LOCUS       sequence-384553-       11001 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mGluR1-IRES-cyRFP1 under the control of the pCAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11001)
  AUTHORS   Campbell EP, Abushawish AA, Valdez LA, Bell MK, Haryono M, Rangamani
            P, Bloodgood BL
  TITLE     Electrical signals in the ER are cell type and stimulus specific 
            with extreme spatial compartmentalization in neurons.
  JOURNAL   Cell Rep. 2023 Jan 31;42(1):111943. doi: 
            10.1016/j.celrep.2022.111943. Epub 2023 Jan 5.
  PUBMED    36640310
REFERENCE   2  (bases 1 to 11001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-01-31"; volume: "42(1):111943. doi"; pages: " 
            10.1016/j.celrep.2022.111943. Epub 2023 Jan 5"
COMMENT     Sequence Label: pCAG-Myc-GRM1-IRES-CyRFP1
FEATURES             Location/Qualifiers
     source          1..11001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             128..157
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    3671..4244
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3838..3855)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4065..4084
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      4245..4254
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5031..5048)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5037..5261
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5307..5735
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5394..5413)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5604..5625
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5744..5764)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5749..6078
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5929..6064
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5991..6010
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6089..6688
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6089..6108)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6585..6605
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6848..6969
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6885..6904)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6939..6958
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7018..7034)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7018..7034)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7031..7053)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7042..7058
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7066..7072,7073..7090,7091..7096))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7111..7132
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7249..7266)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7420..8008)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7500..7519)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8179..8970,8971..9039))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8802..8821
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9040..9144)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        9176..9555
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          9833..10849
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     10773..10795
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     10857..10876
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 gctagcgttt ttaactcgag ccaccatggt ccggctcctc ttgattttct tcccaatgat
       61 ctttttggag atgtccattt tgcccaggat gcctgacaga aaagtattgc tggcaggtgc
      121 ctcgtccgaa caaaaactca tctcagaaga ggatctgcag cgctccgtgg cgagaatgga
      181 cggagatgtc atcatcggag ccctcttctc agtccatcac cagcctccag ccgagaaggt
      241 acccgaaagg aagtgtgggg agatcaggga acagtatggt atccagaggg tggaggccat
      301 gttccacacg ttggataaga ttaacgcgga cccggtgctc ctgcccaaca tcactctggg
      361 cagtgagatc cgggactcct gctggcactc ttcagtggct ctcgaacaga gcatcgaatt
      421 catcagagac tccctgattt ccatccgaga tgagaaggat gggctgaacc gatgcctgcc
      481 tgatggccag accctgcccc ctggcaggac taagaagcct attgctggag tgatcggccc
      541 tggctccagc tctgtggcca ttcaagtcca gaatcttctc cagctgttcg acatcccaca
      601 gatcgcctat tctgccacaa gcatagacct gagtgacaaa actttgtaca aatacttcct
      661 gagggtggtc ccttctgaca ctttgcaggc aagggcgatg ctcgacatag tcaagcgtta
      721 caactggacc tatgtctcag cagtccacac agaagggaat tacggcgaga gtggaatgga
      781 tgctttcaaa gaactggctg cccaggaagg cctctgcatc gcacactcgg acaaaatcta
      841 cagcaatgct ggcgagaaga gctttgaccg gctcctgcgt aaactccggg agcggcttcc
      901 caaggccagg gttgtggtct gcttctgcga gggcatgaca gtgcggggct tactgagtgc
      961 catgcgccgc ctgggcgtcg tgggcgagtt ctcactcatt ggaagtgatg gatgggcaga
     1021 cagagatgaa gtcatcgaag gctatgaggt ggaagccaac ggagggatca caataaagct
     1081 tcagtctcca gaggtcaggt catttgatga ctacttcctg aagctgaggc tggacaccaa
     1141 cacaaggaat ccttggttcc ctgagttctg gcaacatcgc ttccagtgtc gcctacctgg
     1201 acacctcttg gaaaacccca actttaagaa agtgtgcaca ggaaatgaaa gcttggaaga
     1261 aaactatgtc caggacagca aaatgggatt tgtcatcaat gccatctatg ccatggcaca
     1321 tgggctgcag aacatgcacc atgctctgtg tcccggccat gtgggcctgt gtgatgctat
     1381 gaaacccatt gatggcagga agctcctgga tttcctcatc aaatcctctt ttgtcggagt
     1441 gtctggagag gaggtgtggt tcgatgagaa gggggatgct cccggaaggt atgacattat
