LOCUS       sequence-384545-       11759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone
            with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11759)
  AUTHORS   Girish V, Lakhani AA, Thompson SL, Scaduto CM, Brown LM, Hagenson 
            RA, Sausville EL, Mendelson BE, Kandikuppa PK, Lukow DA, Yuan ML, 
            Stevens EC, Lee SN, Schukken KM, Akalu SM, Vasudevan A, Zou C, 
            Salovska B, Li W, Smith JC, Taylor AM, Martienssen RA, Liu Y, Sun R,
            Sheltzer JM
  TITLE     Oncogene-like addiction to aneuploidy in human cancers.
  JOURNAL   Science. 2023 Jul 6:eadg4521. doi: 10.1126/science.adg4521.
  PUBMED    37410869
REFERENCE   2  (bases 1 to 11759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2023 Jul 6:eadg4521. doi: 10.1126/science.adg4521."
COMMENT     Sequence Label: OR6K6_Deletion_gRNA3
FEATURES             Location/Qualifiers
     source          1..11759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     135..154
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     388..405
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    522..543
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(558..563,564..581,582..588)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    596..612
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     601..623
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     620..636
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     620..636
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     655..675
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        657..675
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        703..929
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             930..1110
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1157..1282
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1775..2008
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     promoter        complement(2602..2850)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(2660..2679)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(2830..2850)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    2857..2974
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3043..3254
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      3274..3283
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3283..3306
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3313..3333
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3358..7458
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3358..7458
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7459..7506
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7522..7578
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7579..7581,7582..7584,7585..8298)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7579..7581,7582..7584,7585..8295)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7885..7904)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8232..8253
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8314..8902
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8367..8387)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8785..8796)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8974..9207
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9285..9406
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9322..9341)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9376..9395
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9446..9581
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9601..9620)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9602..9620)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9630..9647)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9630..9646)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9639..9661)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9788..10243
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9875..9894)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10085..10106
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10269..10373
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10374..10442,10443..11234)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10592..10611)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(11405..11759,1..234)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
       61 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      121 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      181 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      241 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      301 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      361 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      421 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      481 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
      541 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
      601 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
      661 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
      721 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
      781 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
      841 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
      901 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
      961 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     1021 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     1081 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     1141 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     1201 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     1261 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     1321 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     1381 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     1441 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     1501 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     1561 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     1621 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1681 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1741 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     1801 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     1861 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     1921 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     1981 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcgcca
     2041 tttgtctcaa gatctagaat tcaaaaaaag caccgactcg gtgccacttt gctgtttcca
     2101 gcaaagttga taacggacta gccttattta aacttgctat gctgtttcca gcatagctct
     2161 taaactcacg tgattgtggc atcatcgata aacatggcct ctcaagaact atataaagct
     2221 aagaatagaa aaaattttac agttatggtg actaaactgt aacccttttt taagtttcaa
     2281 agtatataat aagttatgac cttttgtacc acacacatta tcatcatata gtttgattag
     2341 ggctaagttt aatagtctcc ataaccttat tacatcatat atgcaaatga agggaaccat
     2401 gggaaattgg ccctaggccc gcccgacctc tgcgcgtctg tctagtggac tgcgcgggca
     2461 gacaagctcg gtcacgcaaa aaaagcaccg actcggtgcc actttgctgt ttccagcaaa
     2521 gttgataacg gactagcctt atttcaactt gctatgcact cttgtgcata gctctgaaac
     2581 cctggtcatt aggattccgc cggtgtttcg tcctttccac aagatatata aagccaagaa
     2641 atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac ataattttaa
     2701 aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta ctaatatctt
     2761 tgtgtttaca gtcaaattaa ttctaattat ctctctaaca gccttgtatc gtatatgcaa
     2821 atatgaagga atcatgggaa ataggccctc ccacaatttt aaaagaaaag gggggattgg
     2881 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     2941 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     3001 tccactttgg cgccggctcg agtggctccg gtgcccgtca gtgggcagag cgcacatcgc
     3061 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
     3121 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     3181 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     3241 ccgccagaac acaggtgtcg tgacgcggga tccgccacca tggattacaa agacgatgac
     3301 gataagatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgac
     3361 aagaagtaca gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc
     3421 gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc
     3481 atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc
     3541 cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg
     3601 caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa
     3661 gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc
     3721 gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg
     3781 gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc
     3841 aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac
     3901 aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac
     3961 gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg
     4021 gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt
     4081 gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc
     4141 aaactgcagc tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc
     4201 ggcgaccagt acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg
     4261 agcgacatcc tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc
     4321 aagagatacg acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag
     4381 ctgcctgaga agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac
     4441 attgacggcg gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag
     4501 atggacggca ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag
     4561 cggaccttcg acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt
     4621 ctgcggcggc aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag
     4681 atcctgacct tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc
     4741 gcctggatga ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg
     4801 gacaagggcg cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg
     4861 cccaacgaga aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac
     4921 gagctgacca aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc
     4981 gagcagaaaa aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag
     5041 cagctgaaag aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc
     5101 gtggaagatc ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag
     5161 gacaaggact tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc
     5221 ctgacactgt ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg
     5281 ttcgacgaca aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg
     5341 agccggaagc tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc
     5401 ctgaagtccg acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg
     5461 acctttaaag aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag
     5521 cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag
     5581 gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa
     5641 atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag
     5701 cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa
     5761 aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg
     5821 tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg
     5881 cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag
     5941 aaccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac
     6001 tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag
     6061 gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg
     6121 gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag
     6181 tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg
     6241 gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac
     6301 cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct
     6361 aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc
     6421 gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc
     6481 atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg
     6541 atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc
     6601 gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca
     6661 ggcggcttca gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga
     6721 aagaaggact gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct
     6781 gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag
     6841 ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg
     6901 gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc
     6961 ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag
     7021 ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat
     7081 gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac
     7141 aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg
     7201 gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc
     7261 agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc
     7321 gccttcaagt actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg
     7381 ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg
     7441 tctcagctgg gaggcgacaa gcgtcctgct gctactaaga aagctggtca agctaagaaa
     7501 aagaaagcta gcggcagcgg cgccaccaac ttcagcctgc tgaagcaggc cggcgacgtg
     7561 gaggagaacc ccggccccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     7621 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     7681 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     7741 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     7801 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     7861 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     7921 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     7981 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     8041 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     8101 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     8161 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     8221 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     8281 gacgagctgt acaagtagac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     8341 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     8401 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     8461 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     8521 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     8581 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     8641 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     8701 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     8761 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     8821 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     8881 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     8941 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     9001 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     9061 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     9121 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     9181 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     9241 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     9301 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9361 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     9421 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     9481 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     9541 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     9601 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     9661 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     9721 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     9781 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     9841 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     9901 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     9961 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
    10021 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
    10081 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
    10141 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
    10201 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
    10261 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
    10321 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
    10381 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
    10441 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
    10501 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
    10561 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
    10621 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
    10681 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
    10741 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
    10801 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
    10861 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
    10921 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    10981 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    11041 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    11101 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    11161 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    11221 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    11281 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    11341 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    11401 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    11461 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    11521 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    11581 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    11641 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    11701 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcga
//
