LOCUS       sequence-384548-       11759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone
            with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11759)
  AUTHORS   Girish V, Lakhani AA, Thompson SL, Scaduto CM, Brown LM, Hagenson 
            RA, Sausville EL, Mendelson BE, Kandikuppa PK, Lukow DA, Yuan ML, 
            Stevens EC, Lee SN, Schukken KM, Akalu SM, Vasudevan A, Zou C, 
            Salovska B, Li W, Smith JC, Taylor AM, Martienssen RA, Liu Y, Sun R,
            Sheltzer JM
  TITLE     Oncogene-like addiction to aneuploidy in human cancers.
  JOURNAL   Science. 2023 Jul 6:eadg4521. doi: 10.1126/science.adg4521.
  PUBMED    37410869
REFERENCE   2  (bases 1 to 11759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2023 Jul 6:eadg4521. doi: 10.1126/science.adg4521."
COMMENT     Sequence Label: OR6K6_Deletion_gRNA2
FEATURES             Location/Qualifiers
     source          1..11759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..25
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7..25
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        53..279
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             280..460
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    507..632
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1125..1358
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     promoter        complement(1952..2200)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(2010..2029)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(2180..2200)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    2207..2324
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2393..2604
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2624..2633
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2633..2656
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2663..2683
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2708..6808
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2708..6808
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6809..6856
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6872..6928
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(6929..6931,6932..6934,6935..7648)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6929..6931,6932..6934,6935..7645)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7235..7254)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7582..7603
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7664..8252
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7717..7737)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8135..8146)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8324..8557
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8635..8756
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8672..8691)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8726..8745
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8796..8931
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8951..8970)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8952..8970)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8980..8997)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8980..8996)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8989..9011)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9138..9593
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9225..9244)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9435..9456
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9619..9723
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9724..9792,9793..10584)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9942..9961)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10755..11343
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11244..11263
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11497..11514
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11631..11652
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11667..11672,11673..11690,11691..11697)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11705..11721
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11710..11732
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11729..11745
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11729..11745
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
       61 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
      121 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
      181 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
      241 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
      301 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      361 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      421 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
      481 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
      541 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
      601 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
      661 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
      721 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
      781 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
      841 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
      901 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
      961 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1021 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1081 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     1141 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     1201 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     1261 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     1321 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     1381 aaactcgcca tttgtctcaa gatctagaat tcaaaaaaag caccgactcg gtgccacttt
     1441 gctgtttcca gcaaagttga taacggacta gccttattta aacttgctat gctgtttcca
     1501 gcatagctct taaaccggga atggctgggt ataatcgata aacatggcct ctcaagaact
     1561 atataaagct aagaatagaa aaaattttac agttatggtg actaaactgt aacccttttt
     1621 taagtttcaa agtatataat aagttatgac cttttgtacc acacacatta tcatcatata
     1681 gtttgattag ggctaagttt aatagtctcc ataaccttat tacatcatat atgcaaatga
     1741 agggaaccat gggaaattgg ccctaggccc gcccgacctc tgcgcgtctg tctagtggac
     1801 tgcgcgggca gacaagctcg gtcacgcaaa aaaagcaccg actcggtgcc actttgctgt
     1861 ttccagcaaa gttgataacg gactagcctt atttcaactt gctatgcact cttgtgcata
     1921 gctctgaaac tcgtcatgac taccaaaatc cggtgtttcg tcctttccac aagatatata
     1981 aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac
     2041 ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta
     2101 ctaatatctt tgtgtttaca gtcaaattaa ttctaattat ctctctaaca gccttgtatc
     2161 gtatatgcaa atatgaagga atcatgggaa ataggccctc ccacaatttt aaaagaaaag
     2221 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2281 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2341 acagcagaga tccactttgg cgccggctcg agtggctccg gtgcccgtca gtgggcagag
     2401 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     2461 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     2521 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     2581 aacgggtttg ccgccagaac acaggtgtcg tgacgcggga tccgccacca tggattacaa
     2641 agacgatgac gataagatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc
     2701 agcagccgac aagaagtaca gcatcggcct ggacatcggc accaactctg tgggctgggc
     2761 cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga
     2821 ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc
     2881 cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat
     2941 ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca
     3001 cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt
     3061 cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag
     3121 aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc
     3181 ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag
     3241 cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa
     3301 ccccatcaac gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag
     3361 cagacggctg gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg
     3421 aaacctgatt gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc
     3481 cgaggatgcc aaactgcagc tgagcaagga cacctacgac gacgacctgg acaacctgct
     3541 ggcccagatc ggcgaccagt acgccgacct gtttctggcc gccaagaacc tgtccgacgc
     3601 catcctgctg agcgacatcc tgagagtgaa caccgagatc accaaggccc ccctgagcgc
     3661 ctctatgatc aagagatacg acgagcacca ccaggacctg accctgctga aagctctcgt
     3721 gcggcagcag ctgcctgaga agtacaaaga gattttcttc gaccagagca agaacggcta
     3781 cgccggctac attgacggcg gagccagcca ggaagagttc tacaagttca tcaagcccat
     3841 cctggaaaag atggacggca ccgaggaact gctcgtgaag ctgaacagag aggacctgct
     3901 gcggaagcag cggaccttcg acaacggcag catcccccac cagatccacc tgggagagct
     3961 gcacgccatt ctgcggcggc aggaagattt ttacccattc ctgaaggaca accgggaaaa
