LOCUS       sequence-384551-        5839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ASAP3ER under control of human synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Campbell EP, Abushawish AA, Valdez LA, Bell MK, Haryono M, Rangamani
            P, Bloodgood BL
  TITLE     Electrical signals in the ER are cell type and stimulus specific 
            with extreme spatial compartmentalization in neurons.
  JOURNAL   Cell Rep. 2023 Jan 31;42(1):111943. doi: 
            10.1016/j.celrep.2022.111943. Epub 2023 Jan 5.
  PUBMED    36640310
REFERENCE   2  (bases 1 to 5839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-01-31"; volume: "42(1):111943. doi"; pages: " 
            10.1016/j.celrep.2022.111943. Epub 2023 Jan 5"
COMMENT     Sequence Label: AAV-hSyn-ASAP3ER
FEATURES             Location/Qualifiers
     source          1..5839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..579
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      612..621
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1923..2511
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1976..1996)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2394..2405)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2543..3019
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2742..2761)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3059..3199
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3059..3188
                     /label=AAV2 ITR
     rep_origin      3274..3729
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3361..3380)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3571..3592
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3746..3765)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3865..3887
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3925..3943)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4011..4115
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4116..4184,4185..4976)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4334..4353)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5147..5735
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5636..5655
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   join(5797..5839,1..87)
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 ggcgtcgggc gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag
       61 tggccaactc catcactagg ggttcctgcg gccgcacgcg tgtgtctaga ctgcagaggg
      121 ccctgcgtat gagtgcaagt gggttttagg accaggatga ggcggggtgg gggtgcctac
      181 ctgacgaccg accccgaccc actggacaag cacccaaccc ccattcccca aattgcgcat
      241 cccctatcag agagggggag gggaaacagg atgcggcgag gcgcgtgcgc actgccagct
      301 tcagcaccgc ggacagtgcc ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc
      361 actgaaggcg cgctgacgtc actcgccggt cccccgcaaa ctccccttcc cggccacctt
      421 ggtcgcgtcc gcgccgccgc cggcccagcc ggaccgcacc acgcgaggcg cgagataggg
      481 gggcacgggc gcgaccatct gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg
      541 gtgggcagcg gaggagtcgt gtcgtgcctg agagcgcagt cgagaaggta ccggatctgc
      601 atgagctagc cgccaccatg gagacgactg tgaggtatga acaggggtca gagctcacta
      661 aaacttcgag ctctccaaca gcagatgagc ccacgataaa gattgatgat ggtcgtgatg
      721 agggtaatga acaagacagc tgttccaata ccattaggag aaaaatttcc ccgtttgtga
      781 tgtcatttgg attcagagta tttggagttg tgcttatcat tgtagacatc atagtggtga
      841 ttgtggatct ggccatcagt gagaagaaaa gaggcattag agagattctt gaaggtgttt
      901 ccctggctat agcactcttc ttccttgttg atgttctcat gagagtgttt gttgaaggct
      961 tcaagaacta tttccggtcc aaactgaata ctttggatgc agtcatagta gtgggcactc
     1021 tgctaattaa tatgacctac tccttctctg acggtacatt tcgcggtgat aacgtgtata
     1081 ttaccgcgga taaacagaaa aacggcatta aagcgaactt taccgtgcgc cataacgtgg
     1141 aagatggcag cgtgcagctg gcggatcatt atcagcagaa caccccgatt ggcgatggcc
     1201 cggtgctgct gccggataac cattatctga gcacccagac cgtgctgagc aaagatccga
     1261 acgaaaaacg cgatcacatg gtgctgctgg aatttgtgac cgcagcgggc attacacacg
     1321 gcatggatga actgtatggc ggcactggcg gcagcgcgag ccagggcgaa gaactgttta
     1381 ccggcgtggt gccgattctg gtggaactgg atggcgatgt gaacggccat aaatttagcg
     1441 tgcgcggcga aggcgaaggc gatgcgacca ttggcaaact gaccctgaaa tttatttgca
     1501 ccaccggcaa actaccggtg ccgtggccga ccctggtgac caccttaacc tatggcgtgc
     1561 agtgctttag ccgctatccg gatcatatga aacgccatga tttttttaaa agcgcgatgc
     1621 cggaaggcta tgtgcaggaa cgcaccatta gctttaaaga tgatggcaaa tataaaaccc
     1681 gcgcggtggt gaaatttgaa ggcgataccc tggtgaaccg cattgaactg aaaggcaccg
     1741 attttaaaga agatggcaac attctggggc ataaactgga atataacaca gatcagatgc
     1801 cgcagatggt tactcttctt cgagttctga gaattgttat cttaataaga atatttcagc
     1861 tggcttcaca gaagaaacaa cttgaagtgg taaccaagaa ggcggcgtaa aagcttatcg
     1921 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     1981 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     2041 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     2101 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     2161 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     2221 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     2281 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     2341 tcgcctatgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     2401 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     2461 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     2521 gagcgctgct cgagagatct acgggtggca tccctgtgac ccctccccag tgcctctcct
     2581 ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat
     2641 cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag
     2701 caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg
     2761 gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct
     2821 cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt
     2881 tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta
     2941 atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac
     3001 tgctcccttc cctgtccttc tgattttgta ggtaaccacg tgcggaccga gcggccgcag
     3061 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     3121 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     3181 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     3241 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
     3301 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg
     3361 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     3421 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     3481 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     3541 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     3601 tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt
     3661 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     3721 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     3781 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     3841 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     3901 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     3961 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4021 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4081 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4141 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4201 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4261 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4321 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     4381 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     4441 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     4501 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     4561 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     4621 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     4681 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     4741 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     4801 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     4861 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     4921 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     4981 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5041 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5101 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5161 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5221 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5281 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5341 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5401 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5461 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5521 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5581 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5641 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5701 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5761 ggttcctggc cttttgctgg ccttttgctc acatgtcctg caggcagctg cgcgctcgct
     5821 cgctcactga ggccgcccg
//
