LOCUS       sequence-384485-       11545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tetO-OS*KM (Dox-inducible reprogramming lentiviral vector expressing
            mouse Oct4 + super-Sox + Klf4 + cMyc).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11545)
  AUTHORS   MacCarthy CM, Wu G, Malik V, Menuchin-Lasowski Y, Velychko T, Keshet
            G, Fan R, Bedzhov I, Church GM, Jauch R, Cojocaru V, Scholer HR, 
            Velychko S
  TITLE     Highly cooperative chimeric super-SOX induces naive pluripotency 
            across species.
  JOURNAL   Cell Stem Cell. 2024 Jan 4;31(1):127-147.e9. doi: 
            10.1016/j.stem.2023.11.010. Epub 2023 Dec 22.
  PUBMED    38141611
REFERENCE   2  (bases 1 to 11545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2023.11.010"; journalName: "Cell Stem Cell"; date: 
            "2024-01-4- 4"; volume: "31"; issue: "1"; pages: "127-147.e9"
COMMENT     Sequence Label: 
            pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4-e2a-cMyc
FEATURES             Location/Qualifiers
     source          1..11545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2251..2269,2270..2292,2293..2311,2312..2334,2335..2353,
                     2354..2376,2377..2395,2396..2418,2419..2437,2438..2460,
                     2461..2479,2480..2502,2503..2521)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     2589..2613
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2679..2688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3756..3812
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5016..5069
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6540..6599
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     misc_feature    7987..8575
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8040..8060)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8458..8469)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8723..8903
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8950..8968)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9036..9140
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9141..9209,9210..10001)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9359..9378)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10172..10760
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10914..10931
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11171..11192
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11207..11212,11213..11230,11231..11237)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11245..11261
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11250..11272
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11269..11285
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11269..11285
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11335..11354
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca
     2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc atgtacggtg ggaggcctat
     2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccatggct ggacacctgg
     2701 cttcagactt cgccttctca cccccaccag gtgggggtga tgggtcagca gggctggagc
     2761 cgggctgggt ggatcctcga acctggctaa gcttccaagg gcctccaggt gggcctggaa
     2821 tcggaccagg ctcagaggta ttggggatct ccccatgtcc gcccgcatac gagttctgcg
     2881 gagggatggc atactgtgga cctcaggttg gactgggcct agtcccccaa gttggcgtgg
     2941 agactttgca gcctgagggc caggcaggag cacgagtgga aagcaactca gagggaacct
     3001 cctctgagcc ctgtgccgac cgccccaatg ccgtgaagtt ggagaaggtg gaaccaactc
     3061 ccgaggagtc ccaggacatg aaagccctgc agaaggagct agaacagttt gccaagctgc
     3121 tgaagcagaa gaggatcacc ttggggtaca cccaggccga cgtggggctc accctgggcg
     3181 ttctctttgg aaaggtgttc agccagacca ccatctgtcg cttcgaggcc ttgcagctca
     3241 gccttaagaa catgtgtaag ctgcggcccc tgctggagaa gtgggtggag gaagccgaca
     3301 acaatgagaa ccttcaggag atatgcaaat cggagaccct ggtgcaggcc cggaagagaa
     3361 agcgaactag cattgagaac cgtgtgaggt ggagtctgga gaccatgttt ctgaagtgcc
     3421 cgaagccctc cctacagcag atcactcaca tcgccaatca gcttgggcta gagaaggatg
     3481 tggttcgagt atggttctgt aaccggcgcc agaagggcaa aagatcaagt attgagtatt
     3541 cccaacgaga agagtatgag gctacaggga cacctttccc agggggggct gtatcctttc
     3601 ctctgccccc aggtccccac tttggcaccc caggctatgg aagcccccac ttcaccacac
     3661 tctactcagt cccttttcct gagggcgagg cctttccctc tgttcccgtc actgctctgg
     3721 gctctcccat gcattcaaac gctagcggca gcggcgccac gaacttctct ctgttaaagc
     3781 aagcaggaga tgttgaagaa aaccccgggc ctgcttgcat gtataacatg atggagacgg
