LOCUS       sequence-384473-        8246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral construct of TGF-beta reporter (nuclear mCherry) and hPGK
            driven rtTA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8246)
  AUTHORS   Yuan S, Stewart KS, Yang Y, Abdusselamoglu MD, Parigi SM, Feinberg 
            TY, Tumaneng K, Yang H, Levorse JM, Polak L, Ng D, Fuchs E
  TITLE     Ras drives malignancy through stem cell crosstalk with the 
            microenvironment.
  JOURNAL   Nature. 2022 Nov 30. doi: 10.1038/s41586-022-05475-6.
  PUBMED    36450983
REFERENCE   2  (bases 1 to 8246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 Nov 30. doi: 10.1038/s41586-022-05475-6."
COMMENT     Sequence Label: SBE-NLS-mCherry PGK-rtTA3
FEATURES             Location/Qualifiers
     source          1..8246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1833..2081
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1833..1853
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2004..2023
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2177..2294
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2343..2853
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2772..2790
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2875..3579
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     CDS             complement(3635..4342)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3910..3929)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4179..4197
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(4352..4372)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     LTR             4765..4998
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5070..5191
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5107..5126)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5161..5180
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5231..5366
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5386..5405)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5387..5405)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5415..5432)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5415..5431)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5424..5446)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5573..6028
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5660..5679)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5870..5891
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6054..6158
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6159..6227,6228..7019)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6377..6396)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7190..7778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7679..7698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7932..7949
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8066..8087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8102..8107,8108..8125,8126..8132)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8140..8156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8145..8167
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8164..8180
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8164..8180
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8199..8219
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8201..8219
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     1801 gagactagcc tcgagcggcc gcccccttca ccgagggcct atttcccatg attccttcat
     1861 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca
     1921 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     1981 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     2041 atttcttggc tttatatatc ttgtggaaag gacgaaacac cggcaacaag atgaagagca
     2101 ccaactcgag ttggtgctct tcatcttgtt gtttttaatt ctcgacctcg agacaaatgg
     2161 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     2221 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     2281 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg ccgcggctcg
     2341 agggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc
     2401 tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg
     2461 tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt
     2521 cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa
     2581 acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca
     2641 gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc
     2701 agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc
     2761 ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc
     2821 ctcgttgacc gaatcaccga cctctctccc cagggggatc caccggagct taccatgtct
     2881 aggctggaca agagcaaagt cataaacgga gctctggaat tactcaatgg tgtcggtatc
     2941 gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac
     3001 tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg
     3061 catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac
     3121 gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc
     3181 acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag
     3241 caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg
     3301 ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc
     3361 accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg gcagggagcc
     3421 gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag
     3481 tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat gctcccagcc
     3541 gatgcccttg acgattttga ccttgacatg ctccccgggg aaatgcatgt ttaaacgact
     3601 gctagcgact tctagattat gctagctcgg ccgccttgta cagctcgtcc atgccgccgg
     3661 tggagtggcg gccctcggcg cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg
     3721 aggtgatgtc caacttgatg ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg
     3781 ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct
     3841 gcttgatctc gcccttcagg gcgccgtcct cggggtacat ccgctcggag gaggcctccc
     3901 agcccatggt cttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct
     3961 tcaccttgta gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc
     4021 cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca
     4081 agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac atgaactgag
     4141 gggacaggat gtcccaggcg aagggcaggg ggccaccctt ggtcaccttc agcttggcgg
     4201 tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt
     4261 tcacggagcc ctccatgtgc accttgaagc gcatgaactc cttgatgatg gccatgttat
     4321 cctcctcgcc cttgctcacc atcgaagcct caacctttct cttcttcttt ggcgacatgg
     4381 tggcgaccgg cagatctgat cggtcccggt gtcttctatg gaggtcaaaa cagcgtggat
     4441 ggcgtctcca gggatctgac ggttcactaa accagctctg cttatatagg cctcccaccg
     4501 tacacgccta gacttgtctg gctcagtatg tctggctcct tctgtctggc tttcaatgtc
     4561 tggctacaaa tgtctggcta acgttgtctg gcttggactg tctggctcgt tatgtctggc
     4621 tgaatgtgtc tggctctgat tgtctggctg ctgatgtctg gcttaccctg tctggctgac
     4681 tacgcgtgtc gacggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     4741 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     4801 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     4861 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     4921 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     4981 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     5041 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     5101 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5161 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     5221 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5281 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     5341 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     5401 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     5461 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     5521 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     5581 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5641 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5701 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5761 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5821 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5881 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5941 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6001 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     6061 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6121 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6181 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6241 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6301 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6361 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6421 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     6481 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     6541 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     6601 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6661 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6721 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6781 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6841 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6901 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6961 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7021 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7081 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7141 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7201 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7261 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7321 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7381 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7441 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7501 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7561 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7621 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7681 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7741 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7801 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7861 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7921 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7981 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     8041 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     8101 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     8161 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     8221 aacaaaagct ggagctgcaa gcttaa
//
