LOCUS       sequence-384633-       11069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse SAFB lacking the first computationally called 
            disordered domain in mammalian cells. Contains a c-terminal 3xFlag 
            tag and a c-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11069)
  AUTHORS   Cherney R, Eberhard Q, Giri G, Mills C, Porrello A, Zhang Z, White 
            D, Trotman JB, Herring L, Dominguez D, Calabrese M
  TITLE     SAFB associates with nascent RNAs and can promote gene expression in
            mouse embryonic stem cells.
  JOURNAL   RNA. 2023 Jul 19:rna.079569.122. doi: 10.1261/rna.079569.122.
  PUBMED    37468167
REFERENCE   2  (bases 1 to 11069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2023 
            Jul 19:rna.079569.122. doi: 10.1261/rna.079569.122."
COMMENT     Sequence Label: CAGGS-safb∆dd1-NLS-3XFLAG-V5
FEATURES             Location/Qualifiers
     source          1..11069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1444..1747
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1749..2026
                     /label=chicken beta-actin promoter
     intron          2027..3043
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2967..2989
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3051..3070
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3116..3125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5384..5404
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5411..5476
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5495..5536
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    5566..5790
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5918..6008
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        6056..6267
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6280..6548
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             6585..7610
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    7639..7760
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7676..7695)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7730..7749
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8523..8557
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9048..9064)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9048..9064)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9061..9083)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9072..9088
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9096..9102,9103..9120,9121..9126))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9141..9162
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9279..9296)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9450..10038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9530..9549)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10209..11000,11001..11069))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10832..10851
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccagac tagtggatcc attgattatt
     1381 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     1441 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     1501 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     1561 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     1621 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     1681 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     1741 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     1801 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     1861 gggggggggg cgcgcgccrg gsggggsggg gsggggsgrg gggsggggsg gggsgrggcg
     1921 gagaggkgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     1981 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     2041 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     2101 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
     2161 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     2221 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
     2281 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
     2341 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
     2401 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
     2461 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt
     2521 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc
     2581 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc
     2641 cggggrgggc tcgggggrgg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc
     2701 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt
     2761 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
     2821 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc
     2881 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg
     2941 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
     3001 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     3061 ggttattgtg ctgtctcatc attttggcaa acctaggatc ggtactgttg gtaaagccac
     3121 catggcggag actctgtcgg gtttaggaga cgcgagtgcg gcgggcgcgg cggccgtgag
     3181 ctccgcggcg tcagagaccg ggacgcggcg gctcagcgac ctgcgggtga tcgacctgcg
     3241 ggcggagctg aagaagcgga acctcgactc gagcggcaac aagagcgtgc tgatggagcg
     3301 gctgaagaag gccattgaag atgaaggtgg gaatcctgat gaaattgaag tcacctcaga
     3361 atgcaacaag aagatgccta agaggcccag caaagggagg aaaccaggag atttagcttt
     3421 ggccagcagc agcaagtctg agtctccaag cactcggtgt cagtggagcg aggcagatgc
     3481 cccgttagca gtagtgaaaa gggagccggc agatgcgcct ggcggaggca ctgggatgga
     3541 ccgcgagcct gtagggctag aggagccagt tgaacagagt agcacggctg cccagctccc
     3601 ggaggccacc agccaggagc tggtgcgagc gcccacggca gccctgagcc ctgagcccca
     3661 agatagcaaa gaagacgtga agaagtttgc ttttgacgct tgtaatgacg tccctgcgcc
     3721 tcctaaagag tcctcagcca gtgagggcgc tgatcagaaa atgagttctg tggaggagga
     3781 ctcggataca aaaaggcttt cccgagaaga gaagggtcgc agcagctgtg gtaggaattt
     3841 ctgggttagt ggcctttcgt ctacaaccag agccacggat ctgaagaacc tgtttagcag
     3901 atatgggaag gtggtgggtg ctaaggttgt gacgaacgcc cggagtcctg gagctcgctg
     3961 ttatggcttt gtcactatgt ccacagctga agaggcaaca aaatgcatta gccatttgca
     4021 caagactgag ctgcatggca agatgatctc agtggaaaag gccaaaagtg agcccactgg
     4081 caaaagagtg cctgacaggc gagatgggga cagcaagaag gagaaggcca gcaccagtga
     4141 cagatctgca aaccttaaga gggaggagaa aggtgagaga aaagacgatg ccaagaagac
     4201 agatgatggg agcacagaga agagcaaaga cgcagatgac cagaaacctg ggccttcaga
     4261 gcgctcgcga acgaccaagt caggaagccg tggcactgag cgcactgtgg tgatggacaa
     4321 gtccaaaggt gtgcctgtca tcagtgtgaa aacatctggg tctaaagaga gagcctccaa
     4381 aagtcaggac cggaagtcag ccagccggga gaagcggtct gttgtgtcct ttgacaaagt
     4441 taaagagtct cgaaagtcca gagactcaga gtctcggagg gaacgggagc gcagtgagcg
     4501 ggagcagagg ctgcaggccc agtgggagcg ggaggagcga gagcggctgg agattgctcg
     4561 tgagcgcttg gcattccacc gccatcggct agagcgtgaa cgcatggagc gggagcggct
     4621 ggagcgggag cggatgcatg tagaacagga gcggaggcgt gagcaagagc gcattcaccg
     4681 agagcgagag gagcttcggc gtcagcagga gctgcgctat gaacaggagc gccggcctgc
     4741 tgtgcgcagg ccctatgaag tggatggccg gcgagatgat gcctattggc cagaagctaa
     4801 acgggctgct ctggatgacc gttaccactc agacttcagc cgccaggacc gctttcacga
