LOCUS       sequence-384658-       11171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A wildtype adenine transversion base editor (AYBEv0.1, A-to-C and/or
            A-to-T base editor) driven by EF1a promoter, guide RNAs compatible 
            with spCas9 driven by hU6, mCherry driven by CBh promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11171)
  AUTHORS   Tong H, Wang X, Liu Y, Liu N, Li Y, Luo J, Ma Q, Wu D, Li J, Xu C, 
            Yang H
  TITLE     Programmable A-to-Y base editing by fusing an adenine base editor 
            with an N-methylpurine DNA glycosylase.
  JOURNAL   Nat Biotechnol. 2023 Jan 9. doi: 10.1038/s41587-022-01595-6.
  PUBMED    36624150
REFERENCE   2  (bases 1 to 11171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 9. doi: 10.1038/s41587-022-01595-6."
COMMENT     Sequence Label: AYBEv0.1
FEATURES             Location/Qualifiers
     source          1..11171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        268..343
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        430..1608
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          661..1599
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1556..1576
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1625..1644
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1625..1643
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1693..1713
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2308..2331,2332..2334,2335..6408)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2308..6408
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6451..6471
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7447..7467
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7479..7613
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7516..7535)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7570..7589
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        7627..7912
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          8192..8419
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      8438..8447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8444..9154
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8589..8607)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    9177..9401
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(9409..9997)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9489..9508)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10168..10959,10960..11028))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEMGAS
                     LIKHW"
     primer_bind     10791..10810
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11029..11133)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg ggtcttcgag aagacctgtt ttagagctag aaatagcaag ttaaaataag
      301 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg ttttagagct
      361 agaaatagca agttaaaata aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac
      421 aaatggctcg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
      481 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
      541 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
      601 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
      661 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
      721 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
      781 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
      841 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
      901 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
      961 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
     1021 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
     1081 ggcctgcgag cgcggcaacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
     1141 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
     1201 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
     1261 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     1321 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     1381 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     1441 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     1501 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     1561 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg aattagcttg
     1621 gtactaatac gactcactat agggagagcc gccaccatga aacggacagc cgacggaagc
     1681 gagttcgagt caccaaagaa gaagcggaaa gtctctgagg tggagttttc ccacgagtac
     1741 tggatgagac atgccctgac cctggccaag agggcacgcg atgagaggga ggtgcctgtg
     1801 ggagccgtgc tggtgctgaa caatagagtg atcggcgagg gctggaacag agccatcggc
     1861 ctgcacgacc caacagccca tgccgaaatt atggccctga gacagggcgg cctggtcatg
     1921 cagaactaca gactgattga cgccaccctg tacgtgacat tcgagccttg cgtgatgtgc
     1981 gccggcgcca tgatccactc taggatcggc cgcgtggtgt ttggcgtgag gaactcaaaa
     2041 agaggcgccg caggctccct gatgaacgtg ctgaactacc ccggcatgaa tcaccgcgtc
     2101 gaaattaccg agggaatcct ggcagatgaa tgtgccgccc tgctgtgcga tttctatcgg
     2161 atgcctagac aggtgttcaa tgctcagaag aaggcccaga gctccatcaa ctccggagga
     2221 tctagcggag gctcctctgg ctctgagaca cctggcacaa gcgagagcgc aacacctgaa
     2281 agcagcgggg gcagcagcgg ggggtcagac aagaagtaca gcatcggcct ggccatcggc
     2341 accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc
