LOCUS       sequence-384672-        4857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BGRF1-BDRF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-B-GD-RF1
FEATURES             Location/Qualifiers
     source          1..4857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2736..2835)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2852..2871)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2853..2871)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2876..2892)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2876..2892)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3323..4114
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4207..4795
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4696..4715
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg ctgtatgcct cgcagcgcgg ccgtctgacc gagaacctaa gaaacgccct
      721 tcagcaggac agcaccacgc aaggctgcct gggtgccgag accccgagta ttatgtacac
      781 aggggccaag tcagacaggt gggctcaccc tctggtgggc acaattcacg ccagtaattt
      841 atattgccca atgcttcgag catactgccg ccactatggc cccaggcccg tgtttgtagc
      901 ttctgatgaa tcattaccca tgttcggtgc gagccctgcc cttcacaccc cagtccaggt
      961 ccagatgtgc ctactaccag agctacgcga cacgttacag cgcctgctgc caccacccaa
     1021 tcttgaagac tccgaggcct tgacggaatt caagaccagc gtgtcctctg cccgtgccat
     1081 ccttgaggac cccaactttt tggagatgag agagtttgtc accagcctgg ccagcttcct
     1141 gagtggtcag tacaagcaca agcccgcccg cctagaagca ttccagaaac aagtagtgtt
     1201 acattctttt tattttctga tctcaatcaa atctttagag attacagaca ccatgtttga
     1261 catctttcaa agtgctttcg gattggaaga aatgacgctg gagaagctgc acatttttaa
     1321 gcaaaaagcc agcgtgtttc ttatccccag gcgccacggc aagacctgga tagtcgtggc
     1381 catcatcagc ctcatcctct cgaatctctc caacgtgcaa ataggctacg tggctcacca
     1441 gaaacatgtc gcgtccgccg ttttcactga aattattgac accttgacca agagcttcga
     1501 ctccaagcgt gtagaggtca acaaggagac cagcaccatc acgtttaggc acagtgggaa
     1561 aatctccagc accgtaatgt gtgccacctg cttcaataag aatagcatcc gggggcagac
     1621 atttcacctc ttgtttgtgg acgaggctaa ctttatcaag aaggaggccc tgccggcgat
     1681 cctgggcttt atgcttcaga aggatgccaa gattatcttc atctcgtctg tgaactcggc
     1741 tgaccaggcc accagctttc tttataagct gaaggatgct caggagcggc tgctgaacgt
     1801 ggtaagttat gtgtgtcagg agcatcggca agattttgac atgcaggaca gcatggtctc
     1861 atgcccctgc tttcgcctgc acatcccgtc ctacatcacc atggacagca acatccgagc
     1921 aaccaccaac ctctttctgg acggggcctt tagcaccgag ctgatgggtg acacctcctc
     1981 gctgagccag ggtagcctga gccgcactgt gcgtgacgat gccatcaacc agctggagct
     2041 ctgccgggtt gacaccctca acccccgagt agccggacgc ctagcctcct ccctctacgt
     2101 gtacgttgat ccggcctata ccaacaacac atccgcatca ggcaccggaa tcgccgccgt
     2161 gactcacgac agggcggacc ctaacagggt catcgtcctg ggcctggaac acttcttcct
     2221 caaggaccta acaggggacg ctgccctcca gatcgccacc tgcgtcgtgg ccctcgtctc
     2281 ctcgatcgtc accctgcacc cccacttgga ggaggtgaag gtagccgtgg agggcaacag
     2341 cagtcaggac tctgcggtgg ccattgcctc aatcattggg gaatcctgcc ccctcccctg
     2401 cgccttcgtg cacaccaagg acaagacgtc cagcctgcag tggcccatgt acctcctgac
     2461 taatgagaag tccaaggcct ttgagaggct catctacgca gtgaacacgg ccagcctttc
     2521 tgccagtcag gtcaccgtct ccaacaccat ccagctctcc ttcgatccgg tcctctatct
     2581 catctcccag atcagggcca tcaagcccat ccctctccgc gacggtacct acacctacac
     2641 cggcaagcag cgcaacctct ctgacgacgt gctggttgcg ctagtcatgg ctcattttct
     2701 cgcaacaaca cagaagcaca cgttcaagaa agttcatcca actttcttgt acaaagttgg
     2761 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     2821 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
     2881 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     2941 taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct
     3001 gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     3061 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     3121 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     3181 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     3241 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     3301 ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact
     3361 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     3421 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     3481 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     3541 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     3601 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     3661 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     3721 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     3781 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     3841 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     3901 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     3961 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     4021 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     4081 gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc
     4141 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4201 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4261 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4321 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     4381 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4441 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4501 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4561 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4621 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4681 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4741 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4801 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
