LOCUS       sequence-384508-        9727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector to knock in firefly Luciferase C-terminal to human CRY1
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9727)
  AUTHORS   Gabriel CH, Del Olmo M, Zehtabian A, Jager M, Reischl S, van Dijk H,
            Ulbricht C, Rakhymzhan A, Korte T, Koller B, Grudziecki A, Maier B, 
            Herrmann A, Niesner R, Zemojtel T, Ewers H, Granada AE, Herzel H, 
            Kramer A
  TITLE     Live-cell imaging of circadian clock protein dynamics in 
            CRISPR-generated knock-in cells.
  JOURNAL   Nat Commun. 2021 Jun 18;12(1):3796. doi: 10.1038/s41467-021-24086-9.
  PUBMED    34145278
REFERENCE   2  (bases 1 to 9727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-18"; volume: "12(1):3796. doi"; pages: " 
            10.1038/s41467-021-24086-9"
COMMENT     Sequence Label: pDB-hCRY1-Luciferase-hvTK-bla
FEATURES             Location/Qualifiers
     source          1..9727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..48
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             55..1701
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(118..136)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             1708..1725
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1735..1758
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1787..1820
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(1828..1861)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(1944..1964)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1949..2279
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2130..2265
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2192..2211
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      2329..2338
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2335..2337,2338..2340,2341..3051)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2380..2401)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2641..2660)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             3067..3123
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3130..3525
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    3537..3658
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3574..3593)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3628..3647
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    3686..3719
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(3720..3753)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4714..4917
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4868..4888
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4914..4938
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5013..6143
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(6447..6463)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6447..6463)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6460..6482)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6471..6487
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6495..6501,6502..6519,6520..6525))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6540..6561
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6678..6695)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6849..7437)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6929..6948)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7608..8399,8400..8468))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8231..8250
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8469..8573)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8641..8659
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     8819..8838
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcac cggtgaagac
       61 gccaaaaaca taaagaaagg cccggcgcca ttctatcctc tagaggatgg aaccgctgga
      121 gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca
      181 gatgcacata tcgaggtgaa catcacgtac gcggaatact tcgaaatgtc cgttcggttg
      241 gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa
      301 aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg
      361 cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgaacat ttcgcagcct
      421 accgtagtgt ttgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaaatta
      481 ccaataatcc agaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg
      541 atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtacca
      601 gagtcctttg atcgtgacaa aacaattgca ctgataatga attcctctgg atctactggg
      661 ttacctaagg gtgtggccct tccgcataga actgcctgcg tcagattctc gcatgccaga
      721 gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc
      781 catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc
      841 ttaatgtata gatttgaaga agagctgttt ttacgatccc ttcaggatta caaaattcaa
      901 agtgcgttgc tagtaccaac cctattttca ttcttcgcca aaagcactct gattgacaaa
      961 tacgatttat ctaatttaca cgaaattgct tctgggggcg cacctctttc gaaagaagtc
     1021 ggggaagcgg ttgcaaaacg cttccatctt ccagggatac gacaaggata tgggctcact
     1081 gagactacat cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt
     1141 aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc
     1201 gttaatcaga gaggcgaatt atgtgtcaga ggacctatga ttatgtccgg ttatgtaaac
     1261 aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata
     1321 gcttactggg acgaagacga acacttcttc atagttgacc gcttgaagtc tttaattaaa
     1381 tacaaaggat atcaggtggc ccccgctgaa ttggaatcga tattgttaca acaccccaac
     1441 atcttcgacg cgggcgtggc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc
     1501 gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc
     1561 agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg
     1621 aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag
     1681 aagggcggaa agtccaaatt gactagtcac catcaccatc accatggtag cggtgactac
     1741 aaagacgatg acgacaaggc tagcaaagat ctgaagttcc tattccgaag ttcctattct
     1801 tcaaatagta taggaacttc agatctaata acttcgtata gcatacatta tacgaagtta
     1861 ttttgaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgttgagctg
     1921 atttaacaaa aatttaacgc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     1981 ctccccaggc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     2041 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     2101 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     2161 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     2221 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     2281 agctattgta tatccatttt cggatctgat cagcactgag caggtaccgc caccatggtg
     2341 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     2401 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     2461 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     2521 accaccctga cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     2581 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     2641 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     2701 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     2761 gagtacaacg ccatcagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc
     2821 aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     2881 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     2941 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     3001 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggaattcgga
     3061 agcggagcta ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga
     3121 cctcacgtgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct
