LOCUS       sequence-384543-        4135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#50.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM68
FEATURES             Location/Qualifiers
     source          1..4135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2022..2121)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2178..2194)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2178..2194)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2191..2213)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2366..2954)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2446..2465)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3090..3905)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3090..3905)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3813..3832
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3897..3916
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     4026..4042
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4026..4042
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      4043..4089
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat gggatacatg gcatggcgcc cagtctgcaa
      301 taaggatgat gtattgtacc taggcgtaat aattatattg ataatggcag gacctcttgg
      361 ctactatagt tgtgcagacg tgctttctca agacttaatc aaacttatac acaagagttg
      421 gatgaaaagg atagagttca caccaatgta tgcatcgcca caagaagatt gtcgacaagt
      481 gaagagatgc attgaattag tattgagatg catggacgct gacaaaaata aaagacccac
      541 tgtaaaggat atagtccgtg agttgaaaca gacagaaaca gcgtcgccct gggtccaggc
      601 taagttcgcc aagattgggc agtggggagg aattggaggg ggcccacgtg acattgagat
      661 ggcaccccac cgtctgaaaa gcttgacaat cggtagtggc aaggttatat attcgcttga
      721 attttcgtac gatgaccatc atgagaagaa ccacataaca ggcccatggg gcggtcatgg
      781 acctgaaggt cacgggaaca aatttcgtat tgtcctccag cctaccgagt ttctaaccgt
      841 agtttccgga acaatcggtc catatgactc cgcaccggcc ggtgttataa agtcacttac
      901 ttttatcact accgctggtc ggtatggacc ttttggagaa gaaaagggaa ctcctttcca
      961 gatcccagtg cagagcaatg ggagcatcgt aggcttcttt gcacgtgcag ggtggtactt
     1021 agatgcattc ggcatctatg tgaaacctat gcaggaaacc gaggaggctg gccttgccaa
     1081 gatcggacca tggggcggaa atggaggaca agtgcgcgac ctcaagatgc caccacaacg
     1141 tctagaaagc gtgacaattt gcagtggcac ggtgcttaat tctcttacat tcatttacca
     1201 tggccatgat ggaaagaagc acaccgctgg tccgtggggc gcatctactg ggaatcctga
     1261 tcaaactgat acaattctgc tttgcagttc ggagttcctg acaggggtcc gtggaacaac
     1321 cggaccattt gaccctgcat cgtctgacgt tatcgtcacg tcgcttactc taatcacgaa
     1381 cacccatagc tatggtcctt tcgggcaagg cagaggaacc tctttccaga ttccattgtg
     1441 gggcaagggc agcatcgtag gctttttcgg gtgttcggag tcatacatta atgcgatcgg
     1501 tgtctatgtg aatccttatc tggaagcaat gcagaaggag ttggttagct ctcgtgagcc
     1561 tcgacttaca aagatcgggc cgtggggtgg gaatggagac aaagggaatg ttcggtacat
     1621 caacgctaag atggaacccc agcatctgga gagcgtaaca gttagctttg gctccgtcat
     1681 caattcactc tcattttcat acatcgactt taagggggtt aagcacaatg taggtccttg
     1741 gggcactccc tccgggaatt catacaagat tgtgctggaa tcgtcggagg tactgcaggg
     1801 ggtttccggc acggtgggtt cgtctgatgg tacatcttcc aaccttgtaa catcgctcac
     1861 gtttgcgacg gaccaagccc gtgcctatgg accttttgga gcaggaggag gtactccgtt
     1921 cagcgctgcg gcggcggcgg ggaatgacgg ccctgtcgtt gccttcttcg ggcgtgcagg
     1981 gttatgtctt gaagcacttg gggtctacgt ccacagatac gacccagctt tcttgtacaa
     2041 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
     2101 tcaaaataaa atcattattt gaggctaggt ggaggctcag tgatgataag tctgcgatgg
     2161 tggatgcatg tgtcatggtc atagctgttt cctgtgtgaa attgttatcc gctcagaggg
     2221 cacaatccta ttccgcgcta tccgacaatc tccaagacat taggtggagt tcagttcggc
     2281 gtatggcata tgtcgctgga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2341 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2401 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2461 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2521 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2581 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2641 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2701 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2761 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     2821 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2881 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2941 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct ctattcaaca
     3001 aagccgccgt cccgtcaagt cagcgtaaat gggtaggggg cttcaaatcg tccgctctgc
     3061 cagtgttaca accaattaac aaattctgat tagaaaaact catcgagcat caaatgaaac
     3121 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     3181 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     3241 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     3301 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     3361 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     3421 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     3481 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     3541 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     3601 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     3661 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     3721 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     3781 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     3841 tcagcatcca tgttggaatt taatcgcggc ctcgagcaag acgtttcccg ttgaatatgg
     3901 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     3961 atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttccccc
     4021 gccgctctag aactagtgga tccaaataaa acgaaaggct cagtcgaaag actgggcctt
     4081 tcgttttatc tgttgtttgt cgcattatac gagacgtcca ggttgggata cctga
//
