LOCUS       sequence-384458-        2013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector containing Terminator sequence (Bba_B0015).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, 
            Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
  TITLE     Komagataeibacter Tool Kit (KTK): A Modular Cloning System for 
            Multigene Constructs and Programmed Protein Secretion from Cellulose
            Producing Bacteria.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 
            10.1021/acssynbio.1c00358. Epub 2021 Nov 12.
  PUBMED    34767345
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00358"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3422-3434"
COMMENT     Sequence Label: KTK_005
FEATURES             Location/Qualifiers
     source          1..2013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      28..99
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      115..142
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(256..1047,1048..1116))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     879..898
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1117..1221)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1324..1912
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1813..1832
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat tctttattac caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
       61 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
      121 ccttcgggtg ggcctttctg cgtttataaa cgtgagacca agattatcaa aaaggatctt
      181 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
      241 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
      301 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
      361 cttaccatct ggccccagtg ctgcaatgat accgcgggac ccacgctcac cggctccaga
      421 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
      481 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
      541 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
      601 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
      661 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
      721 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
      781 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
      841 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
      901 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
      961 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     1021 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     1081 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     1141 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     1201 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgtca tgaccaaaat
     1261 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1321 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1381 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1441 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1501 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1561 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1621 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1681 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1741 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1801 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1861 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1921 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     1981 tgcgttatcc cctgattctg tggataaccg tag
//
