LOCUS       sequence-384662-        5037 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BcRF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BcRF1(vTBP)
FEATURES             Location/Qualifiers
     source          1..5037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2916..3015)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3032..3051)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3033..3051)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3056..3072)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3056..3072)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3503..4294
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4387..4975
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4876..4895
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg acacaaggta agagggagat ggggggaggt ctcgagggct tctcttcaca
      721 gctgggtctc ttttacgccc tggcctgcaa ccgcagccca cccacacttc ccgaggatgc
      781 tacccttcta atcaaatggt tggacacggc cctgggcagg gaggccacct tttacgcgtg
      841 tcgggctatg cgtcggcttc tactcggcgt tatccgaatg aatgactgcc aggagctgcc
      901 acccggttta ataattctga gtccgggcac cgtccctggc ccccttggag tccagagtct
      961 ggagcataca gactgcgaaa tatggtcctc tgcccaccct gaccacgctg cccacctccc
     1021 ggtgcccagg gtcatcacat acaccgactg cccgggttcc ataaacacga gctcaatgtt
     1081 tcgccttatc atccgctact tgtctcatca ccaatttgag cgctgcttcg agcagttctg
     1141 ccgcgtggtc ccgcgtcgct tcctagggac ctgtaagcga aactctgcaa agatgctggc
     1201 tcatctgaat caggttacca ggatcccccc ctgtccgccc ttcagcgggc gggaggccag
     1261 actcaagttc cacttcttct cctggagcac attcatgctg tcatggccaa acaatgccac
     1321 actccgggag atcaggacga gggccgccac caacctcacc caccacccac atctagtgga
     1381 tactctgtac cacgcctctc cgcagacccc atttctgaca cgcagcggtg ctctataccg
     1441 cttcgtcacc tgttgcaact gcaccctgcc caatatctcc atccagcagt gcaaggccgg
     1501 ggacagaccg ggggacctgg agatcattct acagagtaac ggcggaggga ggcccgcgag
     1561 cttccagttc ccctcctccc caactggctc cctattgcga tgcatagttg ctgcgtccct
     1621 gctgccggag gtgtccgtgg ggcaccagga gctgtctccg ctgcggtcca gaagccaggg
     1681 agggcagacg gatgtcaggt cgggcccgga cccggcccgg agactggtgg ccctcctgcg
     1741 aagggaagat ggggcaccta aagacccccc tctgggaccg tttggacacc cccgggggcc
     1801 cggcccggcc aagagcgaag acgaggagtc tgagcgtcga gacgcccctc cacccccgct
     1861 cgattccagc ttccaagctt cccggttggt gcccgtgggg cctgggtttc gcctgctcgt
     1921 gttcaacacc aatcgggtga tcaacactaa attggtgtgc tcagagcccc tggtgaagat
     1981 gcgagtttgc aatgtccccc gcctcatcaa caactttgta gcccgcaagt acgtggtgaa
     2041 agagacggcg ttcaccgtca gtctattctt tacggacggg gtgggggcca acctagccat
     2101 caatgtcaat atcagtggca cctatctgag cttcctattg gccatgacgt cactgcggtg
     2161 cttcctgcct gtggaggcta tttatcccgc ggccgtgtca aactggaact cgactctaga
     2221 tctccatggg ctggaaaatc agagcctagt cagagagaac cgaagcgggg tcttttggac
     2281 taccaacttt ccctcggtgg tgtcctgccg ggacggtctc aacgtgtcct ggtttaaggc
     2341 cgcaactgcc accatatctc gagtgcacgg gcagacattg gagcagcacc tgatccgtga
     2401 aatcaccccc atcgtgacgc atcgagaggc aaaaatctcc cggattaaaa accggctctt
     2461 taccctgcta gagctacgca atcggagtca gattcaagtg ctgcacaagc gtttcctgga
     2521 aggcctgcta gactgcgcct ccctcctgcg cctggatccc agctgtatca accgaatcgc
     2581 ctccgagggc ctgtttgatt tctccaagag aagcatcgcc cactccaaaa accgacacga
     2641 gtgcgcgctt ctgggtcaca gacattcggc gaacgtgaca aagctggtgg taaacgagcg
     2701 caagacccgc ctggacatac tgggccgtaa cgctaacttt ttaacgaggt gtaagcatca
     2761 ggttaatcta agacagtcac ctattttcct gaccctcctg aggcacatcc gccgacgtct
     2821 gggcctgggc cgtgcttccg taaaacgaga gattaccctt ctcctggccc acctgcgcaa
     2881 aaagacagcc cccatccact gccgtgatgc tcaagtgcca actttcttgt acaaagttgg
     2941 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     3001 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
     3061 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     3121 taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct
     3181 gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     3241 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     3301 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     3361 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     3421 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     3481 ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact
     3541 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     3601 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     3661 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     3721 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     3781 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     3841 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     3901 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     3961 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     4021 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     4081 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     4141 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     4201 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     4261 gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc
     4321 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4381 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4441 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4501 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     4561 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4621 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4681 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4741 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4801 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4861 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4921 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4981 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
