LOCUS       sequence-384638-       12580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination vector to express N-terminal TurboID fusion 
            under UAS promoter, for making transgenic flies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12580)
  TITLE     Drosophila gateway destination vectors to express TurboID fusion 
            proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pWALIUM10-V5-TurboID-GW
FEATURES             Location/Qualifiers
     source          1..12580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1453..2067,2138..2269,2473..2788,2850..3504,
                     3579..3852,4254..4325))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4777..4795
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    4918..4987
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    5225..5655
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    5673..5706
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5719..5813
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    5829..5862
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5875..5969
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        5993..6231
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     6178..6199
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             6261..6302
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6309..7265
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    7305..7429
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7454..7459,7460..7477,7478..7484)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7538..8197
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7604..7623)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8539..8844
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8723..8742
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8885..9009)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          9316..9381
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9511..9531
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9803..9924
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9840..9859)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9894..9913
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    9946..10376
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(10395..10415)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10395..10413)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10434..10450)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10434..10450)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10447..10469)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10458..10474
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10482..10488,10489..10506,10507..10512))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10527..10548
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10665..10682)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10836..11424)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10916..10935)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11595..12386,12387..12455))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12456..12547)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 mayskramky gymmkskwka atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgmcgc tmcmkkrcrm ktymsrtkss ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caagcttcac gacctaggcg cgccctagtt ccagtgaaat ccaagcattt tctaaattaa
      721 atgtattctt attattatag ttgttatttt tgatatatat aaacaacact attatgccca
      781 ccattttttt gagatgcatc tacacaagga acaaacactg gatgtcactt tcagttcaaa
      841 ttgtaacgct aatcactccg aacaggtcac aaaaaattac cttaaaaagt cataatatta
      901 aattagaata aatatagctg tgagggaaat atatacaaat atattggagc aaataaattg
      961 tacatacaaa tatttattac taatttctat tgagacgaaa tgaaccactc ggaaccattt
     1021 gagcgaaccg aatcgcgcgg aactaacgac agtcgctcca aggtcgtcga acaaaaggtg
     1081 aatgtgttgc ggagagcggg tgggagacag cgaaagagca actacgaaac gtggtgtggt
     1141 ggaggtgaat tatgaagagg gcgcgcgatt tgaaaagtat gtatataaaa aatatatccc
     1201 ggtgttttat gtagcgataa acgagttttt gatgtaaggt atgcaggtgt gtaagtcttt
     1261 tggttagaag acaaatccaa agtctacttg tggggatgtt cgaaggggaa atacttgtat
     1321 tctataggtc atatcttgtt tttattggca caaatataat tacattagct ttttgagggg
     1381 gcaataaaca gtaaacacga tggtaataat ggtaaaaaaa aaaaacaagc agttatttcg
     1441 gatatatgtc ggctactcct tgcgtcgggc ccgaagtctt agagccagat atgcgagcac
