LOCUS       sequence-384632-        9763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses only the third computationally called disordered domain of
            mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and 
            a c-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Cherney R, Eberhard Q, Giri G, Mills C, Porrello A, Zhang Z, White 
            D, Trotman JB, Herring L, Dominguez D, Calabrese M
  TITLE     SAFB associates with nascent RNAs and can promote gene expression in
            mouse embryonic stem cells.
  JOURNAL   RNA. 2023 Jul 19:rna.079569.122. doi: 10.1261/rna.079569.122.
  PUBMED    37468167
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2023 
            Jul 19:rna.079569.122. doi: 10.1261/rna.079569.122."
COMMENT     Sequence Label: CAGGS-SAFB-DD3-NLS-3XFLAG-V5
FEATURES             Location/Qualifiers
     source          1..9763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1444..1747
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          2027..3043
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2967..2989
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3051..3070
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4078..4098
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4105..4170
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4189..4230
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    4260..4484
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        4612..4702
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        4750..4961
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4974..5242
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5279..6304
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    6333..6454
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6370..6389)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6424..6443
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7217..7251
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7742..7758)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7742..7758)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7755..7777)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7766..7782
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7790..7796,7797..7814,7815..7820))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7835..7856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7973..7990)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8144..8732)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8224..8243)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8903..9694,9695..9763))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9526..9545
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccagac tagtggatcc attgattatt
     1381 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     1441 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     1501 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     1561 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     1621 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     1681 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     1741 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     1801 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     1861 gggggggggg cgcgcgccrg gsggggsggg gsggggsgrg gggsggggsg gggsgrggcg
     1921 grgaggkgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     1981 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     2041 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     2101 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
     2161 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     2221 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
     2281 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
     2341 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
     2401 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
     2461 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt
     2521 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc
     2581 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc
     2641 cggggrgggc tcgggggrgg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc
     2701 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt
     2761 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
     2821 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc
     2881 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg
     2941 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
     3001 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     3061 ggttattgtg ctgtctcatc attttggcaa acctaggcca ccatgaagcg gtctgttgtg
     3121 tcctttgaca aagttaaaga gtctcgaaag tccagagact cagagtctcg gagggaacgg
     3181 gagcgcagtg agcgggagca gaggctgcag gcccagtggg agcgggagga gcgagagcgg
     3241 ctggagattg ctcgtgagcg cttggcattc caccgccatc ggctagagcg tgaacgcatg
     3301 gagcgggagc ggctggagcg ggagcggatg catgtagaac aggagcggag gcgtgagcaa
     3361 gagcgcattc accgagagcg agaggagctt cggcgtcagc aggagctgcg ctatgaacag
     3421 gagcgccggc ctgctgtgcg caggccctat gaagtggatg gccggcgaga tgatgcctat
     3481 tggccagaag ctaaacgggc tgctctggat gaccgttacc actcagactt cagccgccag
     3541 gaccgctttc acgacttcga ccacagagac agaggccgtt atcccaacca ttctgtggat
     3601 aggagagaag gttcacggtc gatgatggga gacagagaag gacagcatta ccctgaacgc
     3661 catggaggac ctgaacgcca tggccgggac tctcgagatg gctggggcta tggttcgaac
     3721 aagagattga gtgaaggccg ggggctgccc cctcctccca ggcgtgactg gggggagcat
     3781 ggccggcgac ttgaagatga ccgggcatgg cagggcacgg ctgatggagg catgatggag
     3841 cgtgatcata agaggtggca aggtggtgag aggagcatgt ctggccactc ggggcctggt
     3901 cacatgatga accgtggtgg catgtcaggg cgaggcagtt ttgccccagg aggagcttct
     3961 cgtggccatg tgatcccacg tggagggatg caggcaggct ttggaggaca gagccgggga
     4021 agcagaccca gtgatgcccg cttcacccgc cgctatggcc ctggtaccgc ggccgctccc
     4081 aagaagaaga ggaaagtctc gagcgattac aaagaccatg acggtgatta taaagatcat
     4141 gacatcgact acaaggatga cgatgacaag ggctcgggct cgacctcggg taagcctatc
     4201 cctaaccctc tcctcggtct cgattctaca tgaaatctag agtcgacaat gcgtttcgac
