LOCUS       sequence-384655-        5358 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BALF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BALF4
FEATURES             Location/Qualifiers
     source          1..5358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3237..3336)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3353..3372)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3354..3372)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3377..3393)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3377..3393)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3824..4615
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4708..5296
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5197..5216
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg actcggcgta gggtactaag cgtggtcgtg ctgctagccg ccctggcgtg
      721 ccgtctcggt gcgcagaccc cagagcagcc cgcacccccc gccaccacgg tgcagcctac
      781 cgccacgcgt cagcaaacca gctttccttt ccgagtctgc gagctctcca gccacggcga
      841 cctgttccgc ttctcctcgg acatccagtg tccctcgttt ggcacgcggg agaatcacac
      901 ggagggcctg ttgatggtgt ttaaagacaa cattattccc tactcgttta aggtccgctc
      961 ctacaccaag atagtgacca acattctcat ctacaatggc tggtacgcgg actccgtgac
     1021 caaccggcac gaggagaagt tctccgttga cagctacgaa actgaccaga tggataccat
     1081 ctaccagtgc tacaacgcgg tcaagatgac aaaagatggg ctgacgcgcg tgtatgtaga
     1141 ccgcgacgga gttaacatca ccgtcaacct aaagcccacc gggggcctgg ccaacggggt
     1201 gcgccgctac gccagccaga cggagctcta tgacgccccc gggtggttga tatggactta
     1261 cagaacaaga actaccgtca actgcctgat aactgacatg atggccaagt ccaacagccc
     1321 cttcgacttc tttgtgacca ccaccgggca gactgtggaa atgtcccctt tctatgacgg
     1381 gaaaaataag gaaaccttcc atgagcgggc agactccttc cacgtgagaa ctaactacaa
     1441 gatagtggac tacgacaacc gagggacgaa cccgcaaggc gaacgccgag ccttcctgga
     1501 caagggcact tacacgctat cttggaagct cgagaacagg acagcctact gcccgcttca
     1561 acactggcaa acctttgact cgaccatcgc cacagaaaca gggaagtcaa tacattttgt
     1621 gactgacgag ggcacctcta gcttcgtgac caacacaacc gtgggcatag agctcccgga
     1681 cgccttcaag tgcatcgaag agcaggtgaa caagaccatg catgagaagt acgaggccgt
     1741 ccaggatcgt tacacgaagg gccaggaagc cattacatat tttataacga gcggaggatt
     1801 gttattagct tggctacctc tgaccccgcg ctcgttggcc accgtcaaga acctgacgga
     1861 gcttaccact ccgacttcct caccccccag cagtccatcg cccccagccc catccgcggc
     1921 ccgcgggagc acccccgccg ccgttctgag gcgtcggagg cgggatgcgg ggaacgccac
     1981 cacaccggtg ccccccacgg cccccgggaa gtccctgggc accctcaaca atcccgccac
     2041 cgtccagatc caatttgcct acgactccct gcgccgccag atcaaccgca tgctgggaga
     2101 ccttgcgcgg gcctggtgcc tggagcagaa gaggcagaac atggtgctga gagaactaac
     2161 caagattaat ccaaccaccg tcatgtccag catctacggt aaggcggtgg cggccaagcg
     2221 cctgggggat gtcatctcag tctcccagtg cgtgcccgtt aaccaggcca ccgtcaccct
     2281 gcgcaagagc atgagggtcc ctggctccga gaccatgtgc tactcgcgcc ccctggtgtc
     2341 cttcagcttt atcaacgaca ccaagaccta cgagggacag ctgggcaccg acaacgagat
     2401 cttcctcaca aaaaagatga cggaggtgtg ccaggcgacc agccagtact acttccagtc
     2461 cggcaacgag atccacgtct acaacgacta ccaccacttt aaaaccatcg agctggacgg
     2521 cattgccacc ctgcagacct tcatctcact aaacacctcc ctcatcgaga acattgactt
     2581 tgcctccctg gagctgtact cacgggacga acagcgtgcc tccaacgtct ttgacctgga
     2641 gggcatcttc cgggagtaca acttccaggc gcaaaacatc gccggcctgc ggaaggattt
     2701 ggacaatgca gtgtcaaacg gaagaaatca attcgtggac ggcctggggg aacttatgga
     2761 cagtctgggt agcgtgggtc agtccatcac caacctagtc agcacggtgg ggggtttgtt
     2821 tagcagcctg gtctctggtt tcatctcctt cttcaaaaac cccttcggcg gcatgctcat
     2881 tctggtcctg gtggcggggg tggtgatcct ggttatttcc ctcacgaggc gcacgcgcca
     2941 gatgtcgcag cagccggtgc agatgctcta ccccgggatc gacgagctcg ctcagcaaca
     3001 tgcctctggt gagggtccag gcattaatcc cattagtaag acagaattac aagccatcat
     3061 gttagcgctg catgagcaaa accaggagca aaagagagca gctcagaggg cggccggacc
     3121 ctcagtggcc agcagagcat tgcaggcagc cagggaccgt tttccaggcc tacgcagaag
     3181 acgctatcac gatccagaga ccgccgccgc actgcttggg gaggcagaga ctgagtttcc
     3241 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     3301 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     3361 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     3421 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
     3481 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
     3541 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     3601 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     3661 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     3721 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     3781 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
     3841 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     3901 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     3961 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     4021 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     4081 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     4141 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     4201 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     4261 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     4321 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     4381 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     4441 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     4501 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     4561 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc
     4621 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     4681 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4741 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4801 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4861 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4921 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4981 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5041 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5101 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5161 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5221 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5281 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5341 gccttttgct cacatgtt
//
