LOCUS       sequence-384496-       13013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Cas9/sgRNA vector targeting C-terminus of hCRY2, works 
            with Addgene 189983-189986.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13013)
  AUTHORS   Gabriel CH, Del Olmo M, Rizki Widini A, Roshanbin R, Woyde J, Hamza 
            E, Gutu NN, Zehtabian A, Ewers H, Granada A, Herzel H, Kramer A
  TITLE     Circadian period is compensated for repressor protein turnover rates
            in single cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Aug 20;121(34):e2404738121. doi: 
            10.1073/pnas.2404738121. Epub 2024 Aug 14.
  PUBMED    39141353
REFERENCE   2  (bases 1 to 13013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-08-20"; volume: "121(34):e2404738121. 
            doi"; pages: " 10.1073/pnas.2404738121. Epub 2024 Aug 14"
COMMENT     Sequence Label: pLentiCriprV2-sgRNA-CRY2-#1
FEATURES             Location/Qualifiers
     source          1..13013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(14..33)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     260..280
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(289..717)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(399..420)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     611..630
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    763..987
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     976..993
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             1019..1252
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    1324..1912
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1430..1441
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1839..1859
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1928..2524)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2011..2031)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             complement(2525..2581)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2591..2614)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2615..2662)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(2663..6766)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(2663..6766)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     regulatory      6763..6772
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6791..7002)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_RNA        complement(7064..7139)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(7161..7409)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(7219..7238)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(7389..7409)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    7460..7577
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7875..7919)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8104..8337
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8830..8955
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9002..9182
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(9200..9398)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9225..9245)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9400..9779
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     9951..9970
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10045..10149
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10150..10218,10219..11010)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10368..10387)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11181..11769
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11670..11689
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11923..11940
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12057..12078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12093..12098,12099..12116,12117..12123)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12131..12147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12136..12158
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12155..12171
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12155..12171
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    12220..12341
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12231..12250)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     12285..12304
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(12471..12845)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(12864..12911)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(join(12959..13013,1..275))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(12973..13013,1..95)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
       61 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
      121 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
      181 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
      241 gagcctgggg actttccaca ccctaactga cacacattcc acagaattaa ttcgcgttaa
      301 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
      361 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
      421 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
