LOCUS       sequence-384628-        8078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8078)
  AUTHORS   Chen Z, Zakrzewska S, Hajare HS, Alvarez-Buylla A, Abderemane-Ali F,
            Bogan M, Ramirez D, O'Connell LA, Du Bois J, Minor DL Jr
  TITLE     Definition of a saxitoxin (STX) binding code enables discovery and 
            characterization of the anuran saxiphilin family.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Nov;119(44):e2210114119. doi: 
            10.1073/pnas.2210114119. Epub 2022 Oct 24.
  PUBMED    36279441
REFERENCE   2  (bases 1 to 8078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-11"; volume: "119(44):e2210114119. 
            doi"; pages: " 10.1073/pnas.2210114119. Epub 2022 Oct 24"
COMMENT     Sequence Label: pFastBac1-MaSxph
FEATURES             Location/Qualifiers
     source          1..8078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        156..247
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     172..189
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             2875..2898
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     3564..3585
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3640..3669
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     polyA_signal    3820..3954
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3857..3876)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3911..3930
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3983..4148
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4332..4786
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4419..4438)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4628..4649
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4812..4916
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4917..4985,4986..5777)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5135..5154)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5948..6536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6437..6456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6690..6707
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  6841..7065
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(7132..7665)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7854..7859,7860..7876,7877..7882))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ggaagacctc ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga agtggttcgc
       61 atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc aggactctag ctatagttct
      121 agtggttggc tacgtatact ccggaatatt aatagatcat ggagataatt aaaatgataa
      181 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
      241 tataaatatt ccggattatt cataccgtcc caccatcggg cgcggatccc ggtccgaagc
      301 gcgcggaatt caaaggccta cgtcgacgag ctcactagtc gcggccgcat ggccctgaca
      361 tttcagacgg ccttgtattt tacgattatc ggcctcagct ttgtgacgtc ttctgcacgt
      421 gatgttcgat ggtgtgtcat ttcagattta gagcaaaaga agtgcaatga tcttgtagga
      481 agttgtaaag tgtccgggat tacgctcgtc tgcgtacacc gaagctccac tgagaactgt
      541 atgacagcta tacgcgacga acaggctgat gccatgttcc ttgacagtgg agatgtctat
      601 aaggcttcga tgagtccata caacttaaag ccaataatag ccgaatcata tagttcacac
      661 aaggaatgtc ttaagaaaag acaggaggcg ctaggaggtg ctaacgtctt gggacagttc
      721 gtaccgcaat gtgatgagaa aggttcttac caaccccagc aatgttacgg ttccactggg
      781 cattgctggt gcgtaaacgc aatcggcgaa gaaatcgtcg gtaccaaaac tcaaccaggc
      841 cagacgcaag caacttgtga acgccatgat cagactaagt gcttaaagga ccgtcaaaag
      901 gccctagcaa aaaaaatgat tggtggtttt gtaccacaat gcgacgaaaa ggggaacttt
      961 aaacctcgac aatgccatgg ctctacaggt tactgttggt gtacaaacgc caacggggaa
     1021 aagatcgcag cgacgaatac accaccacaa cagagccccc cgacctgcga acggcacgac
     1081 ctcgactcgt gccactatgc cgtcgcggtt gtcaagaaat cgtcgatctt ccagtttaac
     1141 caactaaaag ggaagcgtag ctgtcacagt ggggtatcaa aaacagatgg ctggaaagcc
     1201 cccgtgagcg tgcttgttga taaaaatcta ctctcttggg acagggctgc caaggaatca
     1261 atagagaaag ctgtgtcaaa attcttttct gactcctgca ttccgggagc caccgaaacc
     1321 aatttatgca accagtgtat gggcgaagga ggcaagaagt gtatgtccag tcatgatgaa
     1381 ccttattacg gagatcacgg ggccttcaga tgcctgaaag aagataaggg tgacgtggca
     1441 ttcttgaaaa atacagtgct gtctgacacc cactccgagg actacgagct tctctgtccc
     1501 gataacacca ggaagcctct gaataaatat aaagagtgca atctcggtaa ggttcccgcg
     1561 gatgctgtag tcactcggaa aagcggcgac aaaattaaag acataaatga cttcctcctt
     1621 gaggcgcaaa agaagcactg tcaattattc tccagcgcgc atggtaagga cttgatgttc
     1681 gacgactcga ccactcaact gtctcttctg ccgcctgaag tcgacgtttt cttctttcta
     1741 ggtgttcagt ggcttaatac gatgaaaacc cttactgcgg atgtgaaacc gccaagtaag
     1801 aataaggtac gctggtgtac cgtgaataac ttggaaaaaa tgaagtgcga cgattggtcg
     1861 gctgtgagcg gaggcgcaat agaatgcacc gaggcatctt caccggagca ttgtattaag
     1921 caaattgtta aaggagaggc agacgcggtt acactggatg ttcagtacat gtatgaggct
     1981 ttaaaatgtg gactactgcc tgcggttgag gagtatcaca ataaggatga tttcgggcct
     2041 tgtaaaacgc ccggttccga gatcaaagac ttcggtgcaa agcgtgccgt cgcattggtc
     2101 aaaaaaagca acaaggatat caactggcat aacctcaagg ggaagaagtc gtgccataca
     2161 ggggtgggcg atcaggcagg ttggattata cctgtcgggt taataagtcg gcagattgat
     2221 aattgcaata ttgattcttt tttttcggag tcatgcgctc cgggttcgga cacgacgtcc
     2281 aatttctgca aattatgcat tggcgaccct tcgaatccca tggctaatac aaaatgcagc
     2341 ctcagcgaca aggaagcata ccatggaaat gagggagcta tacgatgctt ggtagagaag
     2401 ggcgatgtgg ctttcgtgca ccacaccgcc gttttcgaga ataccgacgg caaaaacccc
     2461 gcggaatggg ccgcgaactt aaagagtgag gattttgaaa ttctctgttt agatggttca
     2521 cgggcgcctg tatcgaacta catgacttgc aggttaagcg gagtaccccc aagagcgatt
     2581 gttactcgcg aagaatcagt cagtgacgta gtcagaatcc tgataaacca gcaatctcta
     2641 tttgggagga atggatttga aaaagacatg tttcaaatgt tctcctcgaa taagggccag
     2701 aaccttctat tcaacgatgg gacgcaatgt ttgatagagt ttgaccgcca gccgagagga