     3841 agctgaagcc gccgggcccg cagcaagctt cggggggcgg cggcggagga ggcaacgcca
     3901 cggcggcggc gaccggcggc aaccagaaga acagcccgga ccgcgtcaag aggcccatga
     3961 acgccttcat ggtatggtcc cgggggcagc ggcgtaagat ggcccaggag aaccccaaga
     4021 tgcacaactc ggagatcagc aagcgcctgg gcgcggagtg gaaagcgttg accttggcag
     4081 agaagcggcc gttcatcgac gaggccaagc ggctgcgcgt gctgcatatg caggaccacc
     4141 ccaactacaa gtaccggccg cggcggcgca agcaggtgaa gcgcatgaag cgggtggagg
     4201 gaggcttcct gcacgctctc gtcgagcccc aggccggcgc gcttggtccc gagggcggcc
     4261 gcgtggccat ggatggcctg ggtctgcctt tcccggagcc gggctatccg gccggtcctc
     4321 cgctgatgtc tccgcacatg ggcccccact atcgggactg ccagggactg ggcgctcccg
     4381 cgctcgacgg ctaccctctg cccactccgg acacatcccc gctggatggc gtggagcagg
     4441 acccggcttt ctttgcagcc ccgctgccag gggactgccc ggcggccggc acctacactt
     4501 acgctccagt ctcggactat gcagtgtccg tagagccgcc cgctggcccc atgcgagtgg
     4561 ggccggaccc ctcgggccct gcgatgccgg ggatcctggc gccccccagc gctctgcacc
     4621 tgtactacgg cgcgatgggc tcgcccgccg caagtgcggg gcgcggtttc cacgcgcaac
     4681 cccagcagcc gctgcaaccg caggcaccgc ccccgccacc gcagcagcag cacccagcgc
     4741 acggccccgg gcaaccttcg ccccctcccg aggctctgcc ctgccgggat ggcacggaat
     4801 ccaaccagcc cactgagctc ctaggggagg tggaccgcac ggaatttgaa cagtatctgc
     4861 cctttgtgta taagcccgag atgggtcttc cctaccaggg acacgactgc ggagtgaacc
     4921 tctcagacag ccacggagcc atttcctccg tggtgtccga cgctagctca gcggtctact
     4981 attgcaacta ccccgacatt gcatgcggct ccggcgaggg caggggaagt cttctaacat
     5041 gcggggacgt ggaggaaaat cccggcccac tcgagatgag gcagccacct ggcgagtctg
     5101 acatggctgt cagcgacgct ctgctcccgt ccttctccac gttcgcgtcc ggcccggcgg
     5161 gaagggagaa gacactgcgt ccagcaggtg ccccgactaa ccgttggcgt gaggaactct
     5221 ctcacatgaa gcgacttccc ccacttcccg gccgccccta cgacctggcg gcgacggtgg
     5281 ccacagacct ggagagtggc ggagctggtg cagcttgcag cagtaacaac ccggccctcc
     5341 tagcccggag ggagaccgag gagttcaacg acctcctgga cctagacttt atcctttcca
     5401 actcgctaac ccaccaggaa tcggtggccg ccaccgtgac cacctcggcg tcagcttcat
     5461 cctcgtcttc cccagcgagc agcggccctg ccagcgcgcc ctccacctgc agcttcagct
     5521 atccgatccg ggccgggggt gacccgggcg tggctgccag caacacaggt ggagggctcc
     5581 tctacagccg agaatctgcg ccacctccca cggccccctt caacctggcg gacatcaatg
     5641 acgtgagccc ctcgggcggc ttcgtggctg agctcctgcg gccggagttg gacccagtat
     5701 acattccgcc acagcagcct cagccgccag gtggcgggct gatgggcaag tttgtgctga
     5761 aggcgtctct gaccacccct ggcagcgagt acagcagccc ttcggtcatc agtgttagca
     5821 aaggaagccc agacggcagc caccccgtgg tagtggcgcc ctacagcggt ggcccgccgc
     5881 gcatgtgccc caagattaag caagaggcgg tcccgtcctg cacggtcagc cggtccctag
     5941 aggcccattt gagcgctgga ccccagctca gcaacggcca ccggcccaac acacacgact
     6001 tccccctggg gcggcagctc cccaccagga ctacccctac actgagtccc gaggaactgc
     6061 tgaacagcag ggactgtcac cctggcctgc ctcttccccc aggattccat ccccatccgg
     6121 ggcccaacta ccctcctttc ctgccagacc agatgcagtc acaagtcccc tctctccatt
     6181 atcaagagct catgccaccg ggttcctgcc tgccagagga gcccaagcca aagaggggaa
     6241 gaaggtcgtg gccccggaaa agaacagcca cccacacttg tgactatgca ggctgtggca
     6301 aaacctatac caagagttct catctcaagg cacacctgcg aactcacaca ggcgagaaac
     6361 cttaccactg tgactgggac ggctgtgggt ggaaattcgc ccgctccgat gaactgacca
     6421 ggcactaccg caaacacaca gggcaccggc cctttcagtg ccagaagtgc gacagggcct
     6481 tttccaggtc ggaccacctt gccttacaca tgaagaggca ctttctcgag ggctcgggcc
     6541 agtgtactaa ttatgctctc ttgaaattgg ctggagatgt tgagagcaac ccaggtcccg
     6601 gcgcgccact ggatttcctt tgggcgttgg aaaccccgca gacagccacg acgatgcccc
     6661 tcaacgtgaa cttcaccaac aggaactatg acctcgacta cgactccgta cagccctatt
     6721 tcatctgcga cgaggaagag aatttctatc accagcaaca gcagagcgag ctgcagccgc
     6781 ccgcgcccag tgaggatatc tggaagaaat tcgagctgct tcccaccccg cccctgtccc
     6841 cgagccgccg ctccgggctc tgctctccat cctatgttgc ggtcgctacg tccttctccc
     6901 caagggaaga cgatgacggc ggcggtggca acttctccac cgccgatcag ctggagatga
     6961 tgaccgagtt acttggagga gacatggtga accagagctt catctgcgat cctgacgacg
     7021 agaccttcat caagaacatc atcatccagg actgtatgtg gagcggtttc tcagccgctg
     7081 ccaagctggt ctcggagaag ctggcctcct accaggctgc gcgcaaagac agcaccagcc
     7141 tgagccccgc ccgcgggcac agcgtctgct ccacctccag cctgtacctg caggacctca
     7201 ccgccgccgc gtccgagtgc attgacccct cagtggtctt tccctacccg ctcaacgaca
     7261 gcagctcgcc caaatcctgt acctcgtccg attccacggc cttctctcct tcctcggact
     7321 cgctgctgtc ctccgagtcc tccccacggg ccagccctga gcccctagtg ctgcatgagg
     7381 agacaccgcc caccaccagc agcgactctg aagaagagca agaagatgag gaagaaattg