     2401 aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg
     2461 ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac
     2521 accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag
     2581 gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag
     2641 cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac
     2701 cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg
     2761 ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct gatcgagggc
     2821 gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt gcagacctac
     2881 aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa ggccatcctg
     2941 tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct gcccggcgag
     3001 aagaagaatg gcctgttcgg aaacctgatt gccctgagcc tgggcctgac ccccaacttc
     3061 aagagcaact tcgacctggc cgaggatgcc aaactgcagc tgagcaagga cacctacgac
     3121 gacgacctgg acaacctgct ggcccagatc ggcgaccagt acgccgacct gtttctggcc
     3181 gccaagaacc tgtccgacgc catcctgctg agcgacatcc tgagagtgaa caccgagatc
     3241 accaaggccc ccctgagcgc ctctatgatc aagagatacg acgagcacca ccaggacctg
     3301 accctgctga aagctctcgt gcggcagcag ctgcctgaga agtacaaaga gattttcttc
     3361 gaccagagca agaacggcta cgccggctac attgacggcg gagccagcca ggaagagttc
     3421 tacaagttca tcaagcccat cctggaaaag atggacggca ccgaggaact gctcgtgaag
     3481 ctgaacagag aggacctgct gcggaagcag cggaccttcg acaacggcag catcccccac
     3541 cagatccacc tgggagagct gcacgccatt ctgcggcggc aggaagattt ttacccattc
     3601 ctgaaggaca accgggaaaa gatcgagaag atcctgacct tccgcatccc ctactacgtg
     3661 ggccctctgg ccaggggaaa cagcagattc gcctggatga ccagaaagag cgaggaaacc
     3721 atcaccccct ggaacttcga ggaagtggtg gacaagggcg cttccgccca gagcttcatc
     3781 gagcggatga ccaacttcga taagaacctg cccaacgaga aggtgctgcc caagcacagc
     3841 ctgctgtacg agtacttcac cgtgtataac gagctgacca aagtgaaata cgtgaccgag
     3901 ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa aggccatcgt ggacctgctg
     3961 ttcaagacca accggaaagt gaccgtgaag cagctgaaag aggactactt caagaaaatc
     4021 gagtgcttcg actccgtgga aatctccggc gtggaagatc ggttcaacgc ctccctgggc
     4081 acataccacg atctgctgaa aattatcaag gacaaggact tcctggacaa tgaggaaaac
     4141 gaggacattc tggaagatat cgtgctgacc ctgacactgt ttgaggacag agagatgatc
     4201 gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag
     4261 cggcggagat acaccggctg gggcaggctg agccggaagc tgatcaacgg catccgggac
     4321 aagcagtccg gcaagacaat cctggatttc ctgaagtccg acggcttcgc caacagaaac
     4381 ttcatgcagc tgatccacga cgacagcctg acctttaaag aggacatcca gaaagcccag
     4441 gtgtccggcc agggcgatag cctgcacgag cacattgcca atctggccgg cagccccgcc
     4501 attaagaagg gcatcctgca gacagtgaag gtggtggacg agctcgtgaa agtgatgggc
     4561 cggcacaagc ccgagaacat cgtgatcgaa atggccagag agaaccagac cacccagaag
     4621 ggacagaaga acagccgcga gagaatgaag cggatcgaag agggcatcaa agagctgggc
     4681 agccagatcc tgaaagaaca ccccgtggaa aacacccagc tgcagaacga gaagctgtac
     4741 ctgtactacc tgcagaatgg gcgggatatg tacgtggacc aggaactgga catcaaccgg
     4801 ctgtccgact acgatgtgga ccatatcgtg cctcagagct ttctgaagga cgactccatc
     4861 gacaacaagg tgctgaccag aagcgacaag aaccggggca agagcgacaa cgtgccctcc
     4921 gaagaggtcg tgaagaagat gaagaactac tggcggcagc tgctgaacgc caagctgatt
     4981 acccagagaa agttcgacaa tctgaccaag gccgagagag gcggcctgag cgaactggat
     5041 aaggccggct tcatcaagag acagctggtg gaaacccggc agatcacaaa gcacgtggca
     5101 cagatcctgg actcccggat gaacactaag tacgacgaga atgacaagct gatccgggaa
     5161 gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt tccggaagga tttccagttt
     5221 tacaaagtgc gcgagatcaa caactaccac cacgcccacg acgcctacct gaacgccgtc
     5281 gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt gtacggcgac
     5341 tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg agcaggaaat cggcaaggct
     5401 accgccaagt acttcttcta cagcaacatc atgaactttt tcaagaccga gattaccctg
     5461 gccaacggcg agatccggaa gcggcctctg atcgagacaa acggcgaaac cggggagatc
     5521 gtgtgggata agggccggga ttttgccacc gtgcggaaag tgctgagcat gccccaagtg
     5581 aatatcgtga aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc
     5641 aagaggaaca gcgataagct gatcgccaga aagaaggact gggaccctaa gaagtacggc
     5701 ggcttcgaca gccccaccgt ggcctattct gtgctggtgg tggccaaagt ggaaaagggc
     5761 aagtccaaga aactgaagag tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc
     5821 agcttcgaga agaatcccat cgactttctg gaagccaagg gctacaaaga agtgaaaaag
     5881 gacctgatca tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg ccggaagaga
     5941 atgctggcct ctgccggcga actgcagaag ggaaacgaac tggccctgcc ctccaaatat
     6001 gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc cgaggataat
     6061 gagcagaaac agctgtttgt ggaacagcac aagcactacc tggacgagat catcgagcag
     6121 atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa agtgctgtcc
     6181 gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat catccacctg
     6241 tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac caccatcgac
     6301 cggaagaggt acaccagcac caaagaggtg ctggacgcca ccctgatcca ccagagcatc
     6361 accggcctgt acgagacacg gatcgacctg tctcagctgg gaggtgactc tggcggctca
     6421 aaaagaaccg ccgacggcag cgaattcgag cccaagaaga agaggaaagt cctgggaggt
     6481 gacagcggcg ggagcggcgg gagcgggggg agcgtcaccc ccgctttgca gatgaagaaa