      481 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
      541 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
      601 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
      661 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtggg
      721 gataccccct agagccccag ctggttcttt ccgcctcaga agccatagag cccaccgcat
      781 ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac
      841 cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg
      901 aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa
      961 cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgggtttaa acgggccctg
     1021 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
     1081 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
     1141 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
     1201 gtacaagcaa aaagcagatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc
     1261 ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaagtc
     1321 gacgcgggga ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg
     1381 gaagaggccg cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg
     1441 aagggacgta gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc
     1501 aaggaaagga cgatgatttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag
     1561 cccctgtcca gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg
     1621 ggaaagcgaa agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg
     1681 ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct
     1741 cataaagaga cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag
     1801 caatagcatg atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat
     1861 agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga ttgtcgactt
     1921 aacgcgttca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct
     1981 cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg
     2041 aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga
     2101 cggcgctgcc cagacccttg ccctggtggt cgggcgagac tccgacggtg gccaggaacc
     2161 acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca
     2221 gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt
     2281 cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct
     2341 tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga
     2401 tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga
     2461 gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact
     2521 cggtcggtcc aggattctct tcgacatctc cggcttgttt cagcagagag aagtttgttg
     2581 cgccggatcc cttatcgtca tcgtctttgt aatctttctt cttcttagcc tgtccagcct
     2641 tctttgtggc ggcaggtcgc ttgtcgcctc ccagctgaga caggtcgatc cgtgtctcgt
     2701 acaggccggt gatgctctgg tggatcaggg tggcgtccag cacctctttg gtgctggtgt
     2761 acctcttccg gtcgatggtg gtgtcaaagt acttgaaggc ggcaggggct cccagattgg
     2821 tcagggtaaa caggtggatg atattctcgg cctgctctct gatgggctta tcccggtgct
     2881 tgttgtaggc ggacagcact ttgtccagat tagcgtcggc caggatcact ctcttggaga
     2941 actcgctgat ctgctcgatg atctcgtcca ggtagtgctt gtgctgttcc acaaacagct
     3001 gtttctgctc attatcctcg ggggagccct tcagcttctc atagtggctg gccaggtaca
     3061 ggaagttcac atatttggag ggcagggcca gttcgtttcc cttctgcagt tcgccggcag
     3121 aggccagcat tctcttccgg ccgttttcca gctcgaacag ggagtactta ggcagcttga
     3181 tgatcaggtc ctttttcact tctttgtagc ccttggcttc cagaaagtcg atgggattct
     3241 tctcgaagct gcttctttcc atgatggtga tccccagcag ctctttcaca ctcttcagtt
     3301 tcttggactt gcccttttcc actttggcca ccaccagcac agaataggcc acggtggggc
     3361 tgtcgaagcc gccgtacttc ttagggtccc agtccttctt tctggcgatc agcttatcgc
     3421 tgttcctctt gggcaggata gactctttgc tgaagccgcc tgtctgcacc tcggtctttt
     3481 tcacgatatt cacttggggc atgctcagca ctttccgcac ggtggcaaaa tcccggccct
     3541 tatcccacac gatctccccg gtttcgccgt ttgtctcgat cagaggccgc ttccggatct
     3601 cgccgttggc cagggtaatc tcggtcttga aaaagttcat gatgttgctg tagaagaagt
     3661 acttggcggt agccttgccg atttcctgct cgctcttggc gatcatcttc cgcacgtcgt
     3721 acaccttgta gtcgccgtac acgaactcgc tttccagctt agggtacttt ttgatcaggg
     3781 cggttcccac gacggcgttc aggtaggcgt cgtgggcgtg gtggtagttg ttgatctcgc
     3841 gcactttgta aaactggaaa tccttccgga aatcggacac cagcttggac ttcagggtga
     3901 tcactttcac ttcccggatc agcttgtcat tctcgtcgta cttagtgttc atccgggagt
     3961 ccaggatctg tgccacgtgc tttgtgatct gccgggtttc caccagctgt ctcttgatga
     4021 agccggcctt atccagttcg ctcaggccgc ctctctcggc cttggtcaga ttgtcgaact
     4081 ttctctgggt aatcagcttg gcgttcagca gctgccgcca gtagttcttc atcttcttca
     4141 cgacctcttc ggagggcacg ttgtcgctct tgccccggtt cttgtcgctt ctggtcagca
     4201 ccttgttgtc gatggagtcg tccttcagaa agctctgagg cacgatatgg tccacatcgt
     4261 agtcggacag ccggttgatg tccagttcct ggtccacgta catatcccgc ccattctgca
     4321 ggtagtacag gtacagcttc tcgttctgca gctgggtgtt ttccacgggg tgttctttca
     4381 ggatctggct gcccagctct ttgatgccct cttcgatccg cttcattctc tcgcggctgt
     4441 tcttctgtcc cttctgggtg gtctggttct ctctggccat ttcgatcacg atgttctcgg
     4501 gcttgtgccg gcccatcact ttcacgagct cgtccaccac cttcactgtc tgcaggatgc
     4561 ccttcttaat ggcggggctg ccggccagat tggcaatgtg ctcgtgcagg ctatcgccct