     4861 cttcgaccac agagacagag gccgttatcc caaccattct gtggatagga gagaaggttc
     4921 acggtcgatg atgggagaca gagaaggaca gcattaccct gaacgccatg gaggacctga
     4981 acgccatggc cgggactctc gagatggctg gggctatggt tcgaacaaga gattgagtga
     5041 aggccggggg ctgccccctc ctcccaggcg tgactggggg gagcatggcc ggcgacttga
     5101 agatgaccgg gcatggcagg gcacggctga tggaggcatg atggagcgtg atcataagag
     5161 gtggcaaggt ggtgagagga gcatgtctgg ccactcgggg cctggtcaca tgatgaaccg
     5221 tggtggcatg tcagggcgag gcagttttgc cccaggagga gcttctcgtg gccatgtgat
     5281 cccacgtgga gggatgcagg caggctttgg aggacagagc cggggaagca gacccagtga
     5341 tgcccgcttc acccgccgct atggccctgg taccgcggcc gctcccaaga agaagaggaa
     5401 agtctcgagc gattacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
     5461 ggatgacgat gacaagggct cgggctcgac ctcgggtaag cctatcccta accctctcct
     5521 cggtctcgat tctacatgaa atctagagtc gacaatgcgt ttcgactgtg ccttctagtt
     5581 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     5641 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     5701 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     5761 ggcatgctgg ggatgcggtg ggctctatgg aaacacgcgg cgacgcgttc gaccgaataa
     5821 aacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg
     5881 ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca
     5941 actttcacca taatgaaata agatcactac cgggcgtatt ttttgagttg tcgagatttt
     6001 caggagctaa agatctggaa gctaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     6061 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     6121 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     6181 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     6241 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     6301 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     6361 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     6421 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     6481 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     6541 tacagatcca agctgtgacc ggcgcctaca agctgctagc caccatgaaa aagcctgaac
     6601 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga
     6661 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat
     6721 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
     6781 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggag ttcagcgaga
     6841 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa
     6901 ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg
     6961 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta
     7021 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga
     7081 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
     7141 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga
     7201 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc
     7261 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga
     7321 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata
     7381 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg
     7441 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc
     7501 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg
     7561 ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag ttttacgtat
     7621 tgagtattta actaactaaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7681 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7741 aaactcatca atgtatctta tcatgtctgg aattgactca aatgatgtca attagtctat
     7801 cagaagctca tctggtctcc cttccggggg acaagacatc cctgtttaat atttaaacag
     7861 cagtgttccc aaactgggtt cttatatccc ttgctctggt caaccaggtt gcagggtttc
     7921 ctgtcctcac aggaacgaag tccctaaaga aacagtggca gccaggttta gccccggaat
     7981 tgactggatt ccttttttag ggcccattgg tatggctttt tccccgtatc cccccaggtg
     8041 tctgcaggct caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc
     8101 ccgtgcccgg gctgtccccg cacgctgccg gctcggggat gcggggggag cgccggaccg
     8161 gagcggagcc ccgggcggct cgctgctgcc ccctagcggg ggagggacgt aattacatcc
     8221 ctgggggctt tggggggggg ctgtccctga tatctataac aagaaaatat atatataata
     8281 agttatcacg taagtagaac atgaaataac aatataatta tcgtatgagt taaatcttaa
     8341 aagtcacgta aaagataatc atgcgtcatt ttgactcacg cggtcgttat agttcaaaat
     8401 cagtgacact taccgcattg acaagcacgc ctcacgggag ctccaagcgg cgactgagat
     8461 gtcctaaatg cacagcgacg gattcgcgct atttagaaag agagagcaat atttcaagaa
     8521 tgcatgcgtc aattttacgc agactatctt tctagggtta atctagctgc atcaggatca
     8581 tatcgtcggg tcttttttcc ggctcagtca tcgcccaagc tggcgctatc tgggcatcgg
     8641 ggaggaagaa gcccgtgcct tttcccgcga ggttgaagcg gcatggaaag agtttgccga
     8701 ggatgactgc tgctgcattg acgttgagcg aaaacgcacg tttaccatga tgattcggga
     8761 aggtgtggcc atgcacgcct ttaacggtga actgttcgtt caggccacct gggataccag
     8821 ttcgtcgcgg cttttccgga cacagttccg gatggtcagc ccgaagcgca tcagcaaccc
     8881 gaacaatacc ggcgacagcc ggaactgccg tgccggtgtg cagattaatg acagcggtgc
     8941 ggcgctggga tattacgtca gcgaggacgg gtatcctggc tggatgccgc agaaatggac
     9001 atggataccc cgtgagttac ccggcgggcg cgcttggcgt aatcatggtc atagctgttt
     9061 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     9121 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     9181 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     9241 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     9301 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     9361 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     9421 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     9481 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     9541 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9601 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9661 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9721 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9781 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9841 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     9901 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9961 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    10021 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    10081 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    10141 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    10201 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    10261 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10321 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    10381 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10441 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10501 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10561 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10621 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10681 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10741 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10801 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10861 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10921 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10981 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    11041 agggcgacac ggaaatgttg aatactcat
//
