LOCUS       sequence-384464-       11600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Csm complex (RNase mut) from separate 
            promoters; GFP backbone (Used for RNA binding/tethering).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11600)
  AUTHORS   Colognori D, Trinidad M, Doudna JA
  TITLE     Precise transcript targeting by CRISPR-Csm complexes.
  JOURNAL   Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9.
  PUBMED    36690762
REFERENCE   2  (bases 1 to 11600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9."
COMMENT     Sequence Label: pDAC569
FEATURES             Location/Qualifiers
     source          1..11600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        45..248
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             288..305
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             312..335
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             342..362
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2646..2693
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        2724..2927
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2958..2967
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2967..2990
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2997..3017
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3405..3452
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        3483..3686
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3717..3726
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3726..3749
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3756..3776
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4446..4493
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        4524..4727
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      4758..4767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4767..4790
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4797..4817
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5724..5771
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        5802..6005
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      6036..6045
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6045..6068
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6075..6095
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7176..7223
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        7254..7457
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      7488..7497
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7497..7520
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7527..7547
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8286..8333
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        8364..8567
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      8598..8607
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8604..8606,8607..8609,8610..9323)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8604..8606,8607..8609,8610..9320)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8649..8670)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8910..8929)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9257..9278
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    9324..9371
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        9402..9650
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9402..9422
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9573..9592
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(9804..9822)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9890..9994
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9995..10063,10064..10855)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10213..10232)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(11026..11600,1..14)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11515..11534
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggagcctatg gaaaaacgcc agcaacgcgg cctttttctt aagcgtgatg cggttttggc
       61 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      121 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      181 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      241 gcagagctcg tttagtgaac cgtcagatct ctagagccgc caccatgcac catcaccatc
      301 accattccgg cgattacaaa gacgatgacg ataagatggc gcctaagaag aaacgcaaag
      361 tgcggggcat gaagaaagaa aagattgatc tgttttacgg agccctgctg cacgacatcg
      421 gaaaggtcat ccagcgagca accggagagc ggaagaaaca cgcacttgtg ggcgccgact