     4021 gatcgagaag atcctgacct tccgcatccc ctactacgtg ggccctctgg ccaggggaaa
     4081 cagcagattc gcctggatga ccagaaagag cgaggaaacc atcaccccct ggaacttcga
     4141 ggaagtggtg gacaagggcg cttccgccca gagcttcatc gagcggatga ccaacttcga
     4201 taagaacctg cccaacgaga aggtgctgcc caagcacagc ctgctgtacg agtacttcac
     4261 cgtgtataac gagctgacca aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt
     4321 cctgagcggc gagcagaaaa aggccatcgt ggacctgctg ttcaagacca accggaaagt
     4381 gaccgtgaag cagctgaaag aggactactt caagaaaatc gagtgcttcg actccgtgga
     4441 aatctccggc gtggaagatc ggttcaacgc ctccctgggc acataccacg atctgctgaa
     4501 aattatcaag gacaaggact tcctggacaa tgaggaaaac gaggacattc tggaagatat
     4561 cgtgctgacc ctgacactgt ttgaggacag agagatgatc gaggaacggc tgaaaaccta
     4621 tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag cggcggagat acaccggctg
     4681 gggcaggctg agccggaagc tgatcaacgg catccgggac aagcagtccg gcaagacaat
     4741 cctggatttc ctgaagtccg acggcttcgc caacagaaac ttcatgcagc tgatccacga
     4801 cgacagcctg acctttaaag aggacatcca gaaagcccag gtgtccggcc agggcgatag
     4861 cctgcacgag cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca
     4921 gacagtgaag gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat
     4981 cgtgatcgaa atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga
     5041 gagaatgaag cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca
     5101 ccccgtggaa aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg
     5161 gcgggatatg tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga
     5221 ccatatcgtg cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag
     5281 aagcgacaag aaccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat
     5341 gaagaactac tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa
     5401 tctgaccaag gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag
     5461 acagctggtg gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat
     5521 gaacactaag tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa
     5581 gtccaagctg gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa
     5641 caactaccac cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa
     5701 aaagtaccct aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg
     5761 gaagatgatc gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta
     5821 cagcaacatc atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa
     5881 gcggcctctg atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga
     5941 ttttgccacc gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga
     6001 ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc aagaggaaca gcgataagct
     6061 gatcgccaga aagaaggact gggaccctaa gaagtacggc ggcttcgaca gccccaccgt
     6121 ggcctattct gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag
     6181 tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat
     6241 cgactttctg gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc
     6301 taagtactcc ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccggcga
     6361 actgcagaag ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc
     6421 cagccactat gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt
     6481 ggaacagcac aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag
     6541 agtgatcctg gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga
     6601 taagcccatc agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg
     6661 agcccctgcc gccttcaagt actttgacac caccatcgac cggaagaggt acaccagcac
     6721 caaagaggtg ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg
     6781 gatcgacctg tctcagctgg gaggcgacaa gcgtcctgct gctactaaga aagctggtca
     6841 agctaagaaa aagaaagcta gcggcagcgg cgccaccaac ttcagcctgc tgaagcaggc
     6901 cggcgacgtg gaggagaacc ccggccccat ggtgagcaag ggcgaggagc tgttcaccgg
     6961 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     7021 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     7081 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     7141 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     7201 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     7261 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     7321 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     7381 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     7441 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     7501 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     7561 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     7621 tctcggcatg gacgagctgt acaagtagac gcgttaagtc gacaatcaac ctctggatta
     7681 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     7741 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     7801 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     7861 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     7921 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     7981 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     8041 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     8101 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     8161 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     8221 gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag
     8281 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     8341 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     8401 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     8461 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8521 ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat
     8581 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     8641 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8701 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8761 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     8821 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     8881 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     8941 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     9001 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     9061 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     9121 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     9181 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     9241 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     9301 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     9361 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     9421 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     9481 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     9541 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     9601 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     9661 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     9721 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     9781 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     9841 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     9901 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     9961 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
    10021 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
    10081 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
    10141 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
    10201 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
    10261 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    10321 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    10381 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    10441 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    10501 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    10561 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    10621 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    10681 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    10741 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    10801 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    10861 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    10921 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    10981 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    11041 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    11101 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    11161 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    11221 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    11281 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    11341 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    11401 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    11461 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    11521 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    11581 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
    11641 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
    11701 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaa
//
