LOCUS       sequence-384677-       11639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse SAFB in mammalian cells. Contains a c-terminal 
            3xFlag tag and a c-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11639)
  AUTHORS   Cherney R, Eberhard Q, Giri G, Mills C, Porrello A, Zhang Z, White 
            D, Trotman JB, Herring L, Dominguez D, Calabrese M
  TITLE     SAFB associates with nascent RNAs and can promote gene expression in
            mouse embryonic stem cells.
  JOURNAL   RNA. 2023 Jul 19:rna.079569.122. doi: 10.1261/rna.079569.122.
  PUBMED    37468167
REFERENCE   2  (bases 1 to 11639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2023 
            Jul 19:rna.079569.122. doi: 10.1261/rna.079569.122."
COMMENT     Sequence Label: CAGGS-SAFB-NLS-3XFLAG-V5
FEATURES             Location/Qualifiers
     source          1..11639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1444..1747
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1749..2026
                     /label=chicken beta-actin promoter
     intron          2027..3043
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2967..2989
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3051..3070
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3116..3125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5954..5974
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5981..6046
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6065..6106
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    6136..6360
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        6488..6578
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        6626..6837
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6850..7118
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             7155..8180
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    8209..8330
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8246..8265)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8300..8319
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   9093..9127
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9618..9634)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9618..9634)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9631..9653)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9642..9658
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9666..9672,9673..9690,9691..9696))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9711..9732
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9849..9866)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10020..10608)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10100..10119)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10779..11570,11571..11639))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11402..11421
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccagac tagtggatcc attgattatt
     1381 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     1441 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     1501 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     1561 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     1621 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     1681 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     1741 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     1801 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     1861 gggggggggg cgcgcgccrg gsggggsggg gsggggsgrg gggsggggsg gggcgaggcg
     1921 grgaggkgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     1981 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     2041 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     2101 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
     2161 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     2221 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
     2281 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
     2341 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
     2401 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
     2461 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt
     2521 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc
     2581 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc
     2641 cggggrgggc tcgggggrgg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc
     2701 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt
     2761 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
     2821 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc
     2881 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg
     2941 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
     3001 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     3061 ggttattgtg ctgtctcatc attttggcaa acctaggatc ggtactgttg gtaaagccac
     3121 catggcggag actctgtcgg gtttaggaga cgcgagtgcg gcgggcgcgg cggccgtgag
     3181 ctccgcggcg tcagagaccg ggacgcggcg gctcagcgac ctgcgggtga tcgacctgcg
     3241 ggcggagctg aagaagcgga acctcgactc gagcggcaac aagagcgtgc tgatggagcg
     3301 gctgaagaag gccattgaag atgaaggtgg gaatcctgat gaaattgaag tcacctcaga
     3361 atgcaacaag aagatgccta agaggcccag caaagggagg aaaccagaag acgagggtgt
     3421 ggaggacaac gggctcgagg agaattctgg ggatgggcag gaggatgtgg agaccagcct
     3481 ggagaacctg caagacatgg atatgatgga catcagtgtg ctggatgagg ctgacattga
     3541 caatggcagc gtggcagact gtgtggagga agaggaggag gccacactcc ctgagggcct
     3601 agcagacagc actgaactgg tagaaggaga tttgaaaggg ctcccagaac agctgcagga
     3661 gcatgctata gatgacaagg acaccgttaa caatgtagat acgtcatcat ctgacttcac
     3721 catgctgcag gagatggaag aggcatcttt ggagccagaa aatgagaaaa tactcgacat
     3781 tttgggggaa acttgtaaat ctgagccagt aaaagaagaa ggttcggagc tggagcagcc
     3841 ctttgcccag gccacgagta gcgtggggcc agacaggaag ctggcggagg aagaggacct
     3901 atttgagagc tgcggccacc cggaagagga agaggaagag gaggaagaag atcaggaaga
     3961 ggagcaggag gaggagggag atttagcttt ggccagcagc agcaagtctg agtctccaag
     4021 cactcggtgt cagtggagcg aggcagatgc cccgttagca gtagtgaaaa gggagccggc
     4081 agatgcgcct ggcggaggca ctgggatgga ccgcgagcct gtagggctag aggagccagt
     4141 tgaacagagt agcacggctg cccagctccc ggaggccacc agccaggagc tggtgcgagc
     4201 gcccacggca gccctgagcc ctgagcccca agatagcaaa gaagacgtga agaagtttgc
     4261 ttttgacgct tgtaatgacg tccctgcgcc tcctaaagag tcctcagcca gtgagggcgc
     4321 tgatcagaaa atgagttctg tggaggagga ctcggataca aaaaggcttt cccgagaaga
     4381 gaagggtcgc agcagctgtg gtaggaattt ctgggttagt ggcctttcgt ctacaaccag
     4441 agccacggat ctgaagaacc tgtttagcag atatgggaag gtggtgggtg ctaaggttgt
     4501 gacgaacgcc cggagtcctg gagctcgctg ttatggcttt gtcactatgt ccacagctga
     4561 agaggcaaca aaatgcatta gccatttgca caagactgag ctgcatggca agatgatctc
     4621 agtggaaaag gccaaaagtg agcccactgg caaaagagtg cctgacaggc gagatgggga
     4681 cagcaagaag gagaaggcca gcaccagtga cagatctgca aaccttaaga gggaggagaa
     4741 aggtgagaga aaagacgatg ccaagaagac agatgatggg agcacagaga agagcaaaga
     4801 cgcagatgac cagaaacctg ggccttcaga gcgctcgcga acgaccaagt caggaagccg
     4861 tggcactgag cgcactgtgg tgatggacaa gtccaaaggt gtgcctgtca tcagtgtgaa
     4921 aacatctggg tctaaagaga gagcctccaa aagtcaggac cggaagtcag ccagccggga
     4981 gaagcggtct gttgtgtcct ttgacaaagt taaagagtct cgaaagtcca gagactcaga
     5041 gtctcggagg gaacgggagc gcagtgagcg ggagcagagg ctgcaggccc agtgggagcg
     5101 ggaggagcga gagcggctgg agattgctcg tgagcgcttg gcattccacc gccatcggct