      481 ggttcgacga gatcgccgac aaccaagtca tctcggatca gatccggtac catatggcca
      541 actaccagtc tgataagctc ggcaacgatc acctggctta catcacctac attgccgaca
      601 acatcgcctc cggtgtcgac cgccggcaat ccaacgaaga gtcagacgaa gatacctccg
      661 caaagatctg ggacacctac acgaaccagg ccgacatctt taacgtgttc ggagcgcaga
      721 ccgataagcg gtacttcaag cctaccgtgc tgaatctcaa gtcgaagccc aacttcgcgt
      781 ccgccactta cgaacccttt agcaagggcg attacgctgc catcgccacc cggattaaga
      841 acgaactggc cgagttcgag ttcaaccaag tccagattga ctccctgctc aaccttttcg
      901 aggctactct ctccttcgtg ccgtcaagca ccaacactaa ggaaatcgcc gacatctccc
      961 tggccgacca ttcccgcttg actgctgcct tcgctctggc gatctacgac tacctggagg
     1021 acaagggtcg gcacaactac aaagaggacc tgttcaccaa agtgtcagcg ttctatgaag
     1081 aagaagcctt cctgctggcc tccttcgacc tgtcgggaat ccaggacttt atctacaaca
     1141 ttaacatcgc aactaacggc gcggcgaagc agctgaaggc ccggagcctc tacctggact
     1201 ttatgtccga gtacatcgcc gatagcctgc tggacaagct gggactgaac agggctaaca
     1261 tgctttacgt cggcggcgga cacgcctact tcgtcctggc caacaccgaa aagactgtgg
     1321 aaaccctggt gcagtttgag aaggatttca accagttcct gttggcaaac ttccagaccc
     1381 gcctctatgt ggcctttggc tggggttcct tcgcggccaa ggacatcatg tccgagctga
     1441 atagccccga gtcctaccgc caagtgtacc aaaaggcttc gcgcatgatc tccaaaaaga
     1501 aaatctccag atacgactac cagacactga tgctcctgaa tcgcggtgga aagtcctcag
     1561 agagagagtg cgagatttgc cactccgtgg agaacctggt gtcctaccac gaccagaaag
     1621 tctgtgacat ttgccgggga ctgtaccagt tctcgaaaga aattgcccat gaccacttca
     1681 tcattaccga aaatgagggg ctgccgattg gaccaaacgc gtgcttaaag ggcgtggcat
     1741 tcgaaaagct gtcccaagaa gcgttcagcc gggtctacgt gaagaatgac tataaggccg
     1801 gtaccgtgaa ggctacgcat gtgttcgtgg gggattacca gtgcgacgag atctacaact
     1861 acgccgccct gagcaagaac gagaacggcc taggcatcaa gagactggcc gtggtccggc
     1921 tcgacgtgga tgacttgggc gccgccttca tggccggttt cagccagcag ggaaacggac
     1981 aatactccac tctgtcaaga tcggccacat tctcccggag catgtcgctg ttcttcaaag
     2041 tgtacattaa ccagttcgcc tccgacaaga agctgagcat tatctacgcg ggcggcgatg
     2101 acgtgttcgc cattggatcg tggcaggata tcatcgcgtt cactgtggaa cttcgcgaaa
     2161 acttcatcaa gtggaccaac gggaagctca ccctctccgc ggggataggg ttgttcgccg
     2221 acaagactcc tattagcctg atggctcacc agaccgggga actggaagag gccgccaagg
     2281 gcaacgaaaa ggactccatc tcgctgttct caagcgacta cactttcaag tttgataggt
     2341 tcatcactaa cgtgtacgac gacaaactgg aacagattag atacttcttc aaccatcaag
     2401 acgagagggg aaagaacttc atctataagc ttattgagct tttgaggaac cacgaccgca
     2461 tgaatatggc acgcctcgcc tattacctca ctcgcctgga agaactgacc cgggagactg
     2521 acagggacaa gttcaagacc ttcaagaacc tgttctactc ctggtacacc aacaagaacg
     2581 ataaggaccg gaaggaagcc gagctcgcgc tcctgctgta catctacgaa atcagaaagg
     2641 attaacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcgcacaca
     2701 ttagctagcc gtcagcacac attgtgatgc ggttttggca gtacatcaat gggcgtggat
     2761 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2821 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2881 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     2941 gtcagatctc tagagccgcc accatggatt acaaagacga tgacgataag atggcgccta
     3001 agaagaaacg caaagtgcgg ggcatgacca tcctgaccga cgagaactac gtggacatcg
     3061 ccgagaaagc catcctgaag ctggaaagaa acaccagaaa tagaaagaac cctgatgcct
     3121 tcttcctgac cacatctaag ctgcggaacc tgctgagcct gacaagcacc ctgttcgacg
     3181 agagcaaggt gaaggaatac gacgccctgc tggacagaat cgcttatctg agagtgcagt
     3241 tcgtgtacca ggccggcaga gagatcgccg tgaaagatct gatcgagaag gcccagatcc
     3301 tggaagctct gaaagagatc aaggaccggg aaaccctgca gagattctgc agatacatgg
     3361 aagccctggt ggcctacttc aagttctacg gcggcaagga ctgacggcaa taaaaagaca
     3421 gaataaaacg cacggtgttg ggtcgtttgt tcagttcttt tgccttactt tcaatgcatg
     3481 cggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     3541 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     3601 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     3661 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatctct agagccgcca
     3721 ccatggatta caaagacgat gacgataaga tggcgcctaa gaagaaacgc aaagtgcggg
     3781 gcatgacctt cgccaagatc aaattcagcg cccagatccg gctggaaacc ggcctgcaca
     3841 tcggaggatc tgatgccttt gccgctatcg gcgccatcgc cagccctgtg atcaaggacc
     3901 ccatcaccaa cctgcctatc atccccggct ctagcctgaa gggcaagatg agaacactgc
     3961 tggccaaggt gtacaacgaa aaggtggccg agaagcctag cgacgacagc gacatcctga