     4621 ggccggacac ctgggctttc tggatgtcct ctttaaaggt caggctgtcg tcgtggatca
     4681 gctgcatgaa gtttctgttg gcgaagccgt cggacttcag gaaatccagg attgtcttgc
     4741 cggactgctt gtcccggatg ccgttgatca gcttccggct cagcctgccc cagccggtgt
     4801 atctccgccg cttcagctgc ttcatcactt tgtcgtcgaa caggtgggca taggttttca
     4861 gccgttcctc gatcatctct ctgtcctcaa acagtgtcag ggtcagcacg atatcttcca
     4921 gaatgtcctc gttttcctca ttgtccagga agtccttgtc cttgataatt ttcagcagat
     4981 cgtggtatgt gcccagggag gcgttgaacc gatcttccac gccggagatt tccacggagt
     5041 cgaagcactc gattttcttg aagtagtcct ctttcagctg cttcacggtc actttccggt
     5101 tggtcttgaa cagcaggtcc acgatggcct ttttctgctc gccgctcagg aaggcgggct
     5161 ttctcattcc ctcggtcacg tatttcactt tggtcagctc gttatacacg gtgaagtact
     5221 cgtacagcag gctgtgcttg ggcagcacct tctcgttggg caggttctta tcgaagttgg
     5281 tcatccgctc gatgaagctc tgggcggaag cgcccttgtc caccacttcc tcgaagttcc
     5341 agggggtgat ggtttcctcg ctctttctgg tcatccaggc gaatctgctg tttcccctgg
     5401 ccagagggcc cacgtagtag gggatgcgga aggtcaggat cttctcgatc ttttcccggt
     5461 tgtccttcag gaatgggtaa aaatcttcct gccgccgcag aatggcgtgc agctctccca
     5521 ggtggatctg gtgggggatg ctgccgttgt cgaaggtccg ctgcttccgc agcaggtcct
     5581 ctctgttcag cttcacgagc agttcctcgg tgccgtccat cttttccagg atgggcttga
     5641 tgaacttgta gaactcttcc tggctggctc cgccgtcaat gtagccggcg tagccgttct
     5701 tgctctggtc gaagaaaatc tctttgtact tctcaggcag ctgctgccgc acgagagctt
     5761 tcagcagggt caggtcctgg tggtgctcgt cgtatctctt gatcatagag gcgctcaggg
     5821 gggccttggt gatctcggtg ttcactctca ggatgtcgct cagcaggatg gcgtcggaca
     5881 ggttcttggc ggccagaaac aggtcggcgt actggtcgcc gatctgggcc agcaggttgt
     5941 ccaggtcgtc gtcgtaggtg tccttgctca gctgcagttt ggcatcctcg gccaggtcga
     6001 agttgctctt gaagttgggg gtcaggccca ggctcagggc aatcaggttt ccgaacaggc
     6061 cattcttctt ctcgccgggc agctgggcga tcagattttc cagccgtctg ctcttgctca
     6121 gtctggcaga caggatggcc ttggcgtcca cgccgctggc gttgatgggg ttttcctcga
     6181 acagctggtt gtaggtctgc accagctgga tgaacagctt gtccacgtcg ctgttgtcgg
     6241 ggttcaggtc gccctcgatc aggaagtggc cccggaactt gatcatgtgg gccagggcca
     6301 gatagatcag ccgcaggtcg gccttgtcgg tgctgtccac cagtttcttt ctcaggtggt
     6361 agatggtggg gtacttctcg tggtaggcca cctcgtccac gatgttgccg aagatggggt
     6421 gccgctcgtg cttcttatcc tcttccacca ggaaggactc ttccagtctg tggaagaagc
     6481 tgtcgtccac cttggccatc tcgttgctga agatctcttg cagatagcag atccggttct
     6541 tccgtctggt gtatcttctt ctggcggttc tcttcagccg ggtggcctcg gctgtttcgc
     6601 cgctgtcgaa cagcagggct ccgatcaggt tcttcttgat gctgtgccgg tcggtgttgc
     6661 ccagcacctt gaatttcttg ctgggcacct tgtactcgtc ggtgatcacg gcccagccca
     6721 cagagttggt gccgatgtcc aggccgatgc tgtacttctt gtccatggtg gcagcgctct
     6781 agaaccggtc ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta
     6841 ctgcacttat atacggttct cccccaccct cgggaaaaag gcggagccag tacacgacat
     6901 cactttccca gtttaccccg cgccaccttc tctaggcacc ggatcaattg ccgacccctc
     6961 cccccaactt ctcggggact gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc
     7021 caattcccac tcctttcaag acctagctag cgaattcaaa aaagcaccga ctcggtgcca
     7081 ctttttcaag ttgataacgg actagcctta ttttaacttg ctatttctag ctctaaaacg
     7141 aggttgaatc taggctgctc ggtgtttcgt cctttccaca agatatataa agccaagaaa
     7201 tcgaaatact ttcaagttac ggtaagcata tgatagtcca ttttaaaaca taattttaaa
     7261 actgcaaact acccaagaaa ttattacttt ctacgtcacg tattttgtac taatatcttt
     7321 gtgtttacag tcaaattaat tctaattatc tctctaacag ccttgtatcg tatatgcaaa
     7381 tatgaaggaa tcatgggaaa taggccctcg gtaccttaat taaccaaact ggatctctgc
     7441 tgtccctgta ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag
     7501 tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc
     7561 ccccttttct tttaaaattg tggatgaata ctgccatttg tctgcagaat tggcgcacgc
     7621 agtgccgatc cgttcactaa tcgaatggat ctgtctctgt ctctctctcc accttcttct
     7681 tctattcctt cgggcctgtc gggtcccctc ggggttggga ggtgggtctg aaacgataat
     7741 ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac tattcttaaa
     7801 cctaccaagc ctcctactat cattatgaat aattttatat accacagcca atttgttatg
     7861 ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg ttcattcttt
     7921 tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta attgttaatt
     7981 tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga tttattactc
     8041 caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca accccaaatc
     8101 cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc ctggagctgc
     8161 ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa
     8221 ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt cagcgtcatt
     8281 gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa agctcctatt
     8341 cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg tgctactcct
     8401 aatggttcaa tttttactac tttatattta tataattcac ttctccaatt gtccctcata
     8461 tctcctcctc caggtctgaa gatcagcggc cgcttgctgt gcggtggtct tacttttgtt
     8521 ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct tttatctcta tcctttgatg