     1501 gaatctgcag tacacagaag ctaatcgcta tgactatgtc cacgtgggga cctggcatga
     1561 aggagtgctg aatattgatg attacaaaat ccaaatgaac aaaagcggaa tggtacgatc
     1621 tgtgtgcagt gagccttgct taaagggtca gattaaggtc atacggaaag gagaagtgag
     1681 ctgctgctgg atctgcacgg cctgcaaaga gaatgagttt gtgcaggacg agttcacctg
     1741 cagagcctgt gacctggggt ggtggcccaa cgcagagctc acaggctgtg agcccattcc
     1801 tgtccgttat cttgagtgga gtgacataga atctatcata gccatcgcct tttcttgcct
     1861 gggcatcctc gtgacgctgt ttgtcaccct catcttcgtt ctgtaccggg acacacccgt
     1921 ggtcaaatcc tccagtaggg agctctgcta tatcattctg gctggtattt tcctcggcta
     1981 tgtgtgccct ttcaccctca tcgccaaacc tactaccaca tcctgctacc tccagcgcct
     2041 cctagttggc ctctcttctg ccatgtgcta ctctgcttta gtgaccaaaa ccaatcgtat
     2101 tgcacgcatc ctggctggca gcaagaagaa gatctgcacc cggaagccca gattcatgag
     2161 cgcttgggcc caagtgatca tagcctccat tctgattagt gtacagctaa cactagtggt
     2221 gaccttgatc atcatggagc ctcccatgcc cattttgtcc tacccgagta tcaaggaagt
     2281 ctaccttatc tgcaatacca gcaacctggg tgtagtggcc cctgtgggtt acaatggact
     2341 cctcatcatg agctgtacct actatgcctt caagacccgc aacgtgccgg ccaacttcaa
     2401 tgaggctaaa tacatcgcct tcaccatgta cactacctgc atcatctggc tggctttcgt
     2461 tcccatttac tttgggagca actacaagat catcactacc tgcttcgcgg tgagcctcag
     2521 tgtgacggtg gccctggggt gcatgtttac tccgaagatg tacatcatca ttgccaaacc
     2581 tgagaggaac gtccgcagtg ccttcacgac ctctgatgtt gtccgcatgc acgtcggtga
     2641 tggcaaactg ccgtgccgct ccaacacctt cctcaacatt ttccggagaa agaagcccgg
     2701 ggcagggaat gccaattcta acggcaagtc tgtgtcatgg tctgaaccag gtggaagaca
     2761 ggcgcccaag ggacagcacg tgtggcagcg cctctctgtg cacgtgaaga ccaacgagac
     2821 ggcctgtaac caaacagccg taatcaaacc cctcactaaa agttaccaag gctctggcaa
     2881 gagcctgacc ttttcagatg ccagcaccaa gaccctttac aatgtggaag aagaggacaa
     2941 taccccttct gctcacttca gccctcccag cagcccttct atggtggtgc accgacgcgg
     3001 gccacccgtg gccaccacac cacctctgcc accccatctg accgcagaag agacccccct
     3061 gttcctggct gattccgtca tccccaaggg cttgcctcct cctctcccgc agcagcagcc
     3121 acagcagccg ccccctcagc agcccccgca gcagcccaag tccctgatgg accagctgca
     3181 aggcgtagtc accaacttcg gttcggggat tccagatttc catgcggtgc tggcaggccc
     3241 ggggacacca ggaaacagcc tgcgctctct gtacccgccc ccgcctccgc cgcaacacct
     3301 gcagatgctg cccctgcacc tgagcacctt ccaggaggag tccatctccc ctcctgggga
     3361 ggacatcgat gatgacagtg agagattcaa gctcctgcag gagttcgtgt acgagcgcga
     3421 agggaacacc gaagaagatg aattggaaga ggaggaggac ctgcccacag ccagcaagct
     3481 gacccctgag gattctcctg ccctgacgcc tccttctcct ttccgagatt ccgtggcctc
     3541 tggcagctca gtgcccagtt cccccgtatc tgagtcggtc ctctgcaccc ctccaaatgt
     3601 gacctacgcc tctgtcattc tgagggacta caagcaaagc tcttccaccc tgtaggcggc
     3661 cgcgatatcg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     3721 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     3781 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     3841 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     3901 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     3961 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     4021 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     4081 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     4141 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     4201 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagccacc atggtgtcta
     4261 agggcgaaga gctgatcaag gagaacatga gaagcaagct gtacctggaa ggcagcgtga
     4321 acggccacca gttcaagtgc acccacgaag gggagggcaa gccctacgag ggcaagcaga
     4381 ccgcgaggat caaggtggtg gagggaggcc ccctgccgtt cgcattcgac atcctggcca
     4441 ccatgtttat gtacgggagc aaggtgttca tcaagtaccc cgccgacctc cccgattatt
     4501 ttaagcagtc cttccctgag ggcttcacat gggagagagt catggtgttc gaagacgggg
     4561 gcgtgctgac cgccacccag gacaccagcc tccaggacgg cgagctcatc tacaacgtca
     4621 agctcagagg ggtgaacttc ccagccaacg gccccgtgat gcagaagaaa acactgggct
     4681 gggagcccag caccgagacc atgacccccg ctgacggcgg cctggaaggc agatgcgaca
     4741 aggtgctgaa gctcgtgggc gggggccacc tgcacgtcaa cttcaagacc acatacaagt
     4801 ccaagaaacc cgtgaagatg cccggcgtcc actacgtgga ccgcagactg gaaagaatca
     4861 aggaggccga caacgagacc tacgtcgagc agtacgagca cgctgtggcc agatactcca
     4921 acctgggcgg aggcatggac gagctgtaca agtaataatg aaagcttggt accgagctcg
     4981 gatccactag tccagtgtgg tggaattctg cagataaacc cgctgatcag cctcgactgt
     5041 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     5101 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     5161 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     5221 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     5281 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     5341 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5401 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5461 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5521 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5581 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5641 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5701 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     5761 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5821 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5881 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     5941 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6001 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6061 ggcctaggct tttgcaaaaa gctcccgcat gaccgagtac aagcccacgg tgcgcctcgc
