LOCUS       sequence-384680-        4842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BALF3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BALF3
FEATURES             Location/Qualifiers
     source          1..4842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2721..2820)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2837..2856)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2838..2856)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2861..2877)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2861..2877)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3308..4099
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4192..4780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4681..4700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg tcggggctgc tggcggcggc gtacagccag gtgtacgccc tggcggttga
      721 gctgagcgtg tgcacccggc tggacccccg gagtctggac gtggctgcgg tggtgcgcaa
      781 cgccggcctg ctggccgagc tggaggccat cctccttccc cgtttgagac ggcagaatga
      841 ccgtgcatgc agcgccctgt ccctggagct ggtgcacctg ctagagaact cgagagaggc
      901 ctctgccgcg ctgctcgccc ctggtagaaa gggtacccgg gtcccgcctc tccgtacccc
      961 ctcagtcgcg tactctgtgg agttttacgg ggggcataaa gtcgatgtaa gtttgtgcct
     1021 aataaatgac atagagattt taatgaagag aatcaatagc gtgttttatt gcatgtctca
     1081 caccatgggg ctggagagcc tggaacgggc cctggatctg ctgggccgct ttcggggcgt
     1141 aagtcccatc ccagacccgc gcctctacat cacctctgtg ccctgctggc gctgtgtggg
     1201 cgagctgatg gttctgccca accacggcaa cccttccacg gcagagggga cccacgtctc
     1261 ctgtaaccac ctggcggtgc cggtgaatcc ggagccggtc tcgggactgt ttgagaatga
     1321 agtccgccag gcggggctcg ggcacctgtt ggaggctgag gagaaggcga ggccgggcgg
     1381 cccagaggag ggcgcggtcc cgggcccggg gcggccggag gcagaggggg cgaccagagc
     1441 gctggacacc tacaacgtct tctcgacagt gcccccggag gtggcggagc tctcggagct
     1501 cctctattgg aactctggcg gccatgctat cggtgcaacg gggcaggggg agggtggcgg
     1561 ccattcccgc ctctctgccc tgtttgcccg ggagcgtcgc ctggccctgg tgcggggggc
     1621 ctgcgaggag gcgctggcgg gggcaaggct gactcacctg tttgacgccg tggctcccgg
     1681 ggccacggag cggctcttct gcggcggggt ctacagctcc tcgggcgacg cggtggaggc
     1741 gctgaaggcg gactgcgccg ccgccttcac ggcgcacccc cagtaccggg ccatcctgca
     1801 aaagaggaac gagctgtaca cgcggctcaa ccgagccatg cagcggttgg gccgaggcga
     1861 ggaggaggcg tcccgggaga gcccggaggt gccccggccg gctggggcac gagagcccgg
     1921 cccgtccggc gccctctcgg acgcgctcaa gcgcaaggag cagtacctgc gccaggtggc
     1981 caccgagggt ctggccaagc tgcagtcctg cctggcgcaa cagagcgaga ccctgaccga
     2041 gaccctgtgc ctgcgcgtct ggggggacgt ggtctactgg gagctggccc gcatgcgcaa
     2101 ccacttcctc tacagacggg ccttcgtctc gggtccctgg gaggacaggc gcgccggcga
     2161 gggtgccgcc tttgagaatt ccaagtacat caaaacacac ctgtttaccc agaccctgag
     2221 ctcggagcac ctgcacgcgc tgacgcacag cctgtacacc ttcatcacgg ggcccctggc
     2281 ggaggagagc gggctctttc ccccacccag caacgtggcc ctggctcgct gctgtgacgc
     2341 cgcaggtacg ctgccccacc agaaggcatt cctgacttcc ctgatatggc caggcatcga
     2401 gccgagcgac tggatagaga cctccttcaa cagcttctac agcgtacccg ggggctcact
     2461 ggcatctagc cagcaaattc tgtgccgggc gctgcgcgag gccgtcctaa ccgtgtccct
     2521 ctacaacaag acctgggggc ggtccttgat cctgcgccgg gcggacgcgg tcagccccgg
     2581 ccaggccctg cccccggatg ggctttacct cacgtacgac tctgaccgcc ccctaattct
     2641 tctgtataag ggcagggggt gggtatttaa ggatctatat gcccttctct acctgcacct
     2701 ccaaatgaga gatgactcgg cgccaacttt cttgtacaaa gttggcatta taagaaagca
     2761 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     2821 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2881 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2941 atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc
     3001 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
     3061 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     3121 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     3181 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     3241 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     3301 aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     3361 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     3421 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     3481 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     3541 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     3601 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     3661 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     3721 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     3781 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     3841 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     3901 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     3961 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     4021 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     4081 caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta
     4141 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4201 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4261 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4321 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4381 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4441 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4501 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4561 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4621 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4681 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4741 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4801 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
