LOCUS       sequence-384494-        3961 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pTA106-Myacmini-KanR, donor plasmid with mini-transposon of McCAST 
            (Tn7575)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Hsieh SC, Peters JE
  TITLE     Discovery and characterization of novel type I-D CRISPR-guided 
            transposons identified among diverse Tn7-like elements in 
            cyanobacteria.
  JOURNAL   Nucleic Acids Res. 2023 Jan 25;51(2):765-782. doi: 
            10.1093/nar/gkac1216.
  PUBMED    36537206
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-01-25- 25"; volume: "51"; issue: 
            "2"; pages: "765-782"
COMMENT     Sequence Label: pOPO640
FEATURES             Location/Qualifiers
     source          1..3961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      320..542
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             590..1540
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     protein_bind    complement(2156..2203)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2590..3384
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2644..2663)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3254..3273
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(3492..3539)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 cccgccataa aacgccctga gaagcccgtg acgggctttt cttgtattat gggtagtttc
       61 cttgcatgaa tccataaaag gcgcctgtag tgccatttac ccccattcac tgccagagcc
      121 gtgagcgcag cgaactgaat gtcacgaaaa agacagcgac tcaggtgcct gatggtcgga
      181 gacaaaagga atattcagcg atttgcccga gcttgcgagg gtgctactta agcctttagg
      241 gttttaaggt ctgttttgta gaggagcaaa cagcgtttgc gacatccttt tgtaatactg
      301 cggaactgac taaagtagtg agttatacac agggctggga tctattcttt ttatcttttt
      361 ttattctttc tttattctat aaattataac cacttgaata taaacaaaaa aaacacacaa
      421 aggtctagcg gaatttacag agggtctagc agaatttaca agttttccag caaaggtcta
      481 gcagaattta cagataccca caactcaaag gaaaaggact agtaattatc attgactagc
      541 ccatctcaat tggtatagtg attaaaatca cctagaccaa ttgagatgta tgtctgaatt
      601 agttgttttc aaagcaaatg aactagcgat tagtcgctat gacttaacgg agcatgaaac
      661 caagctaatt ttatgctgtg tggcactact caaccccacg attgaaaacc ctacaaggaa
      721 agaacggacg gtatcgttca cttataacca atacgctcag atgatgaaca tcagtaggga
      781 aaatgcttat ggtgtattag ctaaagcaac cagagagctg atgacgagaa ctgtggaaat
      841 caggaatcct ttggttaaag gctttgagat tttccagtgg acaaactatg ccaagttctc
      901 aagcgaaaaa ttagaattag tttttagtga agagatattg ccttatcttt tccagttaaa
      961 aaaattcata aaatataatc tggaacatgt taagtctttt gaaaacaaat actctatgag
     1021 gatttatgag tggttattaa aagaactaac acaaaagaaa actcacaagg caaatataga
     1081 gattagcctt gatgaattta agttcatgtt aatgcttgaa aataactacc atgagtttaa
     1141 aaggcttaac caatgggttt tgaaaccaat aagtaaagat ttaaacactt acagcaatat
     1201 gaaattggtg gttgataagc gaggccgccc gactgatacg ttgattttcc aagttgaact
     1261 agatagacaa atggatctcg taaccgaact tgagaacaac cagataaaaa tgaatggtga
     1321 caaaatacca acaaccatta catcagattc ctacctacat aacggactaa gaaaaacact
     1381 acacgatgct ttaactgcaa aaattcagct caccagtttt gaggcaaaat ttttgagtga
     1441 catgcaaagt aagtatgatc tcaatggttc gttctcatgg ctcacgcaaa aacaacgaac
     1501 cacactagag aacatactgg ctaaatacgg aaggatctga ggttcttatg gctcttgtat
     1561 ctatcagtga agcatcaaga ctaacaaaca aaagtagaac aactgttcac cgttacatat
     1621 caaagggaaa actgtccata tgcacagatg aaaacggtgt aaaaaagata gatacatcag
     1681 agcttttacg agtttttggt gcattcaaag ctgttcacca tgaacagatc gacaatgtaa
     1741 cagatgaaca gcatgtaaca cctaatagaa caggtgaaac cagtaaaaca aagcaactag
     1801 aacatgaaat tgaacacctg agacaacttg ttacagctca acagtcacac atagacagcc
     1861 tgaaacaggc gatgctgctt atcgaatcaa agctgccgac aacacgggag ccagtgacgc
     1921 ctcccgtggg gaaaaaatca tggcaattct ggaagaaata gcgctgaccc ctaggttgga
     1981 ttaggcaagc tacgacttta accataagtt ggacatccgt tcatcttatg cttcaaaagt
     2041 cgtctgttca tcttatgctt caaaaagaac ttaaaggttc ttaactaagt tttttgaaga
     2101 cacttttaca tgaggagctg aatgcatgat ctcgaattag cttcaaaagc gctctgaagt
     2161 tcctatactt tctagagaat aggaacttcg gaataggaac ttcaagatcc cctgattccc
     2221 tttgtcaaca gcaatggata attcgattta acaaatgcat ggcgcaaggg ctgctaaagg
     2281 aagcggaaca cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc
     2341 tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt
     2401 gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg
     2461 ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc
     2521 ttgccgccaa ggatctgatg gcgcagggga tcaagatctg atcaagagac aggatgagga
     2581 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     2641 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     2701 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     2761 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     2821 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     2881 ccggggcagg atctcctgtc atcccacctt gctcctgccg agaaagtatc catcatggct
     2941 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     3001 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     3061 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     3121 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     3181 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     3241 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     3301 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     3361 cgccttcttg acgagttctt ctgaattgaa aaaggaagag tatgaggatc caacatttcc
     3421 aatcactagt gaattatcta gaattattcc attgagtaag tttttaagca catcagcttc
     3481 aaaagcgctc tgaagttcct atactttcta gagaatagga acttcggaat aggtacttca
     3541 agatccccaa ttcgagatcc aattaccagc accatgatct ttataattct taatggtgaa
     3601 gaataagaat cgaccaatca taaagttttg tttacaaaat tttgttcagt ttatgcctct
     3661 atctttgtac tgtcgttcaa cttatgatta aaaacttgga agacctaagg ttaagacagc
     3721 atggtgggat cagctttttc tcccatcttt gctcaactta tggttcaaac aatgttcaac
     3781 ttatggttaa agtcgtacaa gcctaagctg tcctcgaggg ctgttgatga taccgctgcc
     3841 ttactgggtg cattagccag tctgaatgac ctgtcacggg ataatccgaa gtggtcagac
     3901 tggaaaataa gagggcagga actgctgaac agcaaaaagt cagatagcac cacatagcag
     3961 a
//
