LOCUS       sequence-384660-        5214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BBLF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BBLF4
FEATURES             Location/Qualifiers
     source          1..5214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3093..3192)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3209..3228)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3210..3228)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3233..3249)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3233..3249)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3680..4471
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4564..5152
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5053..5072
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gccgaggagc cgagggcgcc agaggcgctc tcgtccacgt tcatgttgaa
      721 catgacctca gacgcctccg tgaggcgtat agtcaggagg atagggaccc tggctaggcg
      781 ccgcgtccaa caactcccgg atatggagac gttctccccc gagtttgacc cggagctctc
      841 ggagcccccc ttcctgccct tttcagccta tgtaattacg ggaacggcgg gggctggcaa
      901 gagcaccagc gttagctgcc tccaccacac gatggactgc ctggtcacgg gagccacaac
      961 cgtggccgca cagaacctct cccagacact ccgagcctac tgcccaaccg tctatagcgc
     1021 ctttgggttc aagagccgcc acataaatat gacgcagcgg gtcagcagtc atggtcgctc
     1081 tacggacgcc gccctagagg aactccagcg gcgggacctg gccaaatact ggccggtact
     1141 ctccgacatt gccgccgagt tcaggcgaac caagcccagg gggctctact cgggagtctc
     1201 tggccccgcc tttgaggtcc tgagagacat gcaccagggg cagctatgga ccaccaatgt
     1261 gatcgtggtg gacgaagctg gaacgctttc cgtgcacatt ctaacagccg tggtcttttg
     1321 ctactggttc ttcaacgcct ggctgcgtac cccactctac cgccggggcc gcattccctg
     1381 catcgtctgc gtgggctctc ccacccagac agacgccttt cagtcctcct ttagccacga
     1441 gacccaggta aacaagattc gcgagtgcga caacatcctc accttcctgg tgggcaaccc
     1501 cagggccgcg acctacgtgg acgtggcccg caactgggcc ctcttcatca acaacaagcg
     1561 ctgcaccgac gtccagtttg gacacctcat gaagaccctc gagtacggcc tcgagctctc
     1621 gccggacatc ctggcctacg tcgaccgctt cgtcgtcccc agggcggcca taatggaccc
     1681 cgcgcagtac gtgggctgga cccggctctt cctctcccac gccgaggtca agaccttcct
     1741 caccaccctc catgccacgc tcaagactgc cgggcagggg cgtgccgcgc ggggaaccgg
     1801 gggagatggg ggcggggtga ccatgtttac ctgcccggtg gagtgcgagg tcttcctcga
     1861 tcccctggcc cagtacaaga ccctggtcgg gcttccgggc ctcacggccc acacctggct
     1921 ccaaaagaat tatgcacgcc tcggtaacta ctcgcagttc gccgaccaag acatggtccc
     1981 ggtgggcacc gagcaggacg aagaacgggt caaggtcacc tacaatgtca cctacgtcaa
     2041 gcacagctcg gtgtcggtca actgcaagac caaaaagtcc atctgtggct ataccggcac
     2101 cttcggggac tttatggaca cgctcgaggc ggacagcttc gtggaggccc acgggcatga
     2161 gcagcccgag tatgtctaca gcttcctggc ccgactcatc tacggaggca tctacgcttt
     2221 tagccacgga ggacattctc tctgtgaaaa cggggaatac gtggcggagc ttggtgctgt
     2281 gcccctgccc gggcgtacgt gggatccgga ggtgactgct gggatggagc tgggcgaact
     2341 gccgctggag gttgcctggg atggggagcg gagccccgcc gctgtcttct atgcccgagt
     2401 actggctccc ccggccgcaa attctgcccc cctctgctcc ctgttaaaca tatataatga
     2461 cctcagggcc tatttcaggc aatgcctgga tgtggccgtc cgctatggcg gaagggagtt
     2521 cagggacctc cccttctgca catttaccaa caacatgctg attcgcgaca acatagagtt
     2581 cacctcagac gagcctctcc ttcacggcct cctggactac gcctccacca ccgagaacta
     2641 cacgcttctg ggctacacgc atctcaacgt cttctttggc atccgaggaa agcagcagcc
     2701 tcaggacgct ggcagttcac gcatgcccag gctaatggtc aaggatgagg caggcttcgt
     2761 gtgctgcctg gaacacaata ctaacaaact gtatgagacg atagaggaca agtccctgaa
     2821 cctctgcagc atccgtgact atggcattag ctcaaagctg gccatgacca tagccaaggc
     2881 ccagggtctg tccctaaaca aagtcgccat ctgcttcggc agccacagaa acatcaaacc
     2941 cggccatgtg tatgtggcgc tgtcccgggc ccggcactct aattgcgtgg tcatggacag
     3001 gaatccccta tccgagatga tcactgggga ggggaacccc gcgagcggct acatcgtgga
     3061 tgccctaaag aactcacgcg cactactggt ttacccaact ttcttgtaca aagttggcat
     3121 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     3181 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     3241 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     3301 aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct
     3361 acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac
     3421 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     3481 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     3541 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     3601 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     3661 taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
     3721 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     3781 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     3841 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     3901 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     3961 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     4021 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     4081 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     4141 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     4201 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     4261 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     4321 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     4381 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     4441 aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac caaaatccct
     4501 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4561 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4621 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4681 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4741 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4801 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4861 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4921 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4981 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5041 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5101 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5161 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
