LOCUS       sequence-384678-        9719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse hnRNPU with a 3xFLAG tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9719)
  AUTHORS   Cherney R, Eberhard Q, Giri G, Mills C, Porrello A, Zhang Z, White 
            D, Trotman JB, Herring L, Dominguez D, Calabrese M
  TITLE     SAFB associates with nascent RNAs and can promote gene expression in
            mouse embryonic stem cells.
  JOURNAL   RNA. 2023 Jul 19:rna.079569.122. doi: 10.1261/rna.079569.122.
  PUBMED    37468167
REFERENCE   2  (bases 1 to 9719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2023 
            Jul 19:rna.079569.122. doi: 10.1261/rna.079569.122."
COMMENT     Sequence Label: pb-miRE-tre-3xFLAG-hnRNPU
FEATURES             Location/Qualifiers
     source          1..9719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1367..1681
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1375..1393
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1411..1429
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1446..1464
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1482..1500
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1518..1536
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1553..1571
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1589..1607
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1654..1678
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1720..1785
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    4216..4440
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        4568..4658
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        4706..4917
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4930..5198
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5235..6260
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    6289..6410
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6326..6345)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6380..6399
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7173..7207
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7698..7714)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7698..7714)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7711..7733)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7722..7738
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7746..7752,7753..7770,7771..7776))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7791..7812
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7929..7946)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8100..8688)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8180..8199)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8859..9650,9651..9719))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9482..9501
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccagac tagttcgagt ttactcccta
     1381 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgatgtcgag
     1441 tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
     1501 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tatccctatc
     1561 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagg
     1621 taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc
     1681 ctggaccggt cgtttagtga accgtcagaa ttaaccatgg actacaaaga ccatgacggt
     1741 gattataaag atcatgacat cgattacaag gatgacgatg acaagagcgg atccatgagt
     1801 tcttcgcctg ttaatgtcaa gaagctgaag gtgtcggagc tgaaggagga gctcaagaag
     1861 cggcgcctct ccgacaaggg cctcaaggcc gatctcatgg atcgactcca ggccgcgctg
     1921 gacaacgagg caggaggccg ccccgccatg gagcccggga acggcagtct cgacctaggt
     1981 ggcgatgcgg ccgggcgctc gggagcgggc ctagagcagg aggccgcggc tggcgccgaa
     2041 gacgacgagg aggaggaggg catcgccgct ctggacggcg accagatgga gctgggcgag
     2101 gagaacgggg cggcgggggc ggctgacgcc ggcgcgatgg aggaagagga ggcagcgtcg
     2161 gaagacgaga acggcgacga ccagggcttc caggaggggg aagacgagct cggcgacgag
     2221 gaggagggcg cgggcgacga gaacggtcac ggggagcagc agtcccaacc gccggcagcg
     2281 gcggcgcagc agcagccttc ccagcagcgt ggtgccggca aggaggccgc gggcaagagc
     2341 agcggcccca cctcgctctt cgcggtgacg gtggcgccgc caggggcgag gcagggccaa
     2401 cagcaggcgg gaggagacgg caaaacagaa cagaaaggtg gagataaaaa gagaggcgtt
     2461 aaaagaccgc gagaagatca tggccgaggg tattttgagt acatcgaaga aaacaagtac
     2521 agcagagcca agtctcctca gccacctgtt gaagaagaag acgaacactt cgatgacaca
     2581 gtggtttgtc ttgatactta taattgtgat ctgcatttta aaatctcgag agaccgtctg
     2641 agtgcttctt cccttactat ggagagtttt gctttcctgt gggctggagg aagagcttcc
     2701 tacggtgtgt caaaaggcaa agtctgcttt gagatgaagg taacagagaa gattccagta
     2761 agacacttat atacaaaaga tattgatata catgaagttc ggattggctg gtcactaacc
     2821 acaagtggaa tgttgcttgg tgaagaagaa ttttcttacg ggtattctct gaaaggaata
     2881 aaaacatgca actgtgagac agaagattat ggggagaagt ttgatgaaaa tgatgtgatt
     2941 acatgctttg ctaactttga aactgatgaa gttgaactct cttatgcgaa gaatggacaa
     3001 gatcttggtg ttgcctttaa gatcagtaag gaagttcttg ctgaccggcc actattccca
     3061 catgttctct gccataactg tgcagttgaa tttaatttcg gtcaaaagga aaagccatac
     3121 tttccaatac ctgaagactg tacttttatc caaaatgtcc ccttagagga ccgagttaga
     3181 ggaccaaaag gacctgaaga gaagaaggat tgtgaggttg taatgatgat tggcttgcca
     3241 ggagctggaa aaactacctg ggttactaaa catgcagctg aaaaccctgg gaaatacaac
     3301 attcttggaa caaatacgat tatggacaag atgatggtgg caggttttaa gaagcaaatg
     3361 gcagatactg gaaaactgaa cacactgttg cagagagccc cacagtgtct tggcaagttt
     3421 attgaaattg ctgcccgtaa gaagcgaaat tttattctgg atcagacaaa tgtgtctgct
     3481 gctgcccaga gaagaaaaat gtgcctgttt gcaggcttcc agcggaaagc tgttgtagtg
     3541 tgcccaaaag atgaagacta taagcagagg acacagaaga aggcagaagt agaggggaag
     3601 gacctaccag aacatgctgt cctcaagatg aaaggaaact tcacccttcc agaggttgca
     3661 gaatgctttg atgaaataac ctatgttgaa cttcagaaag aggaagccca aaagcttttg
     3721 gagcaatata aagaagaaag caaaaaggca ctgccaccag aaaagaagca aaacactggc
     3781 tcaaagaaaa gcaataagaa taagagtggc aagaaccagt tcaacagagg tggtggccat
     3841 agaggccgtg gaggattcaa tatgcgaggt ggcaatttca gaggaggagc tcctgggaat
     3901 cgtggtggat ataataggag aggcaacatg ccacagagag gtggtggcgg tggaagtggt
     3961 ggaattggct atccataccc acgtggccct gtttttcctg gccgaggtgg ttactcaaac
