LOCUS       sequence-384666-        4914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BBLF2/3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BBLF2/3
FEATURES             Location/Qualifiers
     source          1..4914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2793..2892)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2909..2928)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2910..2928)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2933..2949)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2933..2949)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3380..4171
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4264..4852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4753..4772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg atggaaacac ccgcggagag cgtcagggcc cgcgtcagct cggttacctt
      721 ttataatgtt acccagaccg cagggcggtg gtgggcgatt tgggtcgtgg gcatcgtgcc
      781 catcaaaagg gaggacgttg agactctgat cgtggtgcag gcctgccagc cgccgcttgg
      841 aggctccctg gagccccccg tggtcaacgc gccctcgact accgaactca actttttgcg
      901 atgggagcgg gagctcaggc gcagcggggg gctcattgct atgctcgccg atgccgccga
      961 gaaggaccta tttgaccttt catttagaac ccgagaccgc agactcttgt ccgccgccag
     1021 ggtggaggat gagcagggcc tcatcttcca gcctctcttt ccagcacagg tggtctgcca
     1081 aagctgctcg ggggatgatg ggcgggacca acaaccccca cctgttgatg gcttcgggtc
     1141 cgagatggag ggggaacaga catgccccca tgctcagagg cactctgagt ctcccggaca
     1201 gttggacgta tacatcagga caccgcgtgg ggatgttttc acctattcca ccgagactcc
     1261 cgacgacccc tctcccgtcc cctttaggga catcctgcga ccggtcactt atgaagtaga
     1321 tctggtgtcg tccgacgggg ccaccggccg tggtggggac gcccgacggc accgcgtcag
     1381 cctgaaaatt cttgagccgg ctgggggatt cgagtcctgg cttgtcaact cttggagtat
     1441 ggccgggggc gggctctacg ccttcctccg cagtatctat gcctcctgct acgccaacca
     1501 cagaggcacc aagcccatct tctacctcct ggaccccgaa ctctgcccag ggggctcaga
     1561 tttccagccc tatgtcccgg gctttccctt cctgcccatc cattatgtgg gacgggcgag
     1621 gccggccttc tggcaccggg cgccacacag cgagggtctc ctgctactgg acctgaacct
     1681 gggagtctct gggacgcccc tggcagacgc cctcctgggc ctcgacgcgc ggtcagggca
     1741 acgacgcggc tcgctgctcc tacaacaaat ctggcccccg acccgaaagg agattaaccc
     1801 tcgccacgtt tgcacgcggg aaggcggcga gggagggggg gaggacgaga cgacggtggt
     1861 cgggcgcgcg gaggccacag ccatccttga ggctgatgcc acttggtggc tctacgaatt
     1921 ggcccgctgc cacctctctg ccaggggcgc ccccgtggga acgcctgatg gcggagggca
     1981 ggcgcgggac gcgcaaacct ggctgcgggc ccttcaccgc tatggcacct cagacacgcg
     2041 acgggcactc gggggcctct acaccgccgt cacccgggtt ctcctacacg cggccgctga
     2101 cctaggactg acctgggctt atgcagacga gttcatcctg ggctttgtgg caccaacctc
     2161 cgcccatcct tcagaggagc cactagcaca ggctttcctg caaggcgtga aagactcgga
     2221 ggatgccagc cggctcgacc gggatgtgat gggtggagag gccaccgtgg cccgcagaca
     2281 cattcgggtg aaggctcgac gcgggcccgg gtgcctactg atggccatct ttcaggggga
     2341 tctttacgtg ggtggatgta gggagcactc ggggcccttt ttggtttggc acgaagcctt
     2401 ctcctggacc ctggaccaac tagcggcgag acccgaggca gacaaggccc cgccatccca
     2461 cgaccacctg ttgaccctgg tccgcgacct gacccggaga ctggcccccg ggcggcgccg
     2521 aaacaggttt tgggctcttc cgcgagcctg gcttcagcgg ctgcggaggg ctgggctgcg
     2581 cctctctgga agccacgtgt gtctcctgga caaggacgga gcgcgcccgg ccccctgcca
     2641 gacggccact gagcatggtc tcagccccac cgcctacttt cgagagatta tggcctttct
     2701 gcttgatgtg atatcggccc tccaccccgg ctacaccatt cctatggaaa tcacgcgaga
     2761 gacagattta ctgatgactg ttctcagttt attcccaact ttcttgtaca aagttggcat
     2821 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     2881 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     2941 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     3001 aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct
     3061 acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac
     3121 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     3181 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     3241 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     3301 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     3361 taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
     3421 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     3481 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     3541 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     3601 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     3661 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     3721 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     3781 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     3841 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     3901 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     3961 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     4021 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     4081 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     4141 aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac caaaatccct
     4201 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4261 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4321 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4381 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4441 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4501 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4561 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4621 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4681 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4741 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4801 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4861 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
