LOCUS       sequence-384689-        3302 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of GAL4 DNA-binding domain (BD)-FHIP-L fusion protein in 
            yeast (yeast two-hybrid assays).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Mattera R, Williamson CD, Ren X, Bonifacino JS
  TITLE     The FTS-Hook-FHIP (FHF) complex interacts with AP-4 to mediate 
            perinuclear distribution of AP-4 and its cargo ATG9A.
  JOURNAL   Mol Biol Cell. 2020 Apr 15;31(9):963-979. doi: 
            10.1091/mbc.E19-11-0658. Epub 2020 Feb 19.
  PUBMED    32073997
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E19-11-0658"; journalName: "Mol Biol Cell"; date: 
            "2020-04-15- 15"; volume: "31"; issue: "9"; pages: "963-979"
COMMENT     Sequence Label: pGBT9-FHIP-L
FEATURES             Location/Qualifiers
     source          1..3302
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      746..755
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3263..3287)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 tcatcggaag agagtagtaa caaaggtcaa agacagttga ctgtatcgcc ggaattcatg
       61 atgtcatcgg tttcgacaga aagcaaactc cagcaggctg tgagcctaca gggagttgac
      121 ccagaaacat gcatgattgt atttaaaaac cactgggcac aggttgtgaa aatcttggag
      181 aagcacgacc ccttgaagaa cacccaggca aaatatgggt ctatccctcc agatgaggcc
      241 agtgccgtgc agaattacgt agaacacatg ctcttcttgt tgattgaaga gcaagccaaa
      301 gatgctgcaa tggggccgat tctggaattt gtggtctctg agaacatcat ggagaaactt
      361 ttcctttgga gcttgagaag ggagtttact gatgagacta aaattgagca gctaaagatg
      421 tatgagatgt tggtcaccca gtcgcaccag cctctgctgc accacaaacc cattctgaag
      481 cctctgatga tgttgctgag ctcttgttca ggaacaacca cccccactgt ggaggagaag
      541 ctggttgtcc tactcaatca gctctgttcc attcttgcca aagatccatc cattttagaa
      601 ctcttcttcc acactagtga agaccaaggc gctgccaact tcctcatctt ctcccttctg
      661 attcccttca ttcaccgaga ggggtcagta ggccagcaag ctcgggatgc attgctcttc
      721 atcatgtctc tttctgctga gaacaccatg gtggcccatc acatcgtgga gaacacctac
      781 ttttgtccag tacttgcaac tgggctcagt ggtctctact cttccctgcc tacaaagcta
      841 gaagagaaag gcgaggaatg gcactgcctt ctgaaagatg actggcttct acttccttct
      901 cttgtccagt tcatgaactc cctggagttt tgcaatgcag tcatacaggt ggctcacccc
      961 ttgattcgaa atcagcttgt caattacatt tacaatggat ttttggtacc agtcttggct
     1021 cctgctctcc ataaggtgac tgtggaagag gtcatgacca caactgcata tctggacctt
     1081 ttcctgcgta gcatctccga gccagcacta cttgagatct tcctccgttt tatcctattg
     1141 caccagcacg agaatgtcca catcctagac actctcacga gtcgaatcaa caccccgttt
     1201 cggctttgtg tggtgtctct ggcattattc agaactctca ttggtttaca ttgtgaagat
     1261 gtgatgttac agctagttct aaggtatctg atcccctgca atcacatgat gctgagtcag
     1321 aggtgggctg tgaaggagag agactgttac tctgtttctg cggccaagct tctcgccttg
     1381 actcctgtct gctgctccag cgggatcact ctgacgctgg ggaaccaaga gagggattat
     1441 attctctggt caaagtgtat gcatgacact tcagggcctg tggagcggcc attccccgaa
     1501 gcgttctccg agtcagcctg cattgtggag tatgggaaag ccctggacat cagctacctg
     1561 cagtacctgt gggaggccca caccaacatc ctccgctgca tgagggactg ccgtgtctgg
     1621 tccgccctgt atgatggcga ctcccccgac cctgagatgt ttctccagag tctgacggag
     1681 gagggcagtg tgagctcggc ctgccctgtg ttcgggctcc cgcaacaact ccccaggaag
     1741 acaggacctc agctggctcc cagaaaggac aagagccaga cagagctgga atgggatgac
     1801 agctatgaca ctggaatctc ctcaggggct gacgtgggct ccccagggcc ttatgatgat
     1861 ctggaggttt caggcccccc agcacccatt gatcccccca aacacatcca ggagatgaag
     1921 aagaatgccc tcctgctctt caaagggtcc tacatagaag agtcggactt tcaggatgat
     1981 gtgatggtgt acaggctgtg tgctgagaag gactccgagg acatgaagga ttctcaggag
     2041 gaagctgcta ggccaccagc tgaagcccag gctgaagttc agagtgtccc catcaacaac
     2101 ggccccctcc tcagcaccca gccagagaca gattcagagg aggagtggaa tagggacaat
     2161 tcagacccgt ttcacagtga gcccaaggag ccaaagcaag agagggaacc tgaagcagcc
     2221 ccagaatcca actcagagtt agcatcccct gcccctgagg cagagcacag ctctaacctg
     2281 acagccgccc acccggagag cgaggagctc attgcccagt atgaccaaat cattaaagag
     2341 ctggattccg gcgccgaggg cttgatggaa cagaattacc ccacacctga tcccttgctt
     2401 ctcactaagg aggaagaagg gaaggaagag agtaaaggag aaaaggagaa ggaggggaag
     2461 aaggagctag aagatgagga ggatgacttt gactctttta tagcggagat gcctgctgta
     2521 gagactgtgc cttccccatt tgtggggaga gatgaggctg cctttgccag tcgccatccc
     2581 gtgaggactc aaagcacccc attcacaggc ccattcatca gcgtagtcct gtcaaagctg
     2641 gagaacatgc tggagaactc tttacatgtt aatttgctgc ttatcgggat cattactcag
     2701 ctagccagct acccccagcc actcctgcgc tcctttctgc tcaacaccaa catggtcttc
     2761 cagccaagcg tccgctctct ctatcaggtc cttgcatctg tgaaaaacaa gattgaacag
     2821 tttgcttctg tggagagaga cttcccaggg ctcctcattc aagctcagca gtacctgctc
     2881 ttccgtgtgg acatgtctga tatgacccct gcagcactaa ccaaagatcc cattcaggag
     2941 gcttccagga caggaagtgg caagaacctt ttggatggac ctccaagagt gcttcagccc
     3001 ttcctgaccc acagaaccaa ggtggctgag gcacccccca acctgcccct gccggtgagg
     3061 aaccccatgc tggctgctgc cctcttccca gagttcctga aggagctggc ggccttggcc
     3121 caggaacact ccattctgtg ctacaagatc ttgggtgact ttgaggactc ctgctgttag
     3181 ctcgacctgc agccaagcta attccgggcg aatttcttat gatttatgat ttttattatt
     3241 aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa
     3301 cg
//
