LOCUS       sequence-384665-        6126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HshnRNPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6126)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-V5-SBP-C1-HshnRNPR_AA
FEATURES             Location/Qualifiers
     source          1..6126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             668..709
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             731..844
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWGGGHVVEGLAGELEQLRARLEHHPQGQREP"
     polyA_signal    2914..3035
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2951..2970)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3005..3024
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3042..3497)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3179..3200)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3391..3410
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3524..3628
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3630..3987
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3630..3821
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3653..3673)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3838..3973
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4022..4816
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4022..4816
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4076..4095)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4686..4705
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5004..5023)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5048..5095
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5424..6012
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5913..5932
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatgggt aagcctatcc ctaaccctct cctcggtctc gattctacgc ccggaagcgg
      721 tagcggtacg atggacgaga agaccaccgg ctgggggggc ggccacgtgg tggagggcct
      781 ggccggcgag ctggagcagc tgcgggccag gctggagcac caccctcagg gccagcggga
      841 gcccagatct cgagggatgg ctaatcaggt gaatggtaat gcggtacagt taaaagaaga
      901 ggaagaacca atggatactt ccagtgtaac tcacacagaa cactacaaga cactgataga
      961 ggcaggcctc ccacagaagg tggcagaaag acttgatgaa atatttcaga caggattggt
     1021 agcttatgtc gatcttgatg aaagagcaat tgatgctctc agggaattta atgaagaagg
     1081 agctctgtct gtactacagc agttcaagga aagtgactta tcacatgttc agaacaaaag
     1141 tgcattttta tgtggagtta tgaagaccta caggcagaga gagaaacagg ggagcaaggt
     1201 gcaagagtcc acaaagggac ctgatgaagc gaagatcaag gccttgcttg agagaactgg
     1261 ttatactctg gatgtaacca caggacagag gaagtatggt ggtcctccac cagacagtgt
     1321 gtactctggc gtgcaacctg gaattggaac ggaggtattt gtaggcaaaa taccaaggga
     1381 tttatatgag gatgagttgg tgcccctttt tgagaaggcc ggacccattt gggatctacg
     1441 tcttatgatg gatccactgt ccggtcagaa tagagggtat gcatttatca ccttctgtgg
     1501 aaaggaagct gcacaggaag ccgtgaaact gtgtgacagc tatgaaattc gccctggtaa
     1561 acaccttgga gtgtgcattt ctgtggcaaa caacagactt tttgttggat ccattccgaa
     1621 gaataagact aaagaaaaca ttttggaaga attcagtaaa gtcacagagg gtttggtgga
     1681 cgttattctc tatcatcaac ccgatgacaa aaagaagaat cgggggttct gcttccttga
     1741 atatgaggat cacaagtcag cagcacaagc cagacgccgg ctgatgagtg gaaaagtaaa
     1801 agtgtgggga aatgtagtta cagttgaatg ggctgaccct gtggaagaac cagatccaga
     1861 agtcatggct aaggtaaaag ttttgtttgt gagaaacttg gctactacgg tgacagaaga
     1921 aatattggaa aagtcatttt ctgaatttgg aaaactcgaa agagtaaaga agttgaaaga
     1981 ttatgcattt gttcattttg aagacagagg agcagctgtt aaggctatgg atgaaatgaa
     2041 tggcaaagaa atagaagggg aagaaattga aatagtctta gccaagccac cagacaagaa
     2101 aaggaaagag cgccaagctg ctagacaggc ctccagaagc actgcgtatg aagattatta
     2161 ctaccaccct cctcctcgca tgccacctcc aattagaggt cggggtcgtg gtggggggag
     2221 aggtggatat ggctaccctc cagattacta cggctatgaa gattactatg atgattacta
     2281 tggttatgat tatcacgact atcgtggagg ctatgaagat ccctactacg gctatgatga
     2341 tggctatgca gtaagaggaa gaggaggagg aaggggaggg cgaggtgctc caccaccacc
     2401 aagggggagg ggagcaccac ctccaagagg tagagctggc tattcacaga ggggggcacc
     2461 tttgggacca ccaagaggct ctaggggtgg cagagggggt cctgctcaac agcagagagg
     2521 ccgtggttcc cgtggatctc ggggcaatcg tgggggcaat gtaggaggca agagaaaggc
     2581 agatgggtac aaccagcctg attccaagcg tcgtcagacc aacaaccaac agaactgggg
     2641 ttcccaaccc atcgctcagc agccgcttca gcaaggtggt gactattctg gtaactatgg
     2701 ttacaataat gacaaccagg aattttatca ggatacttat gggcaacagt ggaagtagct
     2761 gcagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat cataatcagc
     2821 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     2881 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     2941 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3001 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat
     3061 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     3121 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     3181 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     3241 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     3301 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     3361 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     3421 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     3481 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3541 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3601 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     3661 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3721 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     3781 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     3841 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     3901 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     3961 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     4021 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4081 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4141 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     4201 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     4261 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     4321 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     4381 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     4441 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     4501 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     4561 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     4621 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     4681 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     4741 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     4801 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     4861 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     4921 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     4981 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     5041 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     5101 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     5161 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     5221 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     5281 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5341 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5401 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5461 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5521 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5581 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5641 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5701 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5761 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5821 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5881 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5941 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6001 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6061 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6121 atgcat
//
