LOCUS       sequence-384542-        4753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM63
FEATURES             Location/Qualifiers
     source          1..4753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2640..2739)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2796..2812)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2796..2812)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2809..2831)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2984..3572)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3064..3083)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3708..4523)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3708..4523)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4431..4450
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4515..4534
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     4644..4660
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4644..4660
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      4661..4707
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat gtgtgtctgt tcttgtcaag aagctgtgga
      301 tatacgaggc ttcttcacgt ttattgcaaa gcagtgcatt gagcatgaga gtatgaagga
      361 tttcttcacc catgctgagg atgtagctta cactgcagca tacctatgtt tcttaggctt
      421 gaacggcact atggatactg atatctctaa atttctgaaa aggataagtc cttttagccc
      481 tgatgtgagg cagatttatc tgagtgtctt gatggggtta aattcattca gatcagagac
      541 tacaatgaat gccaaatata tgtttgattt tgttagtgcc ctccgtaatg atctggaaga
      601 gctactaagc aatgttcatc aaagtccgtg cattcgtctc caacaaggac tttcacacct
      661 ttctagattt ctctgggaca taaaatcaga tccatttcca cttggagaat tgatttctct
      721 tcaatcacag atgcaagctg tggccattga ggcagcgatt gcaatctaca cctcatatga
      781 tgagaaagtg aaaatgaaga ctactgaaac agaccatttg ccttttattt tgcaactgaa
      841 gttaaatcat gtcaaggtag aagtctccct gattaagcta ctgaaaagta aacccactgt
      901 aatagctcct ctaaaagatc tgattgattg tgttcgggaa gagctgatat tcatgaattt
      961 ttttttcatg gatgcatttg agcagtgcaa agagcaaact aacgtcactg aatttttcaa
     1021 tcttattcaa tctgtaacta gccaagcatg gttggtcatt tattctcttt atcgtgacat
     1081 aaagaaagaa ggcttagcca gggaaattag ttggtcacat tttcagttgc ttcttaagtt
     1141 caggtttatt agggcagtga ttggacagat atgtcccaga atttctgtgt catcaatact
     1201 ggaccatccc acaataaatc tgttgaactt tcttcctctc aactttaagg tccttgattc
     1261 ttatttcagc atgctaaaat catcaacgat agattcttcc gatagcccga tgatggattt
     1321 ggttttgatg gggtttcatg aatatattct tgacaacctg caactgaaga atgaaaccag
     1381 tttgacattt actaatacaa atgaggttaa aaagttttac tatggattgt tgctcctggt
     1441 aacatatctt tttgatcctc tagttcagtg tagtggattt ggaactatcg aaattgaggc
     1501 tgctggtgat agtaacagaa atagaagggt cagtcttgtt cttcagtatt tgactgtagc
     1561 tttaaagctt attgagcctg aggaaacctt gatggatatg ctaaccaagg ccacattgaa
     1621 agctccaata ctggatatgg ttgaaagtgc tcatgaagag cttattttcc tcagagcttt
     1681 tctaatggat gttctcaggc aacagatgaa gcttaacaaa tatcatcatc tcttgaggaa
     1741 tgttgaggag actgccaaca agttagcaca actcagtcgt tttcgtaatg aaagattcat
     1801 ggacgaaaac agcacctggg aagtgtgtct tatattgtct gatttcctat atgatattaa
     1861 gtttatcaag gtagaaatta gaaaggtatg cattcaggtc ttggatgcat cacttatgac
     1921 tttgccagat atcaaaggcc ttttaaactt tttatcaaac cgcctggaca cagtgcttga
     1981 ctgtgatgct ggttcgattg cttttctgat gaatcgaagt agagttctca aagagaaact
     2041 tatgtacttg ggacactttc ttgcagatgt tgtacaacac tacaatatgc atcaaggact
     2101 caaagacctc atgaaacatg ttctagatac caagtatgtc tgtttattgt ctgttgggga
     2161 ttataaatct gcttggtatt acatgttatc tctctccgat atgaagcaat tgcttaagtt
     2221 tgttgagaca gaggtcaaaa caattattcc taaagctcca gagtcatcaa gttatagctt
     2281 ccccaagaca aatggattag gatttcttca attttttcta ggaaacttgg aggagctgtt
     2341 atgttctaag ctcgattccg ttattgactg gaaggatcag attggatcag tcaaggaagg
     2401 catactgtgt ctaagagatc atttcgctga gaactttgat gagctagatg aagtttatgg
     2461 ttttatattg aaggtcactg aaatggcata taaagcagag tttgtcattg actcctgctt
     2521 agtcagttct catccactct ggtacaaatc tcatcgaatg tctgaagttc ttaagaatat
     2581 caagctttta aataaagctg ttactgagac ttatgaaaga aaggagatag atgtgataga
     2641 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     2701 cgaacaggtc actatcagtc aaaataaaat cattatttga ggctaggtgg aggctcagtg
     2761 atgataagtc tgcgatggtg gatgcatgtg tcatggtcat agctgtttcc tgtgtgaaat
     2821 tgttatccgc tcagagggca caatcctatt ccgcgctatc cgacaatctc caagacatta
     2881 ggtggagttc agttcggcgt atggcatatg tcgctggaaa gaacatgtga gcaaaaggcc
     2941 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3001 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3061 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3121 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3181 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3241 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3301 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3361 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3421 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3481 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3541 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3601 ctgacgctct attcaacaaa gccgccgtcc cgtcaagtca gcgtaaatgg gtagggggct
     3661 tcaaatcgtc cgctctgcca gtgttacaac caattaacaa attctgatta gaaaaactca
     3721 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga
     3781 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga
     3841 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc
     3901 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag
     3961 aatggcaaaa gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg
     4021 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga
     4081 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc
     4141 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc
     4201 tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg
     4261 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc
     4321 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca
     4381 tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc
     4441 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct cgagcaagac
     4501 gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt
     4561 tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac
     4621 acaacgtggc tttcccccgc cgctctagaa ctagtggatc caaataaaac gaaaggctca
     4681 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg cattatacga gacgtccagg
     4741 ttgggatacc tga
//
