LOCUS       sequence-384670-        4905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BXLF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BXLF2
FEATURES             Location/Qualifiers
     source          1..4905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2784..2883)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2900..2919)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2901..2919)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2924..2940)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2924..2940)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3371..4162
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4255..4843
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4744..4763
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg cagttgctct gtgttttttg cctggtgttg ctatgggagg tgggggctgc
      721 cagcctcagc gaggttaagc tgcacctgga catagagggg catgcttcgc attacaccat
      781 cccatggacc gaactgatgg caaaggtccc aggccttagc ccagaggcgc tgtggagaga
      841 ggcaaatgtc accgaagatt tggcgtctat gcttaaccgc tacaagttaa tttacaagac
      901 gtctggtacc cttggtattg cgctggccga gcctgtcgat atccctgctg tctctgaagg
      961 atccatgcaa gtggatgcat ctaaggtcca tcccggagtc attagcggcc tgaattcccc
     1021 tgcctgcatg cttagtgccc cccttgagaa gcagctcttc tactatattg gcaccatgct
     1081 gcccaacacg cggccacaca gctatgtctt ttatcagctg cgctgtcact tgtcttatgt
     1141 ggccctgtcc atcaacgggg acaagtttca gtacacgggg gccatgactt ctaaatttct
     1201 gatgggcacc tacaagcgag tgaccgagaa gggagatgag catgtgttga gcctggtctt
     1261 tggcaagacg aaggacctgc cggatctgag ggggcctttt agttacccat ccttaaccag
     1321 tgcccaaagc ggggactatt ccctggtgat tgttacaacc tttgtgcatt atgccaactt
     1381 tcacaactac tttgtaccca acctgaagga tatgttttcc cgagccgtca ccatgacagc
     1441 cgccagctac gctcgctacg ttctccagaa actggtcctg ctggagatga agggaggctg
     1501 ccgggagccg gaactggaca cggaaacgct gactaccatg tttgaggttt ctgtggcctt
     1561 ctttaaggtg ggtcatgctg tgggtgagac tggcaatggc tgcgtggacc tccgctggtt
     1621 ggccaagagc ttctttgagc tgactgtcct gaaagacatc atcggcatat gttatggggc
     1681 cactgtcaag ggcatgcaat cctacgggct ggagcgcttg gccgccatgc tgatggccac
     1741 ggtcaagatg gaggagctgg gtcacctgac tactgagaaa caggagtacg cgctgaggtt
     1801 agccaccgtc ggctacccca aggccggggt ttacagtggc ctcattggag gcgccacatc
     1861 tgtgcttctc tcggcctaca accgccaccc ccttttccag cccctgcata ccgtgatgag
     1921 agagaccctg tttatcggca gccacgtggt gctacgcgag ttgcggctga acgtgactac
     1981 ccaggggccc aaccttgccc tataccaact gctgtccacc gccctgtgct cggccctaga
     2041 gattggggag gttttgcggg ggctagccct ggggacagag agcgggctct tctcaccgtg
     2101 ctacctcagc ctacgatttg acctcacacg agacaagctg ctgagcatgg ccccccagga
     2161 ggcaacgctg gaccaggcgg ccgtttcaaa tgctgtggat gggtttcttg ggcggctctc
     2221 tttggagcga gaagacaggg atgcgtggca tctccccgcc tacaaatgcg tggacaggct
     2281 cgacaaagtt ctgatgatta tcccgctcat caatgtgaca ttcataatct ctagtgaccg
     2341 tgaggtccga ggctcggcgc tatacgaggc cagcaccacc tatctcagca gctctctctt
     2401 tctctccccc gttataatga ataaatgttc gcagggtgct gtggctgggg agccccgcca
     2461 gattccaaag atccagaatt ttaccaggac gcagaaatcc tgcatttttt gtggctttgc
     2521 cctgctcagt tatgatgaaa aggaaggcct ggaaactaca acctacatca cctcccagga
     2581 agtccaaaac tccatcttga gctccaacta ctttgatttt gacaacctcc acgttcacta
     2641 tctgctgctg accaccaacg ggactgtcat ggaaattgcg ggcctgtatg aagaaagagc
     2701 acacgttgtt ttggcaataa tcctgtactt tattgctttt gctctgggta tctttctggt
     2761 tcacaagatt gttatgtttt tccttccaac tttcttgtac aaagttggca ttataagaaa
     2821 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     2881 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     2941 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     3001 atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt
     3061 tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg
     3121 ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     3181 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     3241 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     3301 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     3361 gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     3421 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     3481 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     3541 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     3601 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     3661 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     3721 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     3781 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     3841 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     3901 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     3961 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     4021 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     4081 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     4141 caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag
     4201 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4261 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4321 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4381 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     4441 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4501 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4561 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4621 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4681 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4741 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4801 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4861 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