     4261 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     4321 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     4381 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     4441 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggaaacac gcggcgacgc
     4501 gttcgaccga ataaaacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt
     4561 ccctgttgat accgggaagc cctgggccaa cttttggcga aaatgagacg ttgatcggca
     4621 cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga
     4681 gttgtcgaga ttttcaggag ctaaagatct ggaagctaag gatctgcgat cgctccggtg
     4741 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     4801 gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     4861 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     4921 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc
     4981 gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc
     5041 gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt
     5101 taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca
     5161 gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt
     5221 ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacaagctgc tagccaccat
     5281 gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     5341 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     5401 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     5461 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     5521 ggagttcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     5581 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
     5641 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     5701 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     5761 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     5821 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     5881 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     5941 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     6001 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     6061 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     6121 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     6181 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     6241 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     6301 atagttttac gtattgagta tttaactaac taaacttgtt tattgcagct tataatggtt
     6361 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6421 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggaattga ctcaaatgat
     6481 gtcaattagt ctatcagaag ctcatctggt ctcccttccg ggggacaaga catccctgtt
     6541 taatatttaa acagcagtgt tcccaaactg ggttcttata tcccttgctc tggtcaacca
     6601 ggttgcaggg tttcctgtcc tcacaggaac gaagtcccta aagaaacagt ggcagccagg
     6661 tttagccccg gaattgactg gattcctttt ttagggccca ttggtatggc tttttccccg
     6721 tatcccccca ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc
     6781 ccgtgccacc ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg
     6841 ggagcgccgg accggagcgg agccccgggc ggctcgctgc tgccccctag cgggggaggg
     6901 acgtaattac atccctgggg gctttggggg ggggctgtcc ctgatatcta taacaagaaa
     6961 atatatatat aataagttat cacgtaagta gaacatgaaa taacaatata attatcgtat
     7021 gagttaaatc ttaaaagtca cgtaaaagat aatcatgcgt cattttgact cacgcggtcg
     7081 ttatagttca aaatcagtga cacttaccgc attgacaagc acgcctcacg ggagctccaa
     7141 gcggcgactg agatgtccta aatgcacagc gacggattcg cgctatttag aaagagagag
     7201 caatatttca agaatgcatg cgtcaatttt acgcagacta tctttctagg gttaatctag
     7261 ctgcatcagg atcatatcgt cgggtctttt ttccggctca gtcatcgccc aagctggcgc
     7321 tatctgggca tcggggagga agaagcccgt gccttttccc gcgaggttga agcggcatgg
     7381 aaagagtttg ccgaggatga ctgctgctgc attgacgttg agcgaaaacg cacgtttacc
     7441 atgatgattc gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc
     7501 acctgggata ccagttcgtc gcggcttttc cggacacagt tccggatggt cagcccgaag
     7561 cgcatcagca acccgaacaa taccggcgac agccggaact gccgtgccgg tgtgcagatt
     7621 aatgacagcg gtgcggcgct gggatattac gtcagcgagg acgggtatcc tggctggatg
     7681 ccgcagaaat ggacatggat accccgtgag ttacccggcg ggcgcgcttg gcgtaatcat
     7741 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7801 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7861 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7921 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7981 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8041 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8101 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8161 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8221 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8281 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8341 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8401 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8461 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8521 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8581 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8641 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8701 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8761 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8821 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8881 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8941 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9001 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9061 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9121 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9181 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9241 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9301 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9361 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9421 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9481 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9541 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9601 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9661 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9721 caaaaaaggg aataagggcg acacggaaat gttgaatact cat
//