     1501 ccggaagctc acgatgagaa tggccagacc cacgtagtcc agcggcagat cggcggcgga
     1561 gaagttaagc gtctccagga tgaccttgcc cgaactgggg cacgtggtgt tcgacgatgt
     1621 gcagctaatt tcgcccggct ccacgtccgc ccattggtta atcagcagac cctcgttggc
     1681 gtaacggaac catgagaggt acgacaacca tttgaggtat actggcaccg agcccgagtt
     1741 caagaagaag ccgccaaaga gcaggaatgg tatgataacc ggcggaccca cagacagcgc
     1801 catcgaggtc gaggagctgg cgcaggatat tagatatccg aaggacgttg acacattggc
     1861 caccagagtg accagcgcca ggcagttgaa gaagtgcagc actccggccc gcagtccgat
     1921 catcggatag gcaatcgccg tgaagaccag tggcactgtg agaaaaagcg gcaattcggc
     1981 aatcgttttg cccagaaagt atgtgtcaca gcgataaagt cgacttcggg cctccctcat
     2041 aaaaactggc agctctgagg tgaacaccta aatcgaatcg attcattaga aagttagtaa
     2101 attattgaaa tgcaaatgta ttctaaacat gacttacatt tatcgtggca aagacgtttt
     2161 gaaaggtcat gttggtcagg aagaggaaga tggctccgtt gatattcatc acacccactt
     2221 gcgtgagttg ttggcccaaa aagatgaggc caatcaagat ggcaaccatc tgcaaattaa
     2281 aatgttactc gcatctcatt aatattcgcg agttaaatga aatttattta tcttctgcaa
     2341 aactataaac tatacatctc attgaaaaaa actaagaagg gtgtggaatc aggcaattct
     2401 atctaaaatc tagcgaattt gtttccaaga attgtaagcg ttatatcatt tgtttccact
     2461 ggaaccactc accgttgtct gaataagtcg cacttttacg aggagtggtt ccttgagcac
     2521 cgacagccag gatcgccaca ggaccgcccg gaactgcatg aaccaggtgg ccttgtaggt
     2581 gtacccattc tccggctgct ccagtggctt ctccagattt ttggtggcca acaactgctc
     2641 catatcccgg gctactttgc taatggcaaa attgtcgcat atcttggcga tccgatcacg
     2701 ggactcgatc tcccgtccgg gcacaacggc caacacctgt acgtaaaagt ccgccggatt
     2761 gtagttggta ggacactggg cacccacgct ggataggagt tgagatgtta tgtaatacta
     2821 gataccctta ataaacacat cgaactcact aggaaaagaa gtcgacggct tcgctgggag
     2881 tgcccaagaa agctaccctg ccctcggcca tcagaaggat cttgtcaaag agctcaaaca
     2941 gctcggaaga cggctgatga atggtcagga tgacggtctt gcccttctgc gacagcttct
     3001 tcagcacctg gacgacgctg tgggcggtaa aggagtccag tccggaggtg ggctcatcgc
     3061 agatcagaag cggcggatcg gttagagcct cggaggcgaa tgccagacgc ttcctttctc
     3121 cgccggacag acctttcacc ctgccgggca caccgatgat cgtgtgctga catttgctga
     3181 gcgaaagctc ctggatcacc tgatccacgc gggccactcg ctgccgatag gtcagatgtc
     3241 gtggcatccg caccatggct tggaaaatca ggtgttccct ggccgttagg gagccgataa
     3301 agaggtcatc ctgctggaca taggcgcacc tggcctgcat ctccttggcg tccacaggtt
     3361 ggccattgag cagtcgcatc ccggatggcg atacttggat gccctgcggc gatcgaaagg
     3421 caagggcatt cagcagggtc gtctttccgg caccggaact gcccatcacg gccaaaagtt
     3481 cgcccggata ggccacgccg caaactgagt ttcaaattgg taattggacc ctttattaag
     3541 atttcacaca gatcagccga ctgcgaatag aaactcaccg ttcttgagca aatgtttcct
     3601 gggcgccggt atgtgtcgct cgttgcagaa tagtccgcgt gtccggttga ccagctgccg
     3661 ccatccggag cccggctgat tgaccgcccc aaagatgtcc atattgtgcc aggcataggt
     3721 gaggttctcg gctagttggc cgctccctga accggagtcc tccggcggac tgggtggccg
     3781 gagcgtgccg tagtttttgg cctgcccgaa gccctggtta atgcagctct gcgaagccgc
     3841 tccgctgtca ccctgcaatg ataggggatc tcaaatatca actacaagcg ttatgctcat
     3901 ctaaccccga acaaaaagta ccccgaagta tcctacgaag taggtttata cttttattta
     3961 ttttttgtgc atctaggatc agcttaaaat atctggttgt tatatttttt gtaaaaaaga
     4021 atatagtcga aaatgaatgc ctttagatgt cttgatcatg atatgatctc aaaaattgtc
     4081 ttatatagcg agaacagcta ccagaataat ctgtttcgtg tcactatttg tttgtgcaat
     4141 tgcggtttgg gatttttgtg ggtcgcagtt ctcacgccgc agacaatttg atgttgcaat
     4201 cgcagttcct atagatcaag tgaacttaag atgtatgcac atgtactact cacattgttc
     4261 agatgctcgg cagatgggtg tttgctgcct ccgcgaatta atagctcctg atcctcttgg
     4321 cccattgccg ggatttttca cactttcccc tgcttaccca cccaaaacca atcaccaccc
     4381 caatcactca aaaaacaaac aaaaataaga agcgagagga gttttggcac agcactttgt
     4441 gtttaattga tggcgtaaac cgcttggagc ttcgtcacga aaccgctgac aaaatgcaac
     4501 tgaaggcgga cattgacgct acgtaacgct acaaacggtg gcgaaagaga tagcggacgc
     4561 agcggcgaaa gagacggcga tatttctgtg gacagagaag gaggcaaaca gcgctgactt
     4621 tgagtggaat gtcattttga gtgagaggta atcgaaagaa cctggtacat caaataccct
     4681 tggatcgaag taaatttaaa actgatcaga taagttcaat gatatccagt gcagtaaaaa
     4741 aaaaaaatgt tttttttatc tactttccgc aaaaatgggt tttattaact tacatacata
     4801 ctaggcgcgc cagatcgcaa gaagcttgat atcatcgatc tcgaggctgc atccaacgcg
     4861 ttgggagctc tccggatcaa ttcggcttca ggtaccgtcg acgatgtagg tcacggtctc
