LOCUS       sequence-384457-        2541 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector containing gfasPurple.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, 
            Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
  TITLE     Komagataeibacter Tool Kit (KTK): A Modular Cloning System for 
            Multigene Constructs and Programmed Protein Secretion from Cellulose
            Producing Bacteria.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 
            10.1021/acssynbio.1c00358. Epub 2021 Nov 12.
  PUBMED    34767345
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00358"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3422-3434"
COMMENT     Sequence Label: KTK_346
FEATURES             Location/Qualifiers
     source          1..2541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     417..436
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(655..759)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      862..1450
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1351..1370
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2335..2541,1..585,586..654))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
       61 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
      121 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
      181 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
      241 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
      301 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
      361 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
      421 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
      481 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
      541 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
      601 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
      661 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
      721 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
      781 acctgtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
      841 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
      901 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
      961 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1021 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1081 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1141 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1201 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1261 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1321 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1381 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1441 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1501 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta gtcggtctcc
     1561 tatgtcggtg attgctaaac agatgaccta caaagtctat atgtcgggta cggtgaacgg
     1621 ccattatttt gaagttgaag gtgacggtaa aggcaagccg tatgaaggcg aacagaccgt
     1681 taaactgacc gtcacgaagg gcggtccgct gccgtttgca tgggatattc tgagtccgca
     1741 gtcccaatat ggcagcatcc cgttcacgaa atatccggaa gatatcccgg actacgtgaa
     1801 gcagtctttt ccggaaggtt acacctggga acgtatcatg aacttcgaag atggcgccgt
     1861 ctgcaccgtg agtaacgaca gctctattca aggtaattgt ttcatctacc atgtcaagtt
     1921 ctcaggtctg aacttcccgc cgaatggccc ggtgatgcag aaaaagaccc aaggctggga
     1981 accgaatacg gaacgtctgt ttgcacgcga tggtatgctg attggcaaca atttcatggc
     2041 tctgaaactg gaaggcggtg gccactatct gtgcgaattt aaaagcacct acaaggcgaa
     2101 aaagccggtt aaaatgccgg gctatcatta cgtggatcgt aaactggacg ttaccaacca
     2161 caataaggac tatacgtccg tcgaacagtg tgaaatttca atcgcgcgca aatcggtggt
     2221 tgcctaattc ttgagaccaa gattatcaaa aaggatcttc acctagatcc ttttaaatta
     2281 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     2341 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     2401 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     2461 tgcaatgata ccgcgggacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     2521 agccggaagg gccgagcgca g
//