     4021 agagggaatt acaacagagg tggaatgccc aacagaggga actataacca gaacttcaga
     4081 ggacgaggaa ataatcgtgg ctacaaaaat caatctcagg gctacaatca gtggcagcag
     4141 ggtcaattct ggggtcagaa gccatggagt cagcattatc accaaggata ttattgagtc
     4201 gacaatgcgt ttcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     4261 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     4321 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     4381 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     4441 aaacacgcgg cgacgcgttc gaccgaataa aacctgtgac ggaagatcac ttcgcagaat
     4501 aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt tggcgaaaat
     4561 gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata agatcactac
     4621 cgggcgtatt ttttgagttg tcgagatttt caggagctaa agatctggaa gctaaggatc
     4681 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     4741 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     4801 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     4861 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     4921 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     4981 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     5041 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     5101 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     5161 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctaca
     5221 agctgctagc caccatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
     5281 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
     5341 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
     5401 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
     5461 aagtgcttga cattggggag ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
     5521 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
     5581 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
     5641 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
     5701 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
     5761 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
     5821 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
     5881 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
     5941 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
     6001 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
     6061 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
     6121 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
     6181 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
     6241 gtccgagggc aaaggaatag ttttacgtat tgagtattta actaactaaa cttgtttatt
     6301 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6361 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     6421 aattgactca aatgatgtca attagtctat cagaagctca tctggtctcc cttccggggg
     6481 acaagacatc cctgtttaat atttaaacag cagtgttccc aaactgggtt cttatatccc
     6541 ttgctctggt caaccaggtt gcagggtttc ctgtcctcac aggaacgaag tccctaaaga
     6601 aacagtggca gccaggttta gccccggaat tgactggatt ccttttttag ggcccattgg
     6661 tatggctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt
     6721 tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg
     6781 gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc
     6841 ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg ctgtccctga
     6901 tatctataac aagaaaatat atatataata agttatcacg taagtagaac atgaaataac
     6961 aatataatta tcgtatgagt taaatcttaa aagtcacgta aaagataatc atgcgtcatt
     7021 ttgactcacg cggtcgttat agttcaaaat cagtgacact taccgcattg acaagcacgc
     7081 ctcacgggag ctccaagcgg cgactgagat gtcctaaatg cacagcgacg gattcgcgct
     7141 atttagaaag agagagcaat atttcaagaa tgcatgcgtc aattttacgc agactatctt
     7201 tctagggtta atctagctgc atcaggatca tatcgtcggg tcttttttcc ggctcagtca
     7261 tcgcccaagc tggcgctatc tgggcatcgg ggaggaagaa gcccgtgcct tttcccgcga
     7321 ggttgaagcg gcatggaaag agtttgccga ggatgactgc tgctgcattg acgttgagcg
     7381 aaaacgcacg tttaccatga tgattcggga aggtgtggcc atgcacgcct ttaacggtga
     7441 actgttcgtt caggccacct gggataccag ttcgtcgcgg cttttccgga cacagttccg
     7501 gatggtcagc ccgaagcgca tcagcaaccc gaacaatacc ggcgacagcc ggaactgccg
     7561 tgccggtgtg cagattaatg acagcggtgc ggcgctggga tattacgtca gcgaggacgg
     7621 gtatcctggc tggatgccgc agaaatggac atggataccc cgtgagttac ccggcgggcg
     7681 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7741 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7801 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7861 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7921 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7981 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8041 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8101 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8161 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8221 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8281 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8341 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8401 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8461 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8521 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8581 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8641 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8701 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8761 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8821 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8881 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8941 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     9001 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9061 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9121 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9181 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9241 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9301 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9361 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9421 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9481 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9541 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9601 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9661 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcat
//