     6121 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     6181 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     6241 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     6301 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     6361 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     6421 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     6481 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     6541 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     6601 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     6661 gtgcatgacc cgcaagcccg gtgcctgatt cgaaatgacc gaccaagcga cgcccaacct
     6721 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     6781 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     6841 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6901 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6961 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     7021 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7081 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7141 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7201 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7261 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7321 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7381 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7441 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7501 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7561 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7621 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7681 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7741 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7801 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7861 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7921 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7981 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8041 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8101 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8161 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8221 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8281 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8341 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8401 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8461 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8521 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8581 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8641 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8701 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8761 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8821 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8881 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8941 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9001 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9061 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9121 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacat
     9181 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     9241 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     9301 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     9361 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     9421 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     9481 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     9541 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     9601 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     9661 gcgggggggg ggggnnggsg cgccrggsgg gggggggggg ggsggggggg ggggsggggs
     9721 grggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
     9781 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc
     9841 tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct
     9901 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
     9961 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga
    10021 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt
    10081 gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg
    10141 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc
    10201 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg
    10261 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc
    10321 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg
    10381 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc
    10441 tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg gcggctgtcg
    10501 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact
    10561 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag
    10621 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg
    10681 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga
    10741 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
    10801 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
    10861 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcctcgagg aattcactcc
    10921 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa
    10981 ataccactga gatctgcatg a
//