     4021 gcagactgtt cggaaatagc aaggataagc ggttcaagat gggcagactg atcttccggg
     4081 acgccttcct gagcaacgcc gacgagctgg attctctggg cgtgcggagc tacaccgagg
     4141 tgaagttcga gaacaccatc gatagaatca ccgccgaggc caatcctaga cagatcgaga
     4201 gagccattcg gaactcaaca ttcgacttcg agctgatcta cgagatcact gatgagaatg
     4261 agaaccaggt cgaggaagat ttcaaggtga tcagagacgg cctgaagctg ctggaactgg
     4321 actacctggg cggaagcggc tccagaggct acggcaaagt ggcttttgag aacctgaaag
     4381 ccaccacagt gttcggcaac tacgacgtga aaaccctgaa cgagctgctg accgccgaag
     4441 tgtgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttctggattg
     4501 cgagaatgga ctagtagcaa actgtgatgc ggttttggca gtacatcaat gggcgtggat
     4561 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     4621 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     4681 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     4741 gtcagatctc tagagccgcc accatggatt acaaagacga tgacgataag atggcgccta
     4801 agaagaaacg caaagtgcgg ggcatgactt acaagctcta cattatgacc tttcaaaacg
     4861 cccacttcgg ttccggcact ctggactcat cgaagctgac cttctccgcg gatagaatct
     4921 tctcggcact cgtgctcgag gctctgaaga tgggaaagct cgacgccttc ttggccgagg
     4981 ccaaccagga taagttcact ctgaccgacg cgttcccatt ccaattcggt cctttcctgc
     5041 cgaaaccgat tggttacccc aagcacgacc agatcgacca gtctgtggac gtgaaggaag
     5101 tccgccgcca agcgaagctg tccaaaaagc tccagttcct ggctctggaa aacgtcgacg
     5161 actacctgaa cggagagctg tttgagaatg aggaacacgc cgtgatcgac acagtgacca
     5221 agaaccagcc ccataaagat gataatctgt accaagtggc caccactcgg ttctcgaacg
     5281 acacctccct ttacgtgatc gccaacgaat ccgatctgct gaacgaactg atgagcagcc
     5341 ttcagtactc cgggctgggc ggcaaaaggt cctcaggatt cggcagattt gagctggaca
     5401 tccagaacat tcccttggaa ctgtccgacc ggctgacgaa gaaccacagc gacaaggtca
     5461 tgtcacttac caccgccctc ccggtggacg ctgatctcga ggaagcgatg gaagatggcc
     5521 attacctgtt gaccaagtcg tccggattcg cattctccca cgccaccaac gaaaactatc
     5581 ggaagcagga cctgtacaag ttcgcctccg ggagcacctt cagcaagact ttcgagggac
     5641 agatcgtgga cgtgcgccct ctcgatttcc ctcacgccgt gctgaactac gccaagccgc
     5701 tgttctttaa gctcgaagtc taacggcaat aaaaagacag aataaaacgc acggtgttgg
     5761 gtcgtttgtt cgcacattca aaaacaggca attggacaag cgtgatgcgg ttttggcagt
     5821 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     5881 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     5941 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     6001 gagctcgttt agtgaaccgt cagatctcta gagccgccac catggattac aaagacgatg
     6061 acgataagat ggcgcctaag aagaaacgca aagtgcgggg catgaaaaat gactaccgga
     6121 ccttcaagct gagcctgctg accctggctc ctatccacat cggcaacggc gagaagtaca
     6181 ccagcagaga attcatctac gagaacaaga agttctactt ccccgacatg ggcaagttct
     6241 acaacaagat ggtggaaaag agactggccg agaagttcga ggccttcctg atccagacca
     6301 gacccaacgc cagaaacaac cggctgattt cttttctgaa cgacaacaga atcgccgaaa
     6361 gatcttttgg cggctacagc atcagtgaaa ccggcctgga atctgataag aaccctaaca
     6421 gcgccggagc tatcaacgag gtgaacaaat tcatccggga cgccttcgga aatccttaca
     6481 tcccaggcag cagcctgaag ggcgccatcc gcaccatcct gatgaacacc acacctaagt
     6541 ggaacaacga gaacgccgtg aacgacttcg gcagattccc aaaggaaaac aagaacctga
     6601 tcccttgggg acctaagaaa ggcaaggaat acgacgacct gttcaacgcc atcagagtgt
     6661 ccgacagcaa gcccttcgac aacaaaagcc tgatcctcgt gcagaagtgg gactacagcg
     6721 ccaaaaccaa caaggccaag cctctgcctc tgtacagaga gtctatcagc cctctgacca
     6781 agatcgagtt cgagataaca acaaccactg atgaggccgg cagactgatc gaggaactgg
     6841 gaaagcgggc ccaggccttt tataaggact acaaggcctt tttcctgtct gaattccctg
     6901 atgataagat ccaggctaat ctgcaatacc ccatctacct gggcgccggc agcggcgctt
     6961 ggacaaagac cctgtttaag caggccgacg gcatcctgca gcggagatac tccagaatga
     7021 aaaccaagat ggtcaagaag ggcgtgctga agctgacaaa ggcccctctg aaaacagtga
     7081 agatccccag cggcaaccac agcctggtga agaatcacga gagcttctac gagatgggca
     7141 aagccaactt catgatcaag gaaatcgaca agtgacggca ataaaaagac agaataaaac
     7201 gcacggtgtt gggtcgtttg ttcatgtgtg ccggccggag aataaaagtc taagtgatgc
     7261 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     7321 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     7381 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     7441 tctatataag cagagctcgt ttagtgaacc gtcagatctc tagagccgcc accatggatt
     7501 acaaagacga tgacgataag atggcgccta agaagaaacg caaagtgcgg ggcatgaaaa
     7561 agctcgtgtt cacctttaag cggatcgacc accctgctca ggacctggcc gtgaaattcc
