LOCUS       sequence-384611-        3279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used
            in designing modular receptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Weinberg ZY, Hilburger CE, Kim M, Cao L, Khalid M, Elmes S, Diwanji 
            D, Hernandez E, Lopez J, Schaefer K, Smith AM, Zhou F, QCRG 
            Structural Biology Consortium, Kumar GR, Ott M, Baker D, El-Samad H
  TITLE     Sentinel cells enable genetic detection of SARS-CoV-2 Spike protein
  JOURNAL   bioRxiv 2021.04.20.440678
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.20.440678"
COMMENT     Sequence Label: SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)
FEATURES             Location/Qualifiers
     source          1..3279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      133..721
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     622..641
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1814..2254
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             2291..2440
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     terminator      complement(2466..2500)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2571..3230)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3145..3164
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat
       61 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
      121 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
      181 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
      241 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
      301 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
      361 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
      421 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
      481 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      541 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      601 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      661 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      721 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      781 gttctttcct gcgttatccc ctgattctgt ggataaccgt agtcggtctc attctatcct
      841 ggactacagc ttcacaggtg gcgctgggcg cgacattccc ccaccgcaga ttgaggaggc
      901 ctgtgagctg cctgagtgcc aggtggatgc aggcaataag gtctgcaacc tgcagtgtaa
      961 taatcacgca tgtggctggg atggtggcga ctgctccctc aacttcaatg acccctggaa
     1021 gaactgcacg cagtctctac agtgctggaa gtattttagc gacggccact gtgacagcca
     1081 gtgcaactcg gccggctgcc tctttgatgg cttcgactgc cagctcaccg agggacagtg
     1141 caaccccctg tatgaccagt actgcaagga ccacttcagt gatggccact gcgaccaggg
     1201 ctgtaacagt gccgaatgtg agtgggatgg cctagactgt gctgagcatg tacccgagcg
     1261 gctggcagcc ggcaccctgg tgctggtggt gctgcttcca cccgaccagc tacggaacaa
     1321 ctccttccac tttctgcggg agctcagcca cgtgctgcac accaacgtgg tcttcaagcg
     1381 tgatgcgcaa ggccagcaga tgatcttccc gtactatggc cacgaggaag agctgcgcaa
     1441 gcacccaatc aagcgctcta cagtgggttg ggccacctct tcactgcttc ctggtaccag
     1501 tggtgggcgc cagcgcaggg agctggaccc catggacatc cgtggctcca ttgtctacct
     1561 ggagatcgac aaccggcaat gtgtgcagtc atcctcgcag tgcttccaga gtgccaccga
     1621 tgtggctgcc ttcctaggtg ctcttgcgtc acttggcagc ctcaatattc cttacaagat
     1681 tgaggccgtg aagagtgagc cggtggagcc tccgctgccc tcgcagctgc acctcatgta
     1741 cgtggcagcg gctgccttcg tgctcctgtt ctttgtgggc tgtggggtgc tgctgtcccg
     1801 caagcgccgg cggatgaagc tgctgagcag catcgagcag gcctgtgaca tctgccggct
     1861 gaagaaactg aagtgcagca aagaaaagcc caagtgcgcc aagtgcctga agaacaactg
     1921 ggagtgccgg tacagcccca agaccaagag aagccccctg accagagccc acctgaccga
     1981 ggtggaaagc cggctggaaa gactggaaca gctgtttctg ctgatcttcc cacgcgagga
     2041 cctggacatg atcctgaaga tggacagcct gcaggacatc aaggccctgc tgaccggcct
     2101 gttcgtgcag gacaacgtga acaaggacgc cgtgaccgac agactggcca gcgtggaaac
     2161 cgacatgccc ctgaccctgc ggcagcacag aatcagcgcc accagcagca gcgaggaaag
     2221 cagcaacaag ggccagcggc agctgacagt gtctgctgct gcaggcggaa gcggaggctc
     2281 tggcggatct gatgccctgg acgacttcga cctggatatg ctgggcagcg acgccctgga
     2341 tgattttgat ctggacatgc tgggatctga cgctctggac gatttcgatc tcgacatgtt
     2401 gggatcagat gcactggatg actttgacct ggacatgctc ggatcaggat cctgagacca
     2461 gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     2521 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
     2581 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     2641 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     2701 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     2761 tcaaaactgg tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac
     2821 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     2881 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     2941 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     3001 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     3061 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     3121 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     3181 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     3241 ttagctcctg aaaatctcga taactcaaaa aatacgccc
//
