LOCUS       sequence-384604-        5409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BSLF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BSLF1
FEATURES             Location/Qualifiers
     source          1..5409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3288..3387)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3404..3423)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3405..3423)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3428..3444)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3428..3444)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3875..4666
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4759..5347
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5248..5267
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg tccgcccccg tcgtcatcaa ggcccttgtg gcctcaaaca ctgacattgc
      721 ggaggccatc ctcgatgcca tcctctcgcg gcctgacgag ggcttccgcc tgttttgttt
      781 gtgccacaac gcctcgcccc tccaccacgt ggcgggttct ctcgtggaac tgcagcttca
      841 cctgcccaag aagcggctga cctcacagag ccgctgcggc ctggttctga cgctgcacct
      901 gccggcggag gaggccttcc ccttcctgcg cggcctcacg ccgctcaccg cggaccgtct
      961 atccacctac ctagatcgcg cgggggccct gcgctcgctc acgcccctcg tggaactgct
     1021 cacactgagc gctaagaaac agccacaggg ggatgccaga ggccgcgtgg cgtggctgcg
     1081 tcccaagatc gtgggctgtc tgcgccgcat ctaccgcgtt aacatctcgg ccaggtggtt
     1141 cattagcacc tttggctccc acgaggccca gttcgtgcta gtcacggctg cttactattt
     1201 ttggggcatt ccttgtacga ttgagaccct ggcgcacctc acggagctat ttacttccga
     1261 gtctggtcag agcctggccg ccgtcacgtc cctggccgag ctgggggagg tctttggctc
     1321 ctcggcatgg gcggagcaga ccgaggcttt tgcccacttt gcacacgaga aactgcggcg
     1381 ggactcgcga gagatccgcg ctgtggcaag gacgatagac gcctacaggg ggcgccttcc
     1441 cctggcctcg gccgatctcg tgcgctacgt ctatttggcc cacgcacagt gcttcaacga
     1501 gggaaccttt aagcgatact ctcagttaac gagcatggga gaaattgggt gtctgccttc
     1561 ggggggcgtc gtgctcccct cgctcctgga caggggcttt gcggagcaca tgcgcaccta
     1621 cttcacccgg gagacttacc tggctgagca tgtgcgtgtc cagcagctga aaatccgcat
     1681 ggagcccccg gccccataca cgtgggaccc cgaccccgat gacggactca tgcgggcctg
     1741 ggccgggctc agtgtggacg tggcccggga gctggtggag ctcgcgcgct ggcacgcgga
     1801 tgagggtccc acataccccc ccacgcttca agggtttcta tgcttggccg gccaggccac
     1861 ctgccggggc cagtggaatc ccaaggaaca attcctccca cccaccgtcc ttcgaagggt
     1921 gcagcggcta ccggtcttcc tctgccattt tgcagacagg cactactttg taatgacagc
     1981 cgctgacccc ttctcatccc acctggcgga ggtcgtctcc accccgacca actgccgcct
     2041 cccagacacg tgtctcacca gggccctctc ctacacccca gtgtactatt cacagaacag
     2101 cctgagcgag cagctctttg tctcccggca tgaatacttt aatccccggc ttccggtctg
     2161 caacctggtc ttggacctgg atctaaagat caagggggcc ccctggtcgc tggaggaaat
     2221 ctatgacctg tgccggaccg tgcggcgtga ggtactgcgc ctcatgcgcc gcctgggtcc
     2281 agtgtccagg gcccacccag tctatttttt caaatcagct tgtccacccg ccgacccgga
     2341 taatatggaa gatgtgctcc ccttttgcat atgcacggga aaactgggct ttcgcgtcat
     2401 caccccccta cctagaggcc atgctattgt gggaacaagc gcagtacaag ggtttgtgtc
     2461 tgtgctgcag aagctcatgg gcctaacggc ctgcctgcgc cgcatgcgtc acaagatcaa
     2521 agagattggg gccccgcttt ttgacagcgg cgtgtatcac gccgggcggt gcatccggct
     2581 gccgcatacc tacaaggtgg acaggggcgg tggtcttagc cggcagctgc gcctctttgt
     2641 ctgtcatccg gaagaggaag acaagcacag ctatgttaag aatgccctca acattcaaaa
     2701 cctcttacat cactcactgc acgtgggctg gccggccccc aaaaccttct gctaccacat
     2761 cgcggatgat gggcgtgact atctaatcca gaggacccgc gagaccctgc cccccaccgt
     2821 ggagaatgtc tgcgccatga tagagggaca tctgggcctg gatctcgtcg cctgggtcag
     2881 ctcctgcatc tggccctcgc tcatgagcac cctggcaaca gctgtgccag aagacaaatt
     2941 cccccagttt ctccatgtca cgtttgagca aaccgggcca aacttagttc aggtgtgcca
     3001 tgcccggggc aggaactttg cgtgcctgag gcatacccac agggccagct ccaagaatgt
     3061 gagggtgttt ctggtactct actacacatc acaggccatc acggtcacct tcatgagtca
     3121 gtgcttcgcc ggtcgctgtg gggccaatca accgaccgcc catttctcca tcagcgtgcc
     3181 cgcctccaga atcataaata gggctgaggc cagtcaagac agcactacat cccagctagc
     3241 ccgtcgtaga gacagacaag atggttcctt ctcagagact ctcccgaacc caactttctt
     3301 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     3361 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     3421 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     3481 cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc
     3541 ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
     3601 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
     3661 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     3721 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     3781 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     3841 gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt
     3901 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     3961 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     4021 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     4081 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     4141 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     4201 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     4261 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     4321 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     4381 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     4441 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     4501 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     4561 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     4621 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc
     4681 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     4741 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4801 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4861 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     4921 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4981 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5041 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5101 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     5161 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     5221 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     5281 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5341 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     5401 tcacatgtt
//