     2761 atcatggagg attactttgg gaaaccgtac tataacgcgg tttataacac gtctaagtgt
     2821 gtattgccat cagagttgac ttccgcttgt acatttaagc attgtagcaa ctctctggag
     2881 gtcctgttcc aaggaccaac tgctgccgct gccgtgtcta agggcgaaga gctgttcact
     2941 ggagtggtcc ctatcctggt cgagctggac ggtgacgtga acggccacaa gttctcagtc
     3001 tccggtgaag gcgagggaga cgctacctac ggaaagctga ctctgaagtt catctgcacc
     3061 actggcaagc tgccagtccc ttggcccacc ctggtgacca ctctgactta cggtgtgcag
     3121 tgcttcagcc gctaccctga ccacatgaag cagcacgact tcttcaagtc tgccatgccc
     3181 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggaaacta caagacccgt
     3241 gctgaagtca agttcgaggg cgacactctg gtgaacagga tcgaactgaa gggaatcgac
     3301 ttcaaggagg acggaaacat cctgggtcac aagctggaat acaactacaa ctcacacaac
     3361 gtctacatca tggccgacaa gcagaagaac ggtatcaagg tgaacttcaa gatcagacac
     3421 aacatcgagg acggctccgt ccagctggct gaccactacc agcagaacac tcctatcggt
     3481 gacggacctg tgctgctgcc cgacaaccac tacctgtcca ctcagagcaa gctgtctaag
     3541 gacccaaacg aaaagcgcga ccacatggtc ctgctggagt tcgtgaccgc tgccggcatc
     3601 actctgggaa tggacgaact ctacaaatct ggcggcggac atcatcacca ccaccatcat
     3661 caccaccact aactcgaggc atgcggtacc aagcttgtcg agaagtacta gaggatcata
     3721 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     3781 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     3841 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3901 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgatca
     3961 ctgcttgagc ctaggagatc cgaaccagat aagtgaaatc tagttccaaa ctattttgtc
     4021 atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc
     4081 ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc
     4141 cgcccacagc ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc
     4201 atgtgacaaa ccgtcatctt cggctacttt ttctctgtca cagaatgaaa atttttctgt
     4261 catctcttcg ttattaatgt ttgtaattga ctgaatatca acgcttattt gcagcctgaa
     4321 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4381 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4441 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4501 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4561 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4621 cttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4681 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4741 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     4801 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4861 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4921 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4981 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5041 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5101 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5161 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5221 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5281 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5341 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5401 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5461 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5521 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5581 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5641 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5701 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5761 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5821 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5881 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5941 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6001 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6061 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6121 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6181 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6241 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6301 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6361 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6421 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6481 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6541 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6601 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6661 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     6721 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagaccagc
     6781 cgcgtaacct ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg
     6841 tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt
     6901 cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat
     6961 actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc
     7021 gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa
     7081 tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc
     7141 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag
     7201 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
     7261 gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc
     7321 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag
     7381 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
     7441 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
     7501 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
     7561 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
     7621 agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata
     7681 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
     7741 gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc
     7801 gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac
     7861 gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc
     7921 acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc
     7981 gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca
     8041 aggtgctgtg cacggatctg ccctggcttc aggagatc
//