     4921 gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccacctc
     4981 acccatctgg tccatcatga tgaacgggtc gaggtggcgg tagttgatcc cggcgaacgc
     5041 gcggcgcacc gggaagccct cgccctcgaa accgctgggc gcggtggtca cggtgagcac
     5101 gggacgtgcg acggcgtcgg ctggtgcgga tacgcggggc agcgtcagcg ggttctcgac
     5161 ggtcacggcg ggcatgtcga caagccgaat tgatccacta gaaggcctaa ttcggtacac
     5221 tagttggcca cgtaataagt gtgcgttgaa tttattcgca aaaacattgc atattttcgg
     5281 caaagtaaaa ttttgttgca taccttatca aaaaataagt gctgcatact ttttagagaa
     5341 accaaataat tttttattgc atacccgttt ttaataaaat acattgcata ccctctttta
     5401 ataaaaaata ttgcatactt tgacgaaaca aattttcgtt gcatacccaa taaaagatta
     5461 ttatattgca tacccgtttt taataaaata cattgcatac cctcttttaa taaagaatat
     5521 tgcatacgtt gacgaaacaa attttcgttg catacccaat aaaagattat tatattgcat
     5581 accttttctt gccataccat ttagccgatc aattgtgctc ggcaacagta tatttgtggt
     5641 gtgccaacca acaacactag tagtaccagc ttataacttc gtataatgta tgctatacga
     5701 agttatctgc aggcaggtcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     5761 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagac
     5821 tcccatggat aacttcgtat aatgtatgct atacgaagtt atggatccgc aggtcggagt
     5881 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
     5941 gtcctccgag cggagtactg tcctccgagc ggagactcgt cgacagcgag cgccggagta
     6001 taaatagagg cgcttcgtct acggagcgac aattcaattc aaacaagcaa agtgaacacg
     6061 tcgctaagcg aaagctaagc aaataaacaa gcgcagctga acaagctaaa caatctgcag
     6121 taaagtgcaa gttaaagtga atcaattaaa agtaaccagc aaccaagtaa atcaactgca
     6181 actactgaaa tctgccaaga agtaattatt gaatacaaga agagaactct gaatagggaa
     6241 ttgggaattc gcacaccatg ggcaagccca tccccaaccc cctgctgggc ctggacagca
     6301 ccgctagcaa agacaatact gtgcctctga agctgatcgc tctcctggct aatggcgagt
     6361 tccatagtgg cgaacagctg ggagaaaccc tgggcatgtc cagggccgct atcaacaagc
     6421 acattcagac tctgcgcgac tggggcgtgg acgtgttcac cgtgcccgga aagggctact
     6481 ctctgcccga gcctatcccg ctgctgaacg ctaaacagat tctgggacag ctggacggcg
     6541 ggagcgtggc agtcctgcct gtggtcgact ccaccaatca gtacctgctg gatcgaatcg
     6601 gcgagctgaa gagtggggat gcttgcattg cagaatatca gcaggcaggg agaggaagca
     6661 gagggaggaa atggttctct ccttttggag ctaacctgta cctgagtatg ttttggcgcc
     6721 tgaagcgggg accagcagca atcggcctgg gcccggtcat cggaattgtc atggcagaag
     6781 cgctgcgaaa gctgggagca gacaaggtgc gagtcaaatg gcccaatgac ctgtatctgc
     6841 aggatagaaa gctggcaggc atcctggtgg agctggccgg aataacaggc gatgctgcac
     6901 agatcgtcat tggtgccggg attaacgtgg ctatgaggcg cgtggaggaa agcgtggtca
     6961 atcagggctg gatcacactg caggaagcag ggattaacct ggacaggaat actctggccg
     7021 ctacgctgat ccgagagctg cgggcagccc tggaactgtt cgagcaggaa ggcctggctc
     7081 catatctgcc acggtgggag aagctggata acttcatcaa tagacccgtg aagctgatca
     7141 ttggggacaa agagattttc gggattagcc gggggattga taaacaggga gccctgctgc
     7201 tggaacagga cggagttatc aaaccctgga tgggcggaga aatcagtctg cggtctgccg
     7261 aaaagagcgg tggcagcggc ggtggtgcgg ccgcggctcg aatcacaagt ttgtacaaaa
     7321 aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
     7381 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt
     7441 aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta
     7501 ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     7561 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     7621 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     7681 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     7741 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     7801 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     7861 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     7921 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
     7981 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     8041 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     8101 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     8161 acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa
     8221 agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga
     8281 tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca
     8341 gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg
     8401 taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa
     8461 atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg
     8521 agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat
     8581 cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc
