LOCUS       sequence-38461-e        1539 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Calvisi DF, Wang C, Ho C, Ladu S, Lee SA, Mattu S, Destefanis G, 
            Delogu S, Zimmermann A, Ericsson J, Brozzetti S, Staniscia T, Chen 
            X, Dombrowski F, Evert M
  TITLE     Increased lipogenesis, induced by AKT-mTORC1-RPS6 signaling, 
            promotes development of human hepatocellular carcinoma.
  JOURNAL   Gastroenterology. 2011 Mar;140(3):1071-83. Epub 2010 Dec 11.
  PUBMED    21147110
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Gastroenterology."; date: "2011-03"; volume: "140(3)"; pages: 
            "1071-83. Epub 2010 Dec 11"
COMMENT     Sequence Label: pENTR-myr-AKT-HA
FEATURES             Location/Qualifiers
     source          1..1539
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..27
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             1480..1506
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(1511..1527)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1511..1527)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 aagcttatgg ggagcagcaa gagcaagccc aagtctagaa tgaacgacgt agccattgtg
       61 aaggagggct ggctgcacaa acgaggggaa tatattaaaa cctggcggcc acgctacttc
      121 ctcctcaaga acgatggcac ctttattggc tacaaggaac ggcctcagga tgtggatcag
      181 cgagagtccc cactcaacaa cttctcagtg gcacaatgcc agctgatgaa gacagagcgg
      241 ccaaggccca acacctttat catccgctgc ctgcagtgga ccacagtcat tgagcgcacc
      301 ttccatgtgg aaacgcctga ggagcgggaa gaatgggcca ccgccattca gactgtggcc
      361 gatggactca agaggcagga agaagagacg atggacttcc gatcaggctc acccagtgac
      421 aactcagggg ctgaagagat ggaggtgtcc ctggccaagc ccaagcaccg tgtgaccatg
      481 aacgagtttg agtacctgaa actactgggc aagggcacct ttgggaaagt gattctggtg
      541 aaagagaagg ccacaggccg ctactatgcc atgaagatcc tcaagaagga ggtcatcgtc
      601 gccaaggatg aggttgccca cacgcttact gagaaccgtg tcctgcagaa ctctaggcat
      661 cccttcctta cggccctcaa gtactcattc cagacccacg accgcctctg ctttgtcatg
      721 gagtatgcca acgggggcga gctcttcttc cacctgtctc gagagcgcgt gttctccgag
      781 gaccgggccc gcttctatgg tgcggagatt gtgtctgccc tggactactt gcactccgag
      841 aagaacgtgg tgtaccggga cctgaagctg gagaacctca tgctggacaa ggacgggcac
      901 atcaagataa cggacttcgg gctgtgcaag gaggggatca aggatggtgc cactatgaag
      961 acattctgcg gaacgccgga gtacctggcc cctgaggtgc tggaggacaa cgactacggc
     1021 cgtgcagtgg actggtgggg gctgggcgtg gtcatgtatg agatgatgtg tggccgcctg
     1081 cccttctaca accaggacca cgagaagctg ttcgagctga tcctcatgga ggagatccgc
     1141 ttcccgcgca cactcggccc tgaggccaag tccctgctct ccgggctgct caagaaggac
     1201 cctacacaga ggctcggtgg gggctctgag gatgccaagg agatcatgca gcaccggttc
     1261 tttgccaaca tcgtgtggca ggatgtgtat gagaagaagc tgagcccacc tttcaagccc
     1321 caggtcacct ctgagactga caccaggtat ttcgatgagg agttcacagc tcagatgatc
     1381 accatcacgc cgcctgatca agatgacagc atggagtgtg tggacagtga gcggaggccg
     1441 cacttccccc agttctccta ctcagccagt ggcacagcct acccatacga cgtcccagac
     1501 tacgcttgag gatccactag ttctagagcg gcccgataa
//