     7441 atgtggtgtc tgtggagaag aggcaaaccc ctgccaagag gtcggagtcg ggctcatctc
     7501 catcccgagg ccacagcaaa cctccgcaca gcccactggt cctcaagagg tgccacgtct
     7561 ccactcacca gcacaactac gccgcacccc cctccacaag gaaggactat ccagctgcca
     7621 agagggccaa gttggacagt ggcagggtcc tgaagcagat cagcaacaac cgcaagtgct
     7681 ccagccccag gtcctcagac acggaggaaa acgacaagag gcggacacac aacgtcttgg
     7741 aacgtcagag gaggaacgag ctgaagcgca gcttttttgc cctgcgtgac cagatccctg
     7801 aattggaaaa caacgaaaag gcccccaagg tagtgatcct caaaaaagcc accgcctaca
     7861 tcctgtccat tcaagcagac gagcacaagc tcacctctga aaaggactta ttgaggaaac
     7921 gacgagaaca gttgaaacac aaactcgaac agcttcgaaa ctctggtgca taaatcgata
     7981 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     8041 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     8101 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     8161 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     8221 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     8281 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     8341 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     8401 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     8461 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     8521 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt
     8581 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     8641 ggggggactg gaagggctaa ttcactccca acgaagacaa gatcacctgc aggacaggcg
     8701 cgccctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     8761 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8821 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8881 gtcagtgtgg aaaatctcta gcacccgggc gattaaggaa agggctagat cattcttgaa
     8941 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     9001 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     9061 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     9121 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     9181 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     9241 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     9301 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     9361 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     9421 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     9481 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     9541 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     9601 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     9661 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     9721 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     9781 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     9841 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     9901 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     9961 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    10021 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    10081 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    10141 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    10201 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    10261 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
    10321 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    10381 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    10441 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    10501 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    10561 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    10621 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    10681 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    10741 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    10801 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    10861 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    10921 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    10981 cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg cagaggccga
    11041 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    11101 cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    11161 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    11221 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    11281 atgacatgat tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt
    11341 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
    11401 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
    11461 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    11521 aaatatgtac tacaaactta gtagt
//