     7621 acggcttcct gatggaacag ctggatagcg actacgtgga ctacctgcac cagcagcaga
     7681 ccaaccccta cgccacaaag gtgatccagg gcaaagagaa cacccagtgg gtcgtgcatc
     7741 tgctgacaga cgacatcgag gacaaggtgt tcatgaccct gctgcagatc aaggaagtgt
     7801 ccctgaacga cctgcctaag ttgtctgtgg aaaaggtgga aatccaggag ctgggcgctg
     7861 ataagctgct cgagatcttc aacagcgagg aaaaccagac ctacttcagc atcatcttcg
     7921 agacacctac aggctttaaa agccagggca gctacgtgat cttccccagc atgcggctga
     7981 tctttcagag cctgatgcag aagtacggca gactggtgga aaaccagcct gagatcgagg
     8041 aagataccct ggactacctg agcgagcaca gcaccatcac caattacaga ctggaaacaa
     8101 gctacttcag agtgcataga cagagaatcc ccgccttccg gggcaagctg accttcaagg
     8161 tgcagggagc ccagacactg aaggcctacg tgaagatgct gctgaccttc ggcgagtaca
     8221 gcggcctggg catgaaaacc agcctgggaa tgggcggcat caagctggaa gaaagaaagg
     8281 actgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcgtgcgat
     8341 agagggatcc cgcattgaat tatgtgatgc ggttttggca gtacatcaat gggcgtggat
     8401 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     8461 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     8521 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     8581 gtcagatctc tagagccgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     8641 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     8701 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     8761 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     8821 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     8881 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     8941 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     9001 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     9061 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     9121 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     9181 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     9241 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     9301 gcatggacga gctgtacaag tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     9361 gtcgtttgtt cgtagatggc gcgcctttgt tgacccggtt ggagggccta tttcccatga
     9421 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga
     9481 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
     9541 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
     9601 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc gatataaacc
     9661 taattacctc gagaggggac ggaaacccgt cttcgatgaa gcgattcaga agacttgata
     9721 taaacctaat tacctcgaga ggggactttt ttacatgtgt cagaggtttt caccgtcatc
     9781 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     9841 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     9901 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     9961 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
    10021 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
    10081 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
    10141 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    10201 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
    10261 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    10321 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    10381 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    10441 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    10501 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
    10561 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    10621 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    10681 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    10741 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    10801 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    10861 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    10921 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    10981 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    11041 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    11101 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    11161 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    11221 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    11281 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    11341 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    11401 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    11461 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    11521 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    11581 gtgatgctcg tcaggggggc
//
