LOCUS       sequence-384601-        2171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Toolkit part 2 encoding the PGK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Weinberg ZY, Hilburger CE, Kim M, Cao L, Khalid M, Elmes S, Diwanji 
            D, Hernandez E, Lopez J, Schaefer K, Smith AM, Zhou F, QCRG 
            Structural Biology Consortium, Kumar GR, Ott M, Baker D, El-Samad H
  TITLE     Sentinel cells enable genetic detection of SARS-CoV-2 Spike protein
  JOURNAL   bioRxiv 2021.04.20.440678
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.20.440678"
COMMENT     Sequence Label: PGK Promoter (MTK 2) (pAN2822)
FEATURES             Location/Qualifiers
     source          1..2171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        50..549
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(85..108)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     482..501
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     terminator      complement(572..606)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(677..1336)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1251..1270
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1518..2106
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2007..2026
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg tctcaaacgg ggtaggggag
       61 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
      121 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
      181 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
      241 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcatg tctcactagt
      301 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
      361 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
      421 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
      481 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
      541 ggcctttcgg ccactatgtg agaccagacc aataaaaaac gcccggcggc aaccgagcgt
      601 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
      661 cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
      721 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
      781 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
      841 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
      901 aaacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
      961 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     1021 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     1081 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca
     1141 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     1201 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     1261 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     1321 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     1381 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca
     1441 atcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1501 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1561 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1621 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1681 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1741 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1801 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1861 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1921 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1981 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2041 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2101 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2161 cacatgttct t
//
