agggtgtgaa
     6181 gctgcactac acctacccaa ctttcttgta caaagtggtt gatatcaagg ccgccgacat
     6241 caccagcctg tacaagaaag tggggtggag ccatccccag ttcgagaagg gcggaggatc
     6301 tggcggcgga agcggcggag gaagctggtc ccaccctcag tttgagaaag gcaccgagct
     6361 gggcagcacc atggccagct acccctacga cgtgcccgac tacgccaagg acaacaccgt
     6421 gcccctgaag ctgatcgccc tgctggccaa cggcgagttc cactctggcg agcagctggg
     6481 cgagacactg ggcatgagca gagccgccat caacaagcac atccagaccc tgcgggactg
     6541 gggcgtggac gtgttcacag tgcccggcaa gggctacagc ctgcccgagc ccatccagct
     6601 gctgaacgcc aagcagatcc tgggccagct ggatggcgga tctgtggccg tgctgcccgt
     6661 gatcgacagc accaaccagt atctgctcga ccggatcggc gagctgaagt ccggcgacgc
     6721 ctgtatcgcc gagtaccagc aggccggcag aggcggcagg ggcagaaagt ggttcagccc
     6781 cttcggcgcc aacctgtacc tgagcatgtt ctggagactg gaacagggcc cagccgccgc
     6841 tattggcctg agcctggtca tcggcatcgt gatggccgag gtgctgcgga agctgggcgc
     6901 cgacaaagtg cgcgtgaagt ggcccaacga cctgtatctg caggaccgga agctggccgg
     6961 catcctggtg gaactgaccg gcaaaacagg cgacgccgcc cagatcgtga tcggcgctgg
     7021 catcaacatg gccatgcggc gggtggaaga gagcgtggtc aaccagggct ggatcaccct
     7081 gcaggaagcc ggcatcaacc tggaccggaa caccctggcc gccatgctga tcagagagct
     7141 gagagccgcc ctggaactgt tcgagcagga aggcctggcc ccctacctga gcagatggga
     7201 gaagctggac aacttcatca accggcccgt gaaactgatc atcggcgaca aagagatctt
     7261 cggcatcagc cggggcatcg acaagcaggg ggccctgctc ctggaacagg acggcatcat
     7321 caagccctgg atgggcggcg agatcagcct gcggagcgcc gagaagtgat gactcgagtc
     7381 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     7441 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     7501 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     7561 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     7621 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     7681 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     7741 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     7801 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     7861 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtac
     7921 ctagaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt ccttggccaa
     7981 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     8041 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     8101 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     8161 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     8221 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
     8281 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
     8341 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     8401 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     8461 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     8521 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     8581 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     8641 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     8701 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
     8761 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     8821 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     8881 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     8941 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata
     9001 gcacgtacta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     9061 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     9121 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     9181 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     9241 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     9301 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9361 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9421 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9481 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9541 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9601 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9661 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9721 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9781 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9841 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9901 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9961 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10021 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10081 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10141 aag
//