     8641 cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg
     8701 catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc
     8761 gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt
     8821 aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt
     8881 cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     8941 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta
     9001 caaagtggtg attcgagggt acctctagag caaactagtt ctgatctgct agacaattgt
     9061 tggcatcagg taggcatcac acacgattaa caacccctaa aaatacactt tgaaaatatt
     9121 gaaaatatgt ttttgtatac atttttgata ttttcaaata atacgcagtt ataaaactca
     9181 ttagctaacc cattttttct ttgcttatgc ttacagattg caaagaacta gagccgcggg
     9241 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
     9301 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt
     9361 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg
     9421 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag
     9481 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc
     9541 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact
     9601 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt
     9661 atggaaaaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
     9721 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     9781 aatgaatgga attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     9841 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9901 gtccaaactc atcaatgtat cttatcatgt ctggttccag agctctggcc acgtaataag
     9961 tgtgcgttga atttattcgc aaaaacattg catattttcg gcaaagtaaa attttgttgc
    10021 ataccttatc aaaaaataag tgctgcatac tttttagaga aaccaaataa ttttttattg
    10081 catacccgtt tttaataaaa tacattgcat accctctttt aataaaaaat attgcatact
    10141 ttgacgaaac aaattttcgt tgcataccca ataaaagatt attatattgc atacccgttt
    10201 ttaataaaat acattgcata ccctctttta ataaagaata ttgcatacgt tgacgaaaca
    10261 aattttcgtt gcatacccaa taaaagatta ttatattgca taccttttct tgccatacca
    10321 tttagccgat caattgtgct cggcaacagt atatttgtgg tgtgccaacc aacaacgagc
    10381 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
    10441 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    10501 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
    10561 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
    10621 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
    10681 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    10741 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    10801 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    10861 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    10921 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    10981 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    11041 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    11101 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    11161 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    11221 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    11281 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    11341 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    11401 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    11461 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    11521 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    11581 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    11641 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    11701 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    11761 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    11821 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    11881 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    11941 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    12001 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    12061 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    12121 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    12181 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    12241 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    12301 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    12361 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    12421 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    12481 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    12541 aaacaaatag gggyycmrcr swyrykymsc ckraawgkss
//