     3181 acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc
     3241 gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa
     3301 ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg
     3361 atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc
     3421 gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt
     3481 gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaactcga gaccccaact
     3541 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     3601 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     3661 atgtctgtat agaagttcct attccgaagt tcctattctt caaatagtat aggaacttca
     3721 taacttcgta tagcatacat tatacgaagt tatttgatta attaagcgat cgcttcgaat
     3781 gcactaatta ggtaaatatt ttagagctgt atttcttgct ttagaagagt atataattaa
     3841 cataaattaa gataatttca aaaatggagc aaatctctat tttcaaacca gaaaatcttg
     3901 aggcattaat ttttaagcaa tttttacaaa ctcagttaat ttttggtcaa gagacatgca
     3961 tctgtactgg agaaattgtt gcaccagttt tatattcatc tgaaccaatg ctctttaaat
     4021 tagagatgtt tatgattttg tggtcaagtt ttttcttaga aaaagacaac ttttttattt
     4081 ccttactatg taactatgag tctaaaacaa ttaaagtggc ctgtttattt tagtgacatt
     4141 aatataatct ttttatgaac tttcccctaa atctttgcct cttaaatgtt gataaatttc
     4201 ttttatctgt tatgatgctt ctaaaatcta aattatttcc aatttgggaa tagttcaaaa
     4261 ttttttaaaa tgctggccct tattagaagt atcagaaagc cttgcctgca ttcaatttaa
     4321 ttggatttgg gatgtcattt tgtgatttaa attaatatga aaaatattta tacgttggat
     4381 ttgccagttt ttaaaaattt tctgtttctt cagtttctaa cactgtttac attttttatt
     4441 gcttagtttt tttatgtcaa cctaattaga ctataagtat cttgaagata aggtcaataa
     4501 acactcatca catttttgtc attgaattat ttgcaatcaa gctttaccta gttttttttt
     4561 cccccttaaa tcacagaaaa cattcaggag gaatactggt cgacaagctt gcatgcctgc
     4621 aggtcaattc cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     4681 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     4741 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     4801 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     4861 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     4921 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     4981 caccgggacc gatccagcct ggggatcccg acatggcttc gtacccctgc catcaacacg
     5041 cgtctgcgtt cgaccaggct gcgcgttctc gcggccatag caaccgacgt acggcgttgc
     5101 gccctcgccg gcagcaagaa gccacggaag tccgcctgga gcagaaaatg cccacgctac
     5161 tgcgggttta tatagacggt cctcacggga tggggaaaac caccaccacg caactgctgg
     5221 tggccctggg ttcgcgcgac gatatcgtct acgtacccga gccgatgact tactggcagg
     5281 tgctgggggc ttccgagaca atcgcgaaca tctacaccac acaacaccgc ctcgaccagg
     5341 gtgagatatc ggccggggac gcggcggtgg taatgacaag cgcccagata acaatgggca
     5401 tgccttatgc cgtgaccgac gccgttctgg ctcctcatat cgggggggag gctgggagct
     5461 cacatgcccc gcccccggcc ctcaccctca tcttcgaccg ccatcccatc gccgccctcc
     5521 tgtgctaccc ggccgcgcga taccttatgg gcagcatgac cccccaggcc gtgctggcgt
     5581 tcgtggccct catcccgccg accttgcccg gcacaaacat cgtgttgggg gcccttccgg
     5641 aggacagaca catcgaccgc ctggccaaac gccagcgccc cggcgagcgg cttgacctgg
     5701 ctatgctggc cgcgattcgc cgcgtttacg ggctgcttgc caatacggtg cggtatctgc
     5761 agggcggcgg gtcgtggcgg gaggattggg gacagctttc ggggacggcc gtgccgcccc
     5821 agggtgccga gccccagagc aacgcgggcc cacgacccca tatcggggac acgttattta
     5881 ccctgtttcg ggcccccgag ttgctggccc ccaacggcga cctgtacaac gtgtttgcct
     5941 gggccttgga cgtcttggcc aaacgcctcc gtcccatgca cgtctttatc ctggattacg
     6001 accaatcgcc cgccggctgc cgggacgccc tgctgcaact tacctccggg atggtccaga
     6061 cccacgtcac cacccccggc tccataccga cgatctgcga cctggcgcgc acgtttgccc
     6121 gggagatggg ggaggctaac tgaggatccc acgtcactat tgtatactct atattatact
     6181 ctatgttata ctctgtaatc ctactcaata aacgtgtcac gcctgtgaaa ccgtactaag
     6241 tctcccgtgt cttcttatca ccatcaggtg acatcctcgc ccaggctgtc aatcatgccg
     6301 gtatcgattc cagtagcacc ggccccacgc tgacaaccca ctcttgcagc gttagcagcg
     6361 cccctcttaa caagccgacc cccaccagcg tcgcggttac taacactcct ctccccgacc
     6421 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6481 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6541 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6601 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6661 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6721 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6781 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6841 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6901 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6961 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7021 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7081 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7141 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7201 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7261 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7321 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7381 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7441 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7501 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7561 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7621 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7681 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7741 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7801 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7861 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7921 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7981 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8041 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8101 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8161 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8221 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8281 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8341 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8401 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8461 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8521 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8581 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     8641 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     8701 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     8761 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     8821 gcatcagagc agattgtact gagagtgcac catatgggcc ggcccttaag gtttaaactc
     8881 atatttttcc ataaactgga cacactgaca gaaatgtttc cctaaaacat acttctctgt
     8941 tctagccaac ctattctcct ataatagtgt ttgtttttag aagggttgat aggattgctt
     9001 aggctaggta actgccacat agtctgtgtc ttctgcttgt cgtatgattt gaattgttta
     9061 actcatcggg caggccctta ttcccttatc ctcaaggaaa tatgtttgga ggagctctga
     9121 aggaaaagcg tgggaaaagg gacaagagtg agggaggaat gtctatctca ctcttgcccc
     9181 tatgttttac cttcatggtc aagtttcaaa ttgtcatcta gtaaggaaat gttttttaga
     9241 aaatgatcat caatgatttt tcctttgagc tgtcaacact tctgtgagcc taaatttaag
     9301 ttttcctggt ttgagcatgt gatttttggt tcataggaag attgagattt agtcttaaaa
     9361 gcatataaat gaccttaagt acaagcgcta aaagttggat ttgtagactt aataatacat
     9421 acatcttgga tttgatttta agtaatttta ctgtgtttta aatactaaca ggttgctctc
     9481 aagggagtgg tattttacac tatgctcatg gcgacagtca gcaaactcac ctgttgaagc
     9541 aaggtaagaa tgaagcattg gagcatactg ttctttttcc ttttcctatc ttaaacatac
     9601 attttttaaa tgtgcaggaa gaagctccat gggcactggt ctcagtggtg ggaaacgtcc
     9661 tagtcaagaa gaagacacac agagtattgg tcctaaagtc cagagacaga gcactaatag
     9721 cggccgc
//
