LOCUS       sequence-384612-        1956 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Weinberg ZY, Hilburger CE, Kim M, Cao L, Khalid M, Elmes S, Diwanji 
            D, Hernandez E, Lopez J, Schaefer K, Smith AM, Zhou F, QCRG 
            Structural Biology Consortium, Kumar GR, Ott M, Baker D, El-Samad H
  TITLE     Sentinel cells enable genetic detection of SARS-CoV-2 Spike protein
  JOURNAL   bioRxiv 2021.04.20.440678
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.20.440678"
COMMENT     Sequence Label: LCB1_v1.3 (MTK 3a) (pZYW044)
FEATURES             Location/Qualifiers
     source          1..1956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      73..661
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     562..581
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             836..865
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(1083..1117)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1188..1847)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1762..1781
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
       61 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
      121 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
      181 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
      241 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
      301 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
      361 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
      421 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      481 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      541 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      601 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      661 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      721 gttctttcct gcgttatccc ctgattctgt ggataaccgt agtcggtctc atatggcctt
      781 gcccgttacg gcacttctgc tcccactcgc attgctgctt cacgccgccc gaccagaaca
      841 gaaactcata agtgaagaag accttggggg aggcggttcc gggggaggcg gttctgacaa
      901 ggaaaacatt ctccagaaaa tatacgagat catgaaaacc ctcgagcaac tgggccacgc
      961 agaggctagc atgcaggtgt ccgatctgat ctatgaattc atgaagcagg gggacgagag
     1021 actgttggag gaagccgagc ggttacttga agaggtcgaa aggggttctt gagaccagac
     1081 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
     1141 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
     1201 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     1261 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     1321 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     1381 aaactggtga aactcaccca gggattggct gaaacgaaaa acatattctc aataaaccct
     1441 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     1501 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
     1561 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     1621 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     1681 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     1741 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     1801 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     1861 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     1921 tgaaagttgg aacctcttac gtgcccgatc aatcat
//
