LOCUS       sequence-384495-        8261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirial plasmid for nuclear iRFP-720 expression with puromycin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Gabriel CH, Del Olmo M, Rizki Widini A, Roshanbin R, Woyde J, Hamza 
            E, Gutu NN, Zehtabian A, Ewers H, Granada A, Herzel H, Kramer A
  TITLE     Circadian period is compensated for repressor protein turnover rates
            in single cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Aug 20;121(34):e2404738121. doi: 
            10.1073/pnas.2404738121. Epub 2024 Aug 14.
  PUBMED    39141353
REFERENCE   2  (bases 1 to 8261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-08-20"; volume: "121(34):e2404738121. 
            doi"; pages: " 10.1073/pnas.2404738121. Epub 2024 Aug 14"
COMMENT     Sequence Label: pL6-H2B-iRFP720-puro
FEATURES             Location/Qualifiers
     source          1..8261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     31..51
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        33..51
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        79..305
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             306..486
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    533..658
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1151..1384
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1569..1613
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1896..2406
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2325..2343
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2418..2795
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             2814..3761
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 720 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP720
                     /note="derived from Rhodopseudomonas palustris RpBphP2"
                     /translation="AEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGSD
                     NLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFIG
                     SWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKITG
                     FDRVMIYRFASDFSGSVIAEDRCAEVESKLGLHYPASFIPAQARRLYTINPVRIIPDIN
                     YRPVPVTPDLNPVTGRPIDLSFAILRSVSPNHLEFMRNIGMHGTMSISILRGERLWGLI
                     VCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     primer_bind     complement(3801..3821)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3806..4135
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3986..4121
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4184..4786
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     4683..4703
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4858..5091
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5163..5284
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5200..5219)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5254..5273
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5324..5459
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5479..5498)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5480..5498)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5508..5525)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5508..5524)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5517..5539)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5666..6121
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5753..5772)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5963..5984
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6147..6251
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6252..6320,6321..7112)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6470..6489)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7283..7871
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7772..7791
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8025..8042
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8159..8180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8195..8200,8201..8218,8219..8225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8233..8249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8238..8260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(8257..8261,1..12)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(8257..8261,1..12)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
       61 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
      121 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
      181 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
      241 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
      301 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
      361 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
      421 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
      481 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
      541 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
      601 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
      661 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
      721 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
      781 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
      841 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
      901 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
      961 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     1021 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     1081 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     1141 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     1201 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     1261 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     1321 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     1381 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     1441 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     1501 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     1561 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     1621 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     1681 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     1741 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     1801 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     1861 ctcgacggta tcgatcactt tggccgcggc tcgagggggt tggggttgcg ccttttccaa
     1921 ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc
     1981 ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc
     2041 gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt
     2101 tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc
     2161 ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc
     2221 aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag
     2281 gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc
     2341 aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct
     2401 ccccaggggg atctaccatg ccagagccag cgaagtctgc tcccgccccg aaaaagggct
     2461 ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca
     2521 aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct gacaccggca
     2581 tttcgtccaa ggccatgggc atcatgaact cgtttgtgaa cgacattttc gagcgcatcg
     2641 caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc tccagggaga
     2701 tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc gtgtccgagg
     2761 gtactaaggc catcaccaag tacaccagcg ctaaggatcc accggtcgcc accgcggaag
     2821 gatccgtcgc caggcagcct gacctcttga cctgcgacga tgagccgatc catatccccg
     2881 gtgccatcca accgcatgga ctgctgctcg ccctcgccgc cgacatgacg atcgttgccg
     2941 gcagcgacaa ccttcccgaa ctcaccggac tggcgatcgg cgccctgatc ggccgctctg
     3001 cggccgatgt cttcgactcg gagacgcaca accgtctgac gatcgccttg gccgagcccg
     3061 gggcggccgt cggagcaccg atcactgtcg gcttcacgat gcgaaaggac gcaggcttca
     3121 tcggctcctg gcatcgccat gatcagctca tcttcctcga gctcgagcct ccccagcggg
     3181 acgtcgccga gccgcaggcg ttcttccgcc gcaccaacag cgccatccgc cgcctgcagg
     3241 ccgccgaaac cttggaaagc gcctgcgccg ccgcggcgca agaggtgcgg aagattaccg
     3301 gcttcgatcg ggtgatgatc tatcgcttcg cctccgactt cagcgggtcc gtgatcgcag
     3361 aggatcggtg cgccgaggtc gagtcaaaac taggcctgca ctatcctgcc tcattcatcc
     3421 cggcgcaggc ccgtcggctc tataccatca acccggtacg gatcattccc gatatcaatt
     3481 atcggccggt gccggtcacc ccagacctca atccggtcac cgggcggccg attgatctta
     3541 gcttcgccat cctgcgcagc gtctcgccca accatctgga gttcatgcgc aacataggca
     3601 tgcacggcac gatgtcgatc tcgattttgc gcggcgagcg actgtgggga ttgatcgttt
     3661 gccatcaccg aacgccgtac tacgtcgatc tcgatggccg ccaagcctgc gagctagtcg
     3721 cccaggttct ggcctggcag atcggcgtga tggaagagta aacgcgtacc ggttagtaat
     3781 gagtttggaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     3841 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     3901 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3961 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4021 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     4081 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     4141 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgatggcca ccgagtacaa
     4201 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc
     4261 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg
     4321 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     4381 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     4441 ggcggtgttc gccgagatcg gctcgcgcat ggccgagttg agcggttccc ggctggccgc
     4501 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     4561 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     4621 ccccggagtg gaggcggccg agcgcgctgg ggtgcccgcc ttcctggaga cctccgcgcc
     4681 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     4741 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaggta cctttaagac
     4801 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     4861 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     4921 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4981 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     5041 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     5101 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     5161 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5221 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5281 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     5341 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     5401 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5461 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     5521 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     5581 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     5641 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5701 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5761 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5821 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5881 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5941 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6001 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6061 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     6121 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6181 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6241 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6301 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6361 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6421 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6481 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6541 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6601 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6661 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6721 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6781 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6841 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6901 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6961 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7021 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7081 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7141 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7201 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7261 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7321 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7381 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     7441 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7501 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7561 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7621 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7681 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7741 gaagaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7801 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7861 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7921 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7981 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8041 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     8101 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     8161 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     8221 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg a
//