     8581 cacacaatag agggttgcta ctgtattata taatgatcta agttcttctg atcctgtctg
     8641 aagggatggt tgtagctgtc ccagtatttg tctacagcct tctgatgttt ctaacaggcc
     8701 aggattaact gcgaatcgtt ctagctccct gcttgcccat actatatgtt ttaatttata
     8761 ttttttcttt ccccctggcc ttaaccgaat tttttcccat cgcgatctaa ttctcccccg
     8821 cttaatactg acgctctcgc acccatctct ctccttctag cctccgctag tcaaaatttt
     8881 tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt
     8941 cctgcgtcga gagagctcct ctggtttccc tttcgctttc aagtccctgt tcgggcgcca
     9001 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca
     9061 cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc
     9121 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
     9181 ccagtacagg caaaacgcgc tgcttatata gacctcccac cgtacacgcc taccgcccat
     9241 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
     9301 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
     9361 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
     9421 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
     9481 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
     9541 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
     9601 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
     9661 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
     9721 gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtca
     9781 acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt
     9841 cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc tcgcgcacta
     9901 ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg cggcatcaga
     9961 gcagattgta ctgagagtgc accatagggg atcgggagat ctcccgatcc gtcgacgtca
    10021 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
    10081 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
    10141 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
    10201 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
    10261 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
    10321 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
    10381 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
    10441 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
    10501 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
    10561 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
    10621 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
    10681 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
    10741 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
    10801 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
    10861 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
    10921 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
    10981 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
    11041 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
    11101 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    11161 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    11221 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    11281 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
    11341 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    11401 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    11461 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    11521 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    11581 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    11641 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    11701 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    11761 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    11821 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    11881 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    11941 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
    12001 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
    12061 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
    12121 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
    12181 gccaagctct agctagaggt cgacggtata cagacatgat aagatacatt gatgagtttg
    12241 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
    12301 ttgctttatt tgtaaccatt ataagctgca ataaacaagt tggggtgggc gaagaactcc
    12361 agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag
    12421 cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc
    12481 tcctcggcca cgaagtgcac gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc
    12541 cacggctgct cgccgatctc ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac
    12601 acgacctccg accactcggc gtacagctcg tccaggccgc gcacccacac ccaggccagg
    12661 gtgttgtccg gcaccacctg gtcctggacc gcgctgatga acagggtcac gtcgtcccgg
    12721 accacaccgg cgaagtcgtc ctccacgaag tcccgggaga acccgagccg gtcggtccag
    12781 aactcgaccg ctccggcgac gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg
    12841 gccatggttt agttcctcac cttgtcgtat tatactatgc cgatatacta tgccgatgat
    12901 taattgtcaa cacgtgctga tcagatccga aaatggatat acaagctccc gggagctttt
    12961 tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc tca
//