     6541 ccaaagcagt tttgccgacg gatggggcaa aagaagcagc gaccagctag agcagggcag
     6601 ccacacagct cgtccgacgc agcccaggca cctgcagagc agccacacag ctcgtccgat
     6661 gcagcccagg caccttgccc cagggagcgc tgcttgggac cgcccaccac tccgggccca
     6721 taccgcagca tctatttctc aagcccaaag ggccacctta cccgactggg gttggagttc
     6781 ttcgaccagc cggcagtccc cctggcccgg gcatttctgg gacaggtcct agtccggcga
     6841 cttcctaatg gcacagaact ccgaggccgc atcgtggaga ccgaggcata cctggggcca
     6901 gaggatgaag ccgcccactc aaggggtggc cggcagaccc cccgcaaccg aggcatgttc
     6961 atgaagccgg ggaccctgta cgtgtacatc atttacggca tgtacttctg catgaacatc
     7021 tccagccagg gggacggggc ttgcgtcttg ctgcgagcac tggagcccct ggaaggtctg
     7081 gagaccatgc gtcagcttcg cagcaccctc cggaaaggca ccgccagccg tgtcctcaag
     7141 gaccgcgagc tctgcagtgg cccctccaag ctgtgccagg ccctggccat caacaagagc
     7201 tttgaccaga gggacctggc acaggatgaa gctgtatggc tggagcgtgg tcccctggag
     7261 cccagtgagc cggctgtagt ggcagcagcc cgggtgggcg tcggccatgc aggggagtgg
     7321 gcccggaaac ccctccgctt ctatgtccgg ggcagcccct gggtcagtgt ggtcgacaga
     7381 gtggctgagc aggacacaca ggcctctggc ggctcaaaaa gaaccgccga cggcagcgaa
     7441 ttcgagccca agaagaagag gaaagtctaa acgcgtcgaa cttgtttatt gcagcttata
     7501 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7561 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcggcggcc
     7621 ggtacccgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     7681 cccattgacg tcaatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     7741 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     7801 tattgacgtc aatgacggta aatggcccgc ctggcattgt gcccagtaca tgaccttatg
     7861 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt
     7921 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
     7981 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggcgcgc
     8041 gccrggsggg gsggggsggg gsggggggsg gggsggggsg rggsggrgrg gkgcggcggc
     8101 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg
     8161 gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt
     8221 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
     8281 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagctga gcaagaggta
     8341 agggtttaag ggatggttgg ttggtggggt attaatgttt aattacctgg agcacctgcc
     8401 tgaaatcact ttttttcagg ttggcagatc cgctagcgcc accatggtga gcaagggcga
     8461 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     8521 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     8581 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     8641 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     8701 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgatga
     8761 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     8821 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaaaaccat
     8881 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     8941 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     9001 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     9061 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     9121 ctccaccggc ggcatggacg agctgtacaa gtaaaccggt ctcttggccc ggatccctgt
     9181 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     9241 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     9301 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     9361 aaacaatagc aggcatgctg gggatgcggt gggctctatg gtggaagctt tccataggct
     9421 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9481 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9541 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     9601 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9661 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9721 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9781 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9841 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9901 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9961 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10021 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    10081 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    10141 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacccatctc
    10201 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    10261 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcggc ttccacgctc
    10321 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    10381 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    10441 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    10501 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    10561 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    10621 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10681 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10741 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    10801 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10861 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10921 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10981 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    11041 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    11101 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    11161 cgtctaagta g
//
