LOCUS       sequence-384657-        5265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BORF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 5265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BORF2
FEATURES             Location/Qualifiers
     source          1..5265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3144..3243)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3260..3279)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3261..3279)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3284..3300)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3284..3300)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3731..4522
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4615..5203
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5104..5123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gcaacgacca gtcatgtcga gcatgagctc ctctccaaat tgattgatga
      721 gttaaaggtc aaggccaact cagaccccga ggctgatgtc ctggccgggc gcctgctcca
      781 ccgccttaag gccgagtcag ttacacacac agtagccgaa tatctggagg tcttctctga
      841 caaattctac gatgaggaat tcttccagat gcaccgggat gagctggaga cccgagtctc
      901 tgctttcgcg cagagcccgg cctacgagcg catcgtctcc agcggctacc tgtcggccct
      961 gcgctactat gacacctatc tgtatgtggg gcgcagcggg aagcaggaga gtgtgcagca
     1021 cttttacatg cggttagccg gcttctgtgc ctcaaccacc tgcctctacg cgggtctcag
     1081 ggcagccctg cagcgggcca ggccggagat tgagagtgac atggaggtgt ttgattacta
     1141 ctttgagcac ctaacctccc agacggtgtg ctgctccacg ccctttatgc gctttgccgg
     1201 ggtggaaaac tccactctgg ccagctgcat cctcaccacc cccgacctca gctccgagtg
     1261 ggacgtgacc caggccctct ataggcacct ggggcgctac ctctttcagc gagccggggt
     1321 gggtgtaggg gtgacggggg ctggccagga tgggaaacac atcagcctcc tgatgaggat
     1381 gatcaacagc cacgtggagt accacaacta tggctgcaag aggccggtca gtgtggcggc
     1441 ctacatggag ccctggcaca gccagatttt caagtttttg gaaacgaagc tgccggagaa
     1501 ccacgagagg tgcccgggca tctttacggg gctctttgtc cccgagctct tcttcaagct
     1561 ttttagggac acgccctggt cggactggta cctgtttgac cccaaggacg ccggggacct
     1621 ggagaggctc tacggggagg agtttgagcg cgagtactat cggctggtga cagcgggcaa
     1681 gttttgtggg cgggtctcca tcaagtccct gatgttctct atcgtcaact gcgccgtcaa
     1741 ggccggcagc cccttcatcc ttttgaagga ggcctgcaac gcccactttt ggcgcgacct
     1801 gcagggcgag gccatgaacg ccgccaacct gtgcgccgag gtgctgcagc cctcgaggaa
     1861 gtctgtggcc acctgcaatc tggccaacat ctgcctcccg cgctgcctgg tgaatgcgcc
     1921 tctggcggtg cgggcacagc gggccgacac gcagggggat gaactcctgc tggccctccc
     1981 tcgactctca gtcaccctac ctggagaggg ggcagtcggt gatggattct cgctagcccg
     2041 cctcagagat gccacccagt gtgccacctt tgtggtggcc tgctccattc ttcagggatc
     2101 ccccacttat gattccaggg atatggcctc catgggcctc ggggtgcagg gcctggccga
     2161 tgtctttgcg gacctgggct ggcagtacac tgaccctccc tctcgctcgt taaacaagga
     2221 aatattcgaa catatgtact ttacggccct ctgcaccagt agtctgattg gacttcacac
     2281 caggaagatt tttccgggtt tcaaacagag caagtatgcc ggggggtggt ttcactggca
     2341 cgattgggca ggaacagacc tttctattcc cagggaaatt tggtctcgcc tctctgaacg
     2401 cattgtgagg gatgggcttt tcaattcaca gtttatcgcc ctgatgccca cctcaggctg
     2461 tgcccaggtg acgggctgtt cggacgcctt ctaccccttc tatgccaatg cgtccaccaa
     2521 ggtcaccaac aaggaggagg cccttaggcc aaaccggtct ttttggcgtc atgtgcgtct
     2581 ggatgacagg gaagctttga atcttgtcgg gggccgtgtc tcctgcctcc cggaggctct
     2641 gcggcagcgc tacctgcgtt tccaaacggc ctttgattac aaccaggagg acctgattca
     2701 gatgtcccgg gacagggccc cctttgtgga ccagagccaa tctcacagcc tgtttttgcg
     2761 tgaggaagat gccgcgcggg ccagcacgct agccaaccta ctggtgcgca gctacgagct
     2821 gggcctgaag actatcatgt actattgtcg cattgagaag gccgccgatc tgggggtgat
     2881 ggagtgtaag gccagcgcgg ctctgtcggt gccgcgggag gaacagaatg agcggagtcc
     2941 cgctgagcag atgccgcctc gtcccatgga accggcgcag gttgcggggc cggttgacat
     3001 catgagcaag ggcccagggg agggaccagg tgggtggtgt gtgcccgggg gattggaagt
     3061 gtgctataag taccgtcagc tcttctcaga ggatgatctg ttggagactg acggttttac
     3121 tgaacgagcc tgtgaatctt gccaaccaac tttcttgtac aaagttggca ttataagaaa
     3181 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     3241 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     3301 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     3361 atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt
     3421 tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg
     3481 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     3541 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     3601 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     3661 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     3721 gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     3781 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     3841 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     3901 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     3961 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     4021 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     4081 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     4141 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     4201 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     4261 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     4321 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     4381 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     4441 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     4501 caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag
     4561 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4621 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4681 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4741 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     4801 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4861 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4921 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4981 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5041 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5101 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5161 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5221 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
