LOCUS       sequence-384609-        1980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Weinberg ZY, Hilburger CE, Kim M, Cao L, Khalid M, Elmes S, Diwanji 
            D, Hernandez E, Lopez J, Schaefer K, Smith AM, Zhou F, QCRG 
            Structural Biology Consortium, Kumar GR, Ott M, Baker D, El-Samad H
  TITLE     Sentinel cells enable genetic detection of SARS-CoV-2 Spike protein
  JOURNAL   bioRxiv 2021.04.20.440678
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.20.440678"
COMMENT     Sequence Label: LCB3_v1.2 (MTK 3a) (pZYW045)
FEATURES             Location/Qualifiers
     source          1..1980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     256..275
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             530..559
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(801..835)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(906..1565)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1480..1499
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1747..1980,1..355)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
       61 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
      121 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
      181 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
      241 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
      301 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
      361 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      421 tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg tctcatatgg ccttgcccgt
      481 tacggcactt ctgctcccac tcgcattgct gcttcacgcc gcccgaccag aacagaaact
      541 cataagtgaa gaagaccttg ggggaggcgg ttccggggga ggcggttcta acgatgacga
      601 gctccatatg cagatgaccg atctggtgta cgaggcacta cactttgcta aagacgagga
      661 aatccagaag cacgtcttcc agctgttcga gaaggccaca aaggcctata aaaataaaga
      721 caggcaaaaa ctggaaaagg ttgtggagga actgaaggaa ttgttagaga gacttctcag
      781 cggttcttga gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
      841 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
      901 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
      961 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     1021 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     1081 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac
     1141 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     1201 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     1261 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     1321 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
     1381 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     1441 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     1501 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     1561 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     1621 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa
     1681 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1741 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1801 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1861 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1921 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
//
