LOCUS       sequence-384606-        1818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Toolkit part 3b encoding the transmembrane domain of 
            PDGFR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Weinberg ZY, Hilburger CE, Kim M, Cao L, Khalid M, Elmes S, Diwanji 
            D, Hernandez E, Lopez J, Schaefer K, Smith AM, Zhou F, QCRG 
            Structural Biology Consortium, Kumar GR, Ott M, Baker D, El-Samad H
  TITLE     Sentinel cells enable genetic detection of SARS-CoV-2 Spike protein
  JOURNAL   bioRxiv 2021.04.20.440678
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.20.440678"
COMMENT     Sequence Label: PDGFR-TMD (MTK 3B) (pZYW025)
FEATURES             Location/Qualifiers
     source          1..1818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     465..484
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             682..828
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     terminator      complement(848..882)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(953..1612)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1527..1546
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1794..1818,1..564)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
       61 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
      121 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      181 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      241 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      301 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      361 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
      421 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
      481 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
      541 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
      601 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
      661 cgtagtcggt ctcattctaa tgctgtgggc caggacacgc aggaggtcat cgtggtgcca
      721 cactccttgc cctttaaggt ggtggtgatc tcagccatcc tggccctggt ggtgctcacc
      781 atcatctccc ttatcatcct catcatgctt tggcagaaga agccacgtgg atcctgagac
      841 cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
      901 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
      961 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
     1021 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     1081 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     1141 aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata ttctcaataa
     1201 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     1261 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     1321 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     1381 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     1441 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     1501 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     1561 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     1621 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     1681 tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt
     1741 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1801 cctttttttc tgcgcgta
//
