LOCUS       sequence-384599-        5508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BLLF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BLLF1(gp350)
FEATURES             Location/Qualifiers
     source          1..5508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3387..3486)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3503..3522)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3504..3522)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3527..3543)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3527..3543)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3974..4765
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4858..5446
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5347..5366
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gaggcagcct tgcttgtgtg tcagtacacc atccagagcc tgatccatct
      721 cacgggtgaa gatcctggtt ttttcaatgt tgagattccg gaattcccat tttaccccac
      781 atgcaatgtt tgcacggcag atgtcaatgt aactatcaat ttcgatgtcg ggggcaaaaa
      841 gcatcaactt gatcttgact ttggccagct gacaccccat acgaaggctg tctaccaacc
      901 tcgaggtgca tttggtggct cagaaaatgc caccaatctc tttctactgg agctccttgg
      961 tgcaggagaa ttggctctaa ctatgcggtc taagaagctt ccaattaacg tcaccaccgg
     1021 agaggagcaa caagtaagcc tggaatctgt agatgtctac tttcaagatg tgtttggaac
     1081 catgtggtgc caccatgcag aaatgcaaaa ccccgtgtac ctgataccag aaacagtgcc
     1141 atacataaag tgggataact gtaattctac caatataacg gcagtagtga gggcacaggg
     1201 gctggatgtc acgctaccct taagtttgcc aacgtcagct caagactcga atttcagcgt
     1261 aaaaacagaa atgctcggta atgagataga tattgagtgt attatggagg atggcgaaat
     1321 ttcacaagtt ctgcccggag acaacaaatt taacatcacc tgcagtggat acgagagcca
     1381 tgttcccagc ggcggaattc tcacatcaac gagtcccgtg gccaccccaa tacctggtac
     1441 agggtatgca tacagcctgc gtctgacacc acgtccagtg tcacgatttc ttggcaataa
     1501 cagtatcctg tacgtgtttt actctgggaa tggaccgaag gcgagcgggg gagattactg
     1561 cattcagtcc aacattgtgt tctctgatga gattccagct tcacaggaca tgccgacaaa
     1621 caccacagac atcacatatg tgggtgacaa tgctacctat tcagtgccaa tggtcacttc
     1681 tgaggacgca aactcgccaa atgttacagt gactgccttt tgggcctggc caaacaacac
     1741 tgaaactgac tttaagtgca aatggactct cacctcgggg acaccttcgg gttgtgaaaa
     1801 tatttctggt gcatttgcga gcaatcggac atttgacatt actgtctcgg gtcttggcac
     1861 ggcccccaag acactcatta tcacacgaac ggctaccaat gccaccacaa caacccacaa
     1921 ggttatattc tccaaggcac ccgagagcac caccacctcc cctaccttga atacaactgg
     1981 atttgctgat cccaatacaa cgacaggtct acccagctct actcacgtgc ctaccaacct
     2041 caccgcacct gcaagcacag gccccactgt atccaccgcg gatgtcacca gcccaacacc
     2101 agccggcaca acgtcaggcg catcaccggt gacaccaagt ccatctccat gggacaacgg
     2161 cacagaaagt aaggcccccg acatgaccag ctccacctca ccagtgacta ccccaacccc
     2221 aaatgccacc agccccaccc cagcagwgac waccccaacc ccaaawgcca ccagccccac
     2281 cccagcagyg actaccccaa ccccaaangc caccagcccc acctynggaa aaacaagycc
     2341 tacctcagca gtgactaccc caaccccaaa tgccaccagc cccacctygg gaaaaacaag
     2401 ccccacctca gcagtgacta ccccaacccc aaatgccacc agccccacct tgggaaaaac
     2461 aagccccacc tcagcagtga ctaccccaac cccaaatgcc accggcccta ctgtgggaga
     2521 aacaagtcca caggcaaatg ccaccaacca caccttagga ggaacaagtc ccaccccagt
     2581 agttaccagc caaccaaaaa atgcaaccag tgctgttacc acaggccaac ataacataac
     2641 ttcaagttca acctcttcca tgtcactgag acccagttca aacccagaga cactcagccc
     2701 ctccaccagt gacaattcaa cgtcacatat gcctttacta acctccgctc acccaacagg
     2761 tggtgaaaat ataacacagg tgacaccagc ctctatcagc acacatcatg tgtccaccag
     2821 ttcgccagca ccccgcccag gcaccaccag ccaagcgtca ggccctggaa acagttccac
     2881 atccacaaaa ccgggggagg ttaatgtcac caaaggcacg cccccccaaa atgcaacgtc
     2941 gccccaggcc cccagtggcc aaaagacggc ggttcccacg gtcacctcaa caggtggaaa
     3001 ggccaattct accaccggtg gaaagcacac cacaggacat ggagcccgga caagtacaga
     3061 gcccaccaca gattacggcg gtgattcaac tacgccaaga ccgagataca atgcgaccac
     3121 ctatctacct cccagcactt ctagcaaact gcggccccgc tggactttta cgagcccacc
     3181 ggttaccaca gcccaagcca ccgtgccagt cccgccaacg tcccagccca gattctcaaa
     3241 cctctccatg ctagtactgc agtgggcctc tctggctgtg ctgacccttc tgctgctgct
     3301 ggtcatggcg gactgcgcct ttaggcgtaa cttgtctaca tcccatacct acaccacccc
     3361 accatatgat gacgccgaga cctatgtacc aactttcttg tacaaagttg gcattataag
     3421 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     3481 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     3541 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     3601 atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc tgctacccaa
     3661 ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc
     3721 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
     3781 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     3841 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     3901 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     3961 aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     4021 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     4081 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     4141 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     4201 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     4261 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     4321 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     4381 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     4441 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     4501 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     4561 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     4621 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     4681 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     4741 gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat cccttaacgt
     4801 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4861 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4921 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4981 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     5041 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5101 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5161 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5221 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5281 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5341 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5401 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5461 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