     5161 agagcgtgaa cgcatggagc gggagcggct ggagcgggag cggatgcatg tagaacagga
     5221 gcggaggcgt gagcaagagc gcattcaccg agagcgagag gagcttcggc gtcagcagga
     5281 gctgcgctat gaacaggagc gccggcctgc tgtgcgcagg ccctatgaag tggatggccg
     5341 gcgagatgat gcctattggc cagaagctaa acgggctgct ctggatgacc gttaccactc
     5401 agacttcagc cgccaggacc gctttcacga cttcgaccac agagacagag gccgttatcc
     5461 caaccattct gtggatagga gagaaggttc acggtcgatg atgggagaca gagaaggaca
     5521 gcattaccct gaacgccatg gaggacctga acgccatggc cgggactctc gagatggctg
     5581 gggctatggt tcgaacaaga gattgagtga aggccggggg ctgccccctc ctcccaggcg
     5641 tgactggggg gagcatggcc ggcgacttga agatgaccgg gcatggcagg gcacggctga
     5701 tggaggcatg atggagcgtg atcataagag gtggcaaggt ggtgagagga gcatgtctgg
     5761 ccactcgggg cctggtcaca tgatgaaccg tggtggcatg tcagggcgag gcagttttgc
     5821 cccaggagga gcttctcgtg gccatgtgat cccacgtgga gggatgcagg caggctttgg
     5881 aggacagagc cggggaagca gacccagtga tgcccgcttc acccgccgct atggccctgg
     5941 taccgcggcc gctcccaaga agaagaggaa agtctcgagc gattacaaag accatgacgg
     6001 tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagggct cgggctcgac
     6061 ctcgggtaag cctatcccta accctctcct cggtctcgat tctacatgaa atctagagtc
     6121 gacaatgcgt ttcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     6181 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     6241 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     6301 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     6361 aaacacgcgg cgacgcgttc gaccgaataa aacctgtgac ggaagatcac ttcgcagaat
     6421 aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt tggcgaaaat
     6481 gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata agatcactac
     6541 cgggcgtatt ttttgagttg tcgagatttt caggagctaa agatctggaa gctaaggatc
     6601 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     6661 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     6721 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     6781 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     6841 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     6901 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     6961 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     7021 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     7081 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctaca
     7141 agctgctagc caccatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
     7201 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
     7261 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
     7321 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
     7381 aagtgcttga cattggggag ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
     7441 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
     7501 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
     7561 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
     7621 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
     7681 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
     7741 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
     7801 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
     7861 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
     7921 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
     7981 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
     8041 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
     8101 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
     8161 gtccgagggc aaaggaatag ttttacgtat tgagtattta actaactaaa cttgtttatt
     8221 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8281 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     8341 aattgactca aatgatgtca attagtctat cagaagctca tctggtctcc cttccggggg
     8401 acaagacatc cctgtttaat atttaaacag cagtgttccc aaactgggtt cttatatccc
     8461 ttgctctggt caaccaggtt gcagggtttc ctgtcctcac aggaacgaag tccctaaaga
     8521 aacagtggca gccaggttta gccccggaat tgactggatt ccttttttag ggcccattgg
     8581 tatggctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt
     8641 tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg
     8701 gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc
     8761 ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg ctgtccctga
     8821 tatctataac aagaaaatat atatataata agttatcacg taagtagaac atgaaataac
     8881 aatataatta tcgtatgagt taaatcttaa aagtcacgta aaagataatc atgcgtcatt
     8941 ttgactcacg cggtcgttat agttcaaaat cagtgacact taccgcattg acaagcacgc
     9001 ctcacgggag ctccaagcgg cgactgagat gtcctaaatg cacagcgacg gattcgcgct
     9061 atttagaaag agagagcaat atttcaagaa tgcatgcgtc aattttacgc agactatctt
     9121 tctagggtta atctagctgc atcaggatca tatcgtcggg tcttttttcc ggctcagtca
     9181 tcgcccaagc tggcgctatc tgggcatcgg ggaggaagaa gcccgtgcct tttcccgcga
     9241 ggttgaagcg gcatggaaag agtttgccga ggatgactgc tgctgcattg acgttgagcg
     9301 aaaacgcacg tttaccatga tgattcggga aggtgtggcc atgcacgcct ttaacggtga
     9361 actgttcgtt caggccacct gggataccag ttcgtcgcgg cttttccgga cacagttccg
     9421 gatggtcagc ccgaagcgca tcagcaaccc gaacaatacc ggcgacagcc ggaactgccg
     9481 tgccggtgtg cagattaatg acagcggtgc ggcgctggga tattacgtca gcgaggacgg
     9541 gtatcctggc tggatgccgc agaaatggac atggataccc cgtgagttac ccggcgggcg
     9601 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     9661 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     9721 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     9781 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     9841 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     9901 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     9961 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    10021 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    10081 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    10141 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    10201 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    10261 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    10321 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    10381 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    10441 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    10501 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    10561 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    10621 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    10681 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    10741 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    10801 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    10861 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    10921 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    10981 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    11041 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    11101 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    11161 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    11221 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    11281 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    11341 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    11401 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    11461 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    11521 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    11581 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcat
//
